摘要:

ABSTRACTAgglutinins were not detected in sera from mice given one, two, or three intranasal (i.n.) inoculations or a single intravenous (i.v.) inoculation of trophozoites ofNaegleria fowleri. However, agglutinins were produced following second and third i.v. inoculations. Serum immunoglobulin levels increased in both i.n.‐ and i.v.‐inoculated mice. IgG and IgM increased substantially more for i.v.‐inoculated mice. IgA levels increased more consistently for i.n.‐inoculat

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1982.tb02892.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACTAn unknown, ninhydrin‐positive substance detected on paper chromatograms of the endogenous metabolites of mixed rumen ciliate protozoa was isolated and purified by column chromatography with ‘Dowex’ 50‐X8 resin and identified as 2‐aminobutanoic acid (α‐amino‐n‐butyric acid) on the basis of elementary analysis, mass spectrometry, paper chromatography, infrared spectrometry, a

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1982.tb02893.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACTCellular levels of protein and two acid hydrolases, acid phosphatase (EC 3.1.3.2) and acid proteinase, were followed during cyst differentiation, arbitrarily divided into five stages, in the ciliateHistriculus muscorumKahl. Extracellular enzyme activities were also measured. Protein content decreased gradually during cyst differentiation. In mature cysts the protein content was ca. 60% that of stationary phase organisms. The activities of both acid hydrolases remained unchanged during stage 1 and then decreased gradually; acid proteinase decreased more rapidly. Both enzymes remained slightly active in the mature cysts. The acid proteinase activity of stage 1 was reduced by cycloheximide treatment at time zero, whereas the enzyme was no longer sensitive to the inhibitor when treated at 1.5 h (late stage 1) after the first wash with encysting medium. Acid phosphatase activity was insensitive to the inhibitor. Extracellular release of acid phosphatase increased linearly at least until stage 5, although the extracellular release of acid proteinase was not detected. Cycloheximide blocked the extracellular release of acid phosphatase after stage 1. These results suggest that de novo synthesis of acid proteinase occurs during stage 1 and that lysosomes may play an important role during early stages of cyst differentiation.

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1982.tb02894.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACTThe sensitivity and specificity of the indirect fluorescent antibody (IFA) test for the detection of serum antibodies were examined in mice that were infected withEimeria falciformis, E. ferrisi, E. papillata, orE. vermiformis. For the study of each species, five groups of mice were given graded inoculation doses of 10, 102, 103, 104, or 105sporulated oocysts in a primary infection. The sixth group was infected with three sequential doses of 1.5 times 103, 1.5 times 104, and 1.5 times 105sporulated oocysts per mouse at two‐ to three‐week intervals. All groups of infected mice developed serum antibodies. Sera were titrated by the IFA test with purified sporozoites. Strong fluorescence and high IFA titers were observed with homologous reactions mainly with the sera from mice infected with the higher inoculation dose levels in primary infections and from those given three sequential inoculation doses. Immunological cross reaction among the four species ofEimeriaoccurred at dilutions of 1:10 to 1:160. Very weak or no fluorescence of free sporozoites was observed with sera from noninfected mice, and there was no fluorescence of sporozoites contained in intact sporocy

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1982.tb02895.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACTA new method is described for mass cultivation ofEuplotes aediculatus, a hypotrich ciliate containingomikron‐symbionts. The ciliate cultures, continuously aerated in Erlenmeyer flasks (5000 ml) with 4500 ml medium, yield densities of 2300 cells/ml which are four to five times higher than cell densities of cultures grown in unaerated Fernbach flasks. Harvesting such cultures involves the application of 25‐μm mesh sieves. Cells so concentrated can be purified by using columns or special chambers in whichEuplotesmigrates towards the cathodes in an electric field (field strength 7 V

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1982.tb02896.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACTStocks ofEntamoeba histolyticagrown in a monoxenic culture system from the feces of nonhuman primates are compared with the eleven zymodemes ofE. histolyticaso far demonstrated from man. In a similar fashion,Entamoeba chattonihas also been grown and identified. BothE. histolyticaandE. chattonihave been demonstrated in keepers of the primate collections. Comparisons have been made using the electrophoretic patterns of three enzymes: glucosephosphate isomerase [(GPI) E.C.5.3.1.9], phosphoglucomutase [(PGM) E.C.2.7.5.1], and L‐malate—NADP+oxidoreductase (oxaloacetate‐decarboxylating) [(ME) E.C. 1.1.1.40]. Enzyme patterns ofE. histolyticafrom the apes were found to be identical with three of those already demonstrated from man. The enzyme pattern ofE. chattoniwas distinctly different from that of any of theE. histolyticazymodemes. Other protozoa found in the single fecal sample examined from each subject are also l

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1982.tb02897.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Book reviews in this article:Dillon, Lawrence S.1981.Ultrastructure, Macromolecules and EvolutionSchwemmler, Werner&Schenk, Hainfried E. A., eds. 1980.Endocytobiology: Endosymbiosis and Cell Biology, a Synthesis of Recent ResearchKrylov, M. V.&Starobogatov, Y. I., eds. 1980.Principles of the Construction of the Macrosystem of the Unicellular AnimalsPalmer, W. J., ed. 1981.Rapid&Automated Methods in Microbiology&Immunology: A Bibliography, 1976–1980de Harven, E.&Nemanic, M. K., organizers. 1981.Cell Surface LabelingInoki, Shozo, ed. 1981.Atlas of ProtozoaCzapik, Anna1980.Pdostawy Protozoologii. [Introduction to Protozoology.]Levine, Norman D.&Ivens, Virginia1981.The Coccidian Parasites(Protozoa, Apicomplexa)of CarnivoresPage, Frederick C.1981.The Culture and Use of Free‐Living Protozoa in Teac

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1982.tb02898.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY