ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1993.tb01264.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Nerve sheath myxoma (NSM) is a rare cutaneous neoplasm, the histogenesis of which is controversial. Fifteen cases of NSM were studied by routine light microscopy and with a broad panel of immunohistochemical stains. NSM were classified into three groups based on cellularity, mucin content and growth pattern. 1) Thehypocellular (myxoid)type (5/15 cases) showed frequent encapsulation or sharp circumscription. Immunohistochemically this type was strongly positive for S‐100 protein and collagen type IV and variably positive for epithelial membrane antigen. 2) Thecellulartype (4/15 cases) had scant mucin and ill‐defined nodular or infiltrating growth. Immunostaining showed positive reaction for neuron specific enolase (2/4), Leu‐7 (1/4) and smooth muscle specific actin (2/4), and was negative with the oilier antibodies. 3) The “mixed type” (6/15 cases) had variable cellularity and mucin content with poor demarcation and variable immunolabeling. We conclude that: 1) there are major light microscopic and immunohistochemical differences between the classical hypocellular (myxoid) and the cellular forms of NSM (neurothekeoma); 2) while the immunohistochemical results support the presence of nerve sheath differentiation in the classical forms of NSM, and to some extent in the mixed forms, there is an absence of convincing evidence of neural differentiation in the cellular variant by either light microscopy or immunohistochemistry; 3) the variable immunophenotypes suggest that differentiation other than neural may take pla

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1993.tb01265.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

The etiology of cutaneous T‐cell lymphoma remains unknown, although an association with viral infection, in particular certain retroviruses and human herpesviruses, has been suggested. The purpose of this study was to examine skin biopsies of cutaneous T‐cell lymphoma for the presence of Epstein‐Barr virus, herpes simplex virus type 1 and type 2, and human herpesvirus‐6 by using the polymerase chain reaction. Lesional skin biopsies from 30 patients with cutaneous T‐cell lymphoma were studied. Control specimens included biopsies from 9 patients with lymphomatoid papulosis and 10 patients with pityriasis lichenoides et varioliformis acuta. DNA extracted from each specimen, as well as from a known positive control for each virus, was examined by using the polymerase chain reaction with viral‐specific primers. Each DNA specimen was also amplified with control primers for human β globin. The specificity of the amplified products was confirmed by Southern analysis. Neither Epstein‐Barr virus nor herpes simplex virus was detected in any of the patient specimens examined. Human herpesvirus‐6 was detected in one specimen of cutaneous T‐cell lymphoma and one specimen of lymphomatoid papulosis. These results do not support a role for any of these herpesviruses in the pathogenesis of cutaneo

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1993.tb01266.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Clear cell basal cell carcinoma (BCC) is an unusual variant of BCC which is characterized by a variable component of large clear cells. Twenty cases are presented which are further subdivided into pure, mixed, or metatypical categories. Although initially thought to represent sebaceous or tricholemmal differentiation, electron microscopic studies demonstrated that the clear cell changes are a degenerative phenomenon, probably involving lysosomes.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1993.tb01267.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Forty‐three patients, including 24 males and 19 females between 5 and 62 years of age, having pemphigus vulgaris (27), pemphigus foliaceus (1), bullous pemphigoid (3), chronic benign bullous dermatosis of childhood (2) and herpes zoster (10) were included in this study. Tzanck smears were prepared from the floor of the blisters in these patients by deroofing the bullae, and the slides were stored without fixation at room temperature for 1 to 10 days. Immunofluorescence staining was done with FITC‐conjugated anti‐human IgG. Twenty‐one cases having pemphigus vulgaris and 1 case having pemphigus foliaceus showed bright green fluorescence on the membrane of acantholytic cells. No epithelial cells were seen in smears from bullous pemphigoid and chronic benign bullous dermatosis of childhood, whereas epithelial cells were seen in 10 cases of herpes zoster. These stained negative with anti‐IgG. Storage of the prepared smears for 1–10 days did not seem to affect the results of immunofluorescence. Tzanck smears can be used as an easy substitute for skin/mucosal biopsy for the direct immunofluore

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1993.tb01268.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Post‐kala‐azar dermal leishmaniasis (PKDL) is caused by the protozoan parasite,Leishmania donovani, and is seen in patients with history of having been treated earlier for the visceral disease form, kala‐azar, caused by the same organism. The findings from 18 patients with PKDL are described in this study. The skin manifestations ranged from hypopigmented macules to infiltrated plaques and nodules. Histopathologic examination revealed a cellular infiltrate of lymphocytes, plasma cells, and macrophages. The macrophages were scattered amidst the infiltrate without any localization. In hypopigmented lesions, the infiltrate was confined to the perivascular region in the superficial dermis and was composed mainly of lymphocytes and few plasma cells. In the nodular lesions, the infiltrate occupied the entire thickness of the dermis. Leishman‐Donovan bodies were scarce and identified in 16 cases after a prolonged search of Weigert's iron hematoxylin‐stained sections. In 2 cases, Leishman‐Donovan bodies were not demonstrable. Electronmicroscopic study revealed parasitized macrophages which showed no structural evidence of activation despite the active cellular response around them. The fine structure of the parasites in the histiocytes was also well maintained. This unusual tropical dermatosis is a unique example of change in organotropism of a parasite associated with a change in the ho

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1993.tb01269.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

We present a study of aluminum tattoos that resulted from aluminum chloride cauterization of biopsy sites. The stippled macrophages were studied by light and electron microscopy and by means of X‐ray energy spectroscopy (XES) analysis. The light microscopic appearance may be striking, and may mimic the appearance of parasitized histiocytes, although the particles are larger and vary in size and shape. The basophilic particles are electron dense by electron microscopy. XES analysis demonstrated distinct emission peaks corresponding to aluminum, calcium, and phosphorus. Only a minor chloride emission peak was identified. The particles may represent an inorganic precipitate or an organic compound in which aluminum, calcium, and phosphate form a complex with protei

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1993.tb01270.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Infantile (juvenile) capillary hemangiomas are vascular neoplasms which can appear quite infiltrative histologically and are characterized by cords of cells with areas of marked cellularity. While vessels can be distinguished in most cases, there are many cells which do not appear to be endothelial in origin. We labeled 5 such cases with antibodies directed against factor VII‐related antigen, CD34, α actin, factor XIIIa and PC‐10. Anti‐factor VIII‐related antigen labeled all endothelial cells and did not label cells away from vessels. Anti‐CD34 recognized similar cells and also stained a subset of interstitial cells. Anti‐α muscle defined pericytes and stained few interstitial cells and none of the endothelial cells. Many of the interstitial spindled and cuboidal cells stained strongly with anti‐factor XIIIa. The majority of the mitotic activity was concentrated in the interstitial cells. These observations lend support to the concept that infantile (juvenile) capillary hemangioma is a tumor of primitive cells with capabilities for differentiating toward endothelial cells and pericytes. It is not clear whether a similar stem cell population gives rise to dermal dendrocytes, or whether these represent an immune response

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1993.tb01271.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Thirty‐one dermal appendage tumors of sweat gland differentiation including 7 spiradenomas (SPA), 8 cylindromas (CYL), 8 acrospiromas (ACS), and 8 chondroid syringomas (CS) were analyzed using antibodies to epithelial membrane antigen (EMA), cytokeratin (AE1, AE3, CAM 5.2, 34BE12), S‐100 protein, actin (ACT), and desmin (DBS) to characterize the immunocytochemical profile of benign sweat gland tumors. Cytokeratin expression was variable; AE1, 34BE12, AE3, and CAM 5.2 were present in 31, 24, 23, and 22 tumors respectively; 29 tumors contained EMA. Seventeen tumors, (6 SPA, 8 CYL, 2 ACS, 1 CS) stained with antibody to alpha smooth muscle actin, and 26 (7 SPA, 7 CYL, 4 ACS, 8 CS) expressed S‐100 protein. Although some prior studies had reported actin filaments on electron microscopy in both spiradenoma and cylindroma, these tumors have previously been considered to be negative for myoepithelial differentiation. All spiradenomas and cylindromas we studied demonstrated actin and/or S‐100 protein positivity in basal epithelial cells, consistent with myoepithelial differentiation. The organization of actin and S‐100 protein positivity displayed by the spiradenomas and cylindromas we studied suggests that the tumors are differentiated towards the secretory portion of the eccrine sw

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1993.tb01272.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

We carried out a quantitative study of the matrix and dermal papilla of the human hair follicle of the scalp, both normal and in various degrees of androgenetic alopecia. A stereological study showed the measured parameters to decrease with increase in the degree of alopecia, particularly as regards the total volume of the matrix and its papilla. The ratio of the two volumes increased by 30%, indicating a much more marked decrease in size of the papilla than in the matrix size. The number of cell nuclei in the matrix and the papilla of alopecic scalp was found to be 30% and 50% smaller, respectively, than those of normal scalp. Finally, a morphometric study revealed enlarged nuclei in the matrix and papilla, as shown by their increased areas, perimeters, and maximal diameters.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1993.tb01273.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY