摘要:

SYNOPSISA method is described for the axenic mass cultivation ofParamecium tetraureliastrains 51s and 299s. the ciliate is grown in an enriched axenic medium developed by Soldo, Godoy&van Wagtendonk. Under continuous shaking on a rotary shaker, cultures were grown in one‐liter Erlenmeyer flasks with 330 ml medium yield cell densities of 32,000 cell/ml and 20,000 cells/ml for strains 299s and 51s respectively. Doubling time is considerably shorter under these conditions than in the conventional static cultures. A 20‐liter airlift bioreactor is described in detail which can be used successfully to otain up to 100 g wet weight ofParameciumin a single run; in this reactor the cell density reaches 38,000 cells/ml for strain 299s. and 23,000 cells/ml for 51s. This technic should facilitate the study of minor protein components of the cili

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb04240.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SYNOPSISHydroxyurea (HU) concentrations above 50 μg/ml reversibly inhibited cell division ofTrypanosoma brucei bruceistock STIB 366 procyclic culture forms, but not growth of individual cells. the volume of the nucleus and of the cytoplasm increased in the presence of the drug as did the percentage of cells with 2 kinetoplasts. Electron microscopy revelaed that the nuclear membrane was extended forming protrusions which surrounded areas of the cytoplasm. Replication of the kinetoplast DNA did not seem to be affected by HU. the uptake of [3H]thymidine increased in the presence of 25 μ/ml HU 3‐fold compared to control cells. the nuclear volume and the dry weight of the culture forms increased proportionally to the amount of label incorpora

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb04241.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SYNOPSISThe cadmium ion (Cd2+) was accumulated byAmoeba proteusin all cellular fractions, the highest level being associated with the cytosol fraction. On gel separation of the cytosol fraction, Cd‐binding protein appeared in 2 peaks: one>45,000 MW (peak I) and the other 12,000 MW (peak II). Added cysteine increased the total Cd2+taken up by the cells and resulted in disproportionate increase of Cd incorporated into the Cd‐binding protein of peak II. the Cd‐binding protein of peak II is analogous to the low‐MW, Cdbinding proteins inAnacystis nidulans, Mytilus edulis, and to the metalloprotein of some vert

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb04242.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SYNOPSISCatalase activity ofParamecium tetraureliadecreased during autogamy and recovered to normal 5 days later. Autogamy also caused changes in the ciliate's sensitivity to natural ionizing radiations—the decrease in cell growth rate previously described in shielded cultures did not occur when autogamous cells were used. Maximum effect of shielding was observed in 11‐day‐old postautogamous cells. the role of the catalase in the mechanism of natural irradiation effect is disc

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb04243.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb04244.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb04245.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY