ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00740.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

CD31 has recently been reported as a specific marker of endothelial differentiation among non‐hematopoietic human neoplasms. In order to address this contention in particular regard to tumors of the skin and subcutis, the authors undertook a comparative study that surveyed 145 mesenchymal lesions. The antibodies used were directed against CD31 (clone JC/70A) and CD34 (clone My 10), and these were compared with binding ofUlex europaeusI agglutinin (UEA). Proliferations that were included in the category of vascular tumors included cavernous and capillary hemangiomas (17 cases); lymphangiomas (8); epithelioid (“histiocytoid”) hemangiomas (3), papillary endovascular hemangioendothelioma (1), angiosarcoma (7), and Kaposi's sarcoma of the mixed angiomatoid and spindle‐cell type (17). CD31‐immunoreactivity was observed in 35 of 53 vascular lesions; the neoplastic cells in a single angiosarcoma and the spindle cells in each case of Kaposi's sarcoma (KS) were not labeled. In all of the latter tumors, however, staining for CD31 was identified in the endothelia of angiomatoid areas and non‐neoplastic blood vessels. These results compared favorably with those' seen with anti‐(T).9H, which decorated 36 of 53 vascular tumors ‐ including S of 17 KS cases ‐ and UEA, which bound to the neoplastic cells of 36 lesions. In contrast, all of 92 non‐endothelial tumors included in ibis study (34 nerve sheath tumors [30 benign; 4 malignant]; 39 fibrohistiocytic neoplasms 11 benign; 28 malignant]; 9 smooth muscle tumors [6 benign; 3 malignant]; 7 glomus tumors; and 3 giant cell fibroblastomas) were negative for CD31. UEA labeled 3 non‐vascular neoplasms, whereas 38 lesions of that type were CD34‐positive. The latter proliferations were benign peripheral nerve sheath tumors, examples of dermatofibrosarcoma protuberans, or glomus tumors. Based on these results, it is concluded that CD31 is a relatively sensitive and specific marker for vascular lesions and that, it is worthy of inclusion in diagnostic antibody panels which are designed to separate endothelial tumors from othe

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00741.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The papillary dermis of psoriasis and mycosis fungoides (MF) lesions is characterized by prominent collections of cells with dendritic morphology. Immunophenotypically distinct populations of cutaneous dendritic cells have been identified as CD1a+, FXIIIa‐Langerhans cells (LC) and CD1a‐, FXIIIa+ dermal dendritic cells (DDC). In this study, antibodies against the human GDI cluster of antigens (i.e. CD1a, CD1b and CD1c) and the DDC) marker (FXIIIa) were used to further characterize the subsets of dendritic cells in normal skin as compared to neonatal foreskin, psoriasis and MF by both immunoperoxidase and double immunofluorescence techniques. Normal skin and foreskin epidermis and dermis contained few CD1b+ or CD1c+ cells along with normal numbers of CD1a+ LC and FXIIIa+ DDC. Both MF and psoriasis were characterized by CD1a+ cells in the epidermis and dermis. FXIIIa+ cells were greatly expanded in the upper dermis of MF lesions and to a lesser degree in psoriasis as has been previously described by our group. MF contained significantly increased epidermal and dermal CD1b+ (15.7/5 high power fields [HPF] and 59.7/5 HPF respectively) and CD1c+ dendritic cells (33.8/5 HPF and 95.9/5 HPF respectively), while in psoriasis these cells were not statistically different from normal skin. Double immunofluorescence studies revealed that some (foreskin, while FXIIIa+ DDC never co‐expressed CD1a. Thus, in contrast to normal skin in which epidermal or dermal dendritic cells rarely express CD1b and CD1c antigens, these members of the CD1 family are upregulated on both LC and DDC in benign and malignant inflammatory states. Upregulation of CD1b and CD1c on MF epidermal and dermal dendritic cells, as compared to psoriasis, foreskin and normal skin, may be useful in the immunophenotypic recognition of MF, as well as in helping to understand its immuno

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00742.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Studies of skin involvement in Hodgkin's disease are infrequent in the literature. In particular, immunophenotypic analyses of specific cutaneous infiltrates have been performed in only a few cases. We analyzed the clinical, histological and immunohistochemical features of specific cutaneous manifestations of Hodgkin's disease comparing histologic and immunophenotypic aspects of skin lesions with those of the nodal counterpart. Seven patients with Hodgkin's disease of the lymph nodes and specific cutaneous lesions, where both nodal and skin biopsies were available for histologic and immunohistochemical analyses, were included in this study. Immunohistochemical stains were performed with a 3‐step immunoperoxidase technique on routinely‐fixed, paraffin‐embedded tissue sections. All 7 patients had nodular sclerosis Hodgkin's disease of the lymph nodes. In the skin, clinical presentations included reddish‐brown papules, plaques, nodules and ulcerated tumors. Histologic examination of cutaneous lesions showed features consistent with nodular sclerosis Hodgkin's disease in 6 cases and unclassifiable Hodgkin's disease in one. Reed‐Sternberg cells and lacunar cells were present in 4 cases (57.1%). Immunohistochemical analysis of Hodgkin's and Reed‐Sternberg cells revealed a constant positivity for CD30 (BerH2) and negativity for CD45 (LCA) in both the lymph nodes and the skin. Staining with CD15 (M1) revealed positivity in 7/7 nodal samples and 5/7 skin biopsies. Cytoplasmic expression of immunoglobulin light chains (both lambda and kappa) was observed in one cutaneous case. The accompanying infiltrate was mostly composed of T‐lymphocytes admixed with variable numbers of monocytes/macrophages and eosinophils. Our results indicate that the histology of cutaneous specific manifestations of Hodgkin's disease correlates with that of the nodal counterpart in most cases. Classical Reed‐Sternberg cells, however, can be detected only in a proportion of the cutaneous lesions. Although the immunophenotype of Hodgkin cells and Reed‐Sternberg cells in the lymph nodes and the skin is similar, CD15‐negativity of Reed‐Sternberg cells can be observed in cutaneous infiltrates

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00743.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Background– Recent studies have shown that tumor growth beyond a certain size requires angiogenesis. Microvessel density has, moreover, correlated with metastatic risk in some tumors. Invasive squamous carcinoma (SCC) can develop in the epithelium of the lip and metastasize even when relatively small. This study investigates neovascularization and its relationship to metastatic risk in this tumor.Methods– All 41 primary SCCs of the lip diagnosed at our institution from 1960 to 1991 were immunostained for factor VIII. Microvessel grade (Mv) from 1+ to 4+ and the average number of vessel profiles (TMvD) in the highest density 200 × (0.785 mm2) and 400 × (0.196 mm2) microscopic fields were determined. TMvDs were compared with those of adjacent non‐tumor tissue (NTMvD). Normalized counts (TMvDns) were calculated as TMvD/NTMvD. TMvDs and TMvDnns of metastasizing (N=10) and non‐metastasizing (N = 31) tumors were compared (studentt‐test).Results– In all SCCs TMvDs exceeded NTMvDs (50 vs.35 at 200 ×, P = 0. 0014, and 26 vs. 14 at 400 ×, P<0.0001). Metastasizing and non‐metastasizing tumors did not, however, differ in Mv, TMvD, or TMvDn.Conclusions– Angiogenesis develops, but is not quantitatively related to metastatic risk, in primary invasive squamous cell carcinoma of the lip.Tahan SR, Stein AL. Angiogenesis in invasive squamous cell carcinoma of the lip: tumor vascularity is not an indicator

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00744.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The activities of microsomal alanylaminopeptidase (APM EC 3.4.11.2) and of dipeptidyl dipeptidase IV (DPP IV EC 3.4.14.5) were histochemically studied in frozen sections of normal skin, seborrheic keratosis, basal cell carcinoma, solar keratosis, Bowen's disease and squamous cell carcinoma using amino acid‐ or peptide‐4‐methoxy‐2‐naphthylamides as specific chromogenic substrates. Compared to biochemical and immunohistochemical methods, the histochemical technique used in this study allows distinct localization of protease activity within the tumor tissue and the tumor‐associated stroma. Strong APM activity was detectable only in the stroma of basal cell carcinoma, a result which reflects the particular tumor‐stroma interaction of this semimalignant tumor. APM activity was not detectable in either healthy epidermis or the tumor parenchyma. Altered activity of DPP IV was found in the tumor cells as well as in the surrounding connective tissue: precancerous dermatoses and basal cell carcinomas had higher levels of DPP IV‐activity than normal skin or benign seborrheic keratosis. Poorly differentiated malignant squamous cell carcinomas, however, showed no histochemically detectable DPP IV‐activity at all. This result is in line with reports of decreased activity of this enzyme in ca

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00745.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

It is often difficult to predict the outcome of melanoma in patients with Clark level III‐IV disease. We sought to identify markers of cell proliferation which may be useful in predicting prognosis. Patients with Clark's level III‐IV malignant melanoma who had no local recurrences or metastases were matched with patients of comparable level and thickness who did experience recurrences or metastases. Cell proliferation markers p53, proliferating cell nuclear antigen (PCNA), and Ki‐67 were assessed by immunohistochemistry. DNA ploidy was determined by flow cytometry. There was no difference in the expression of p53, PCNA, and Ki‐67 between patients with metastases and patients without metastases. However, patients with metastases were more likely to have an aneuploid tumor cell population than were patients without metastases (p<0.03). Expression of cell proliferation markers do not appear to help predict prognosis in advanced level melanoma; however, aneuploidy may be associated with a greater probability of met

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00746.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Confocal laser scanning microscopy (CLSM) is a new optical microscopic technique, which offers significant advantages over conventional microscopy. CLSM is microscopy of optical sections. Light, which is emitted from regions other than the focal plane, is cut off by introducing a diaphragm in the beam path. The result is an optical “slice”, which shows more details because the blurring from out of focus haze disappears. It has been repeatedly used in experimental, but also in diagnostic dermatopathology. The “in vivo” confocal microscopy, applied directly to the intact skin provides details of living cells in the superficial layers comparable to that of fixed and stained tissue. While the extent of its future applications is hard to predict, its potential for applications in dermatology appears enormous, particularly for studies of fixed or living tissues, where it is desirable to obtain clear images many micrometers below the surface of the tissue under exam

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00747.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Sulfur mustard (SM), a chemical warfare agent first used early in the 20th century, has re‐emerged in the past decade as a major threat around the world. At present, there are no effective therapeutic measures for SM exposure. Because the skin as well as other interface epithelial surfaces are the first tissues effected as this agent is absorbed, reactions within the skin are an area of active research into the mechanism of action of this alkylating agent. The euthymic hairless guinea pig has been used as the animal model for the study of SM induced injuries because of morphologic similarity of its skin to human skin, with a multiple layer epidermis, and because this animal has a normal immune system. We reviewed 102 biopsy specimens from 51 animals exposed to three different dose times of saturated SM vapor. Histopathologic evidence exists for increased programmed cell death as well as cellular necrosis, subepidermal blister formation, and delayed re‐epithelialization secondary to problems with adhesion. Information obtained from this study adds to the body of information important in the investigation of the mechanisms of action of

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00748.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The authors report an example of an unusual subcuticular spindle cell tumor with histologic features which were most like those of “pleomorphic fibroma.” However, the tumor cells exhibited bifid differentiation immunohistologically, with conjoint reactivity for vimentin, S‐100 protein, Leu‐7 antigen (CD57), myelin basic protein, and desmin. A hamartomatous origin was favored for this lesion, given its overall morphologic features, circumscription, and apparently heterogeneous cellular lineages. Although speculative, it is thought that this unusual cutaneous mass may represent a subcuticular analogue of the benign Triton tumor (neuromuscular choristoma) of deep soft

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb00749.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY