ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05813.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

ABSTRACTMorphology and life history of 3 cephaline gregarines found in the gut of the pest of stored fruit,Oryzaephilus mercator.are described. Of these 3, 2 are new species. The 3 species are (1)Hirmocystis minuta(Ishii, 1914) (LP TL = 1:7 – 1:22, WP/WD = 1:1, 6–1:7); (2)Amsotobus indicusn. sp. (LP/TL = 1:3–1:6, WP/WD = 1:1 – 1:1.3); (3)Leidyana oryzaephilin. sp. (LP/TL = 1:2 – 1:12; WP/WD = 1

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb02673.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

ABSTRACTUluastraclurally, the cyst wall ofPneumocystis cariniiconsists of an electron‐dense outer layer, an electron‐lucent middle layer, and an innermost plasmalemma. This is similar in appearance to the cell wall of some yeasts, e.g.Saccharomyces cerevisiae, which consists of an outer dense layer of mannan, a middle lucent layer of β‐1,3‐glucan (yeast glucan) and an innermost plasmalemma. The cyst wallof P. carinii, as well as the cell wall ofS. cerevisiae, can be labeled by a variety of methods which stain polysaccharides, such as Gomori's methenamine silver (GMS) and by Aniline blue, a dye which selectively stains β‐l,3‐glucan. The treatment ofP. cariniicysts with Zymolyase, which the key enzyme is β1‐l,3‐glucan laminari‐pentaohydrolase, results in lysis of the outer 2 layers of the cyst wall and the loss of positive staining by both GMS and Aniline blue. The lysis of elements of the cyst wall ofP. cariniiis achieved under the same conditions and concentration at which Zymolyase lyses the outer 2 layers of the cell wall of viable cells ofS. cerevisiae.These observations indicate that a major component of the cyst wall ofP. ca

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05814.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

ABSTRACTThe in vitro cultivation ofPneumocystis cariniiin chick lung cell culture made it possible to observe the organism proceeding through its life cycle. It provided the foundation for extensive serocpidcmiologic studies, for in vitro drug screening, and for essential biological, metabolic, and morphologic research. In vitro culture made possible the discovery ofP. cariniiantigenemia, its biochemical nature, and its relevance to subclinical and clinical infection. Its utility in the presumptive diagnosis ofP. cariniipneumonia and in monitoring responses to drug therapy illustrate the value and clinical application of basic research.

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05815.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

ABSTRACT.A short‐term cell culture is used to propagate and purify rat‐derivedPneumocystis carinii(Pc). An aliquot of pelleted material washed out of the lungs of rats with moderate to severe Pc pneumonia is cultured for 7 to 10 days on the adherent mink lung cell line Mv I Lu, and the rest of the material is frozen down in medium with 10% glycerol. Although it has not been established that substantial multiplication of Pc occurs in culture, the Pc organisms harvested from the supernatant at the end of the culture period are relatively free of both host and feeder cells. This is in marked contrast with the lung wash inoculum in which the Pc organisms are heavily contaminated with rat cells and enmeshed in a highly sticky material. Lung wash preparations frozen down in glycerol and stored at −70° C for as long as 6 months or more can be successfully cultured upon thawing with no apparent loss of viability of the Pc org

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05816.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

ABSTRACT.Trophozoites grown in vitro were shown to undergo binary fission by transmission electron microscopy (TEM). Standard fixation with subsequent embedding in Spun‐ was employed using 3% glularaldehyde and 1% osmium tetroxide with5%sucrose added to both fixatives and 0.1 M cacodylate buffer washes. Trophozoites were grown on WI‐38 cells in vitro. Trophozoites were found in various stages of fission. The dividing trophozoite has daughter cells that arc rounder than the pleomorphic, non‐dividing trophozoites. Tubular forms external to the dividing trophozoites were decreased in number, tubular forms when present were concentrated around the forming sepia. Nuclear material was sometimes, but not always, well defined in both daughter cells. There was no concentration of nuclear material at the poles. Vacuoles without membrane were present in the dividing forms. Separate nuclear regions were sometimes found in the dividing trophozoites. These observations suggest that binary fission does occur in culture; however, the significance of binary fission to the life cycle ofPneumocystis carinii(Pc) is not yet

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05817.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05818.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05819.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

ABSTRACTPneumocystisinfection in athymic nude mice lungs showed a particularly high trophozoite to cyst ratio. A similar observation was obtained from a study of a patient with lymph node infection withPneumocystis.Eosinophilic foamy masses in these sites were observed by light microscopy . With the electron microscope, the masses were seen to be composed of large aggregates of trophozoites. Cystic forms (precyst, cyst and empty cyst) were extremely scarce in comparison with the huge numbers of trophozoites. These cystic forms were mostly undergoing degeneration. These observations indicate that the mode of proliferation in both situations was predominantly asexual, that is, proliferation by trophozoites, suggesting that certain conditions may enhance asexual reproduction or depress the formation of cysts.

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05820.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

ABSTRACTThis study describes an approach to cultivation ofPneumocystis carinii(Pc) on 2 cell lines derived from lung (A549, human and L2, rat) with emphasis on the organisms which adhered to the cells. Immunofluorescent staining was used for growth assays

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1989.tb05821.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY