摘要:

ABSTRACTDietary riboflavin deficiency is known to diminish malarial parasitemia. In this study, we determined whether imipramine and amitriptyline, drugs which inhibit riboflavin metabolism, have antimalarial efficacy. In addition, we evaluated whether these drugs, like other antimalarial agents, increase the hemolytic response to ferriprotoporphyrin IX (FP). The growth ofPlasmodium falciparum(FCR3) in the absence and presence of these drugs (10 to 75 μM) was measured by determining (3H)hypoxanthine uptake by intraerythrocytic parasites for 48 h in RPMI 1640 medium. The uptake of (3H)hypoxanthine was significantly reduced in a dose‐dependent manner by both imipramine and amitriptyline. The IC50values of imipramine and amitriptyline at 48 h were 56 and 45 μM, respectively. Both drugs enhanced hemolysis induced by FP (10 or 20 μM). No hemolysis by these drugs was detected in the absence of FP. It is concluded that the tricyclic antidepressants, imipramine and amitriptyline, possess substantial antimalarial proper

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1990.tb01116.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

ABSTRACTLocalization of the S‐antigen ofPlasmodium falciparumisolate FCQ27/PNG, from Papua New Guinea, was studied by post‐embedding immunoelectron microscopy using affinity‐purified rabbit antibodies raised against the repeat region of the antigen. Labelling was found in the parasitophorous vacuole (PV) space of early to late schizonts and in PV‐related vesicles within the erythrocyte cytoplasm of schizont‐infected cells. Other subcellular structures within the erythrocyte cytoplasm were not labelled. After breakdown of the PV membrane, label was observed around the merozoites, consistent with mixing of the PV contents and erythrocyte cytoplasm. The antigen was not found in uninfected cells, ring stages, trophozoites or associated with free merozoites. Antibodies to FCQ27/PNG S‐antigen did not react with other isolates tested, whereas rabbit antibodies to the Palo Alto/Wellcome S‐antigen repeat region reacted with isolates FCR3 and ItG2F6 but not w

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1990.tb01117.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1990.tb01118.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1990.tb01119.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY