摘要:

SYNOPSIS.Traditionally, observations on the nature of protozoa have been published in periodicals or books, or remain buried in research notebooks. The retrieval and processing of information on a particular species or strain are dependent solely upon individual investigators. Although various modern methods have been applied to the study of protozoa, no attempt has been made to develop a system with which information on protozoan strains can be stored, retrieved easily, and processed for various analyses by computer technology. Based upon an existing system for encoding data on bacterial strains, a complementary system applicable to protozoan strains was developed and is described herein.

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb05374.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SYNOPSIS.Dipodomys merriamiMearns andDipodomys ordiWoodhouse were surveyed for coccidia in El Paso County, Texas. Infections withEimeria chobotari. Eimeria dipodomysisandEimeria balphaewere 24.8%. 4.4%, and 11%, respectively, forD. ordi. Dipodomys merriamihad an infection level of 23.8% withE. chobotari. Four animals concurrently harboredE. chobotariandE. balphaeorE. dipodomysisor a new species Eimeria chihuahuaensis. Male and female host infection levels were not significantly different. The new species is described and photographs of 3 previously describedEimeriafromDipodomysare presented.

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb05376.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SYNOPSIS.A survey of 41 herbivorous mole‐rats,Spalax ehrenbergiNehring, in Urfa, Adiyaman, and Maras provinces of Turkey revealed 7 new species ofEimeriain addition to previously described Eimeriidae. The shape, average dimensions (in μm) of their oocysts, and the numbers of hosts from which the new species were isolated were as follows: Eimeria urfensis sp. n., ellipsoidal (33 × 21), from 8 hosts; Eimeria adiyamanensis sp. n., ovoid to ellipsoidal (33 × 18), from 6 hosts; Eimeria haranica sp. n., elongate ovoid (37 × 20), from 22 rats; Eimeria marasensis sp. n., ellipsoidal (36 × 18), from 2 rats; Eimeria oytuni sp. n., pear‐shaped (24 × 17), from 2 hosts; Eimeria celebii sp. n., ellipsoidal (16 × 9), from 1 rat; and Eimeria torosicum sp. n., spherical to subspherical (11 × 10), fro

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb05377.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SYNOPSIS.Pfeifferinella gugleri sp. n. was found in the liver of each of 2 land snails,Triodopsis albolabris(Say), in Iowa. The stages found, their average length × width dimensions (in μm), and their principal features were as follows: oocysts (21 × 14.5) ovoid, with micropyle and oocyst residuum; meronts (20 × 15) with 24–32 merozoites: microgametocytes (15 × 11.5) with many small microgametes; macrogametes (20 × 10.5) with 1–5 nucleoli and 1–2 wall‐forming bodies, but without a “vaginal [fertilization] tube.” This tube, originally described by Léger&Hollande, 1912, fromPfeifferinella impudica, has since been considered a principal taxonomic feature of the family Pfeifferinellidae. In the absence of this structure, the taxonomic status of this monogeneric family is reexamined with the recommendation that the family be retained as distinct, but redefined to exclude the “vaginal tube” as a valid characteristic. The discovery ofP. gugleribrings to 3 the number of pfeifferinellids reported, the other 2 beingP. impudicaLéger&Hollande, 1912, andP. ellipsoidesWasielewski, 19

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb05378.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SYNOPSIS.Promastigotes ofLeptomonassp., a flagellate parasite of the silkworm,Bombyx mori, multiplied by binary fission with the following sequence of events: duplication of the flagellum; division of the kinetoplast and the nucleus; spatial separation of the kinetoplast: and cytokinesis resulting in the formation of 2 daughter promastigotes. In the early stages of encystment, promastigotes aggregated in a rosette and assumed a stumpy form. The nucleus and kinetoplast of the stumpy promastigotes were double, suggesting a possibility of fusion of the organism in the rosette. When most of the promastigotes in the cluster became stumpy, each individual was isolated from the cluster and acquired a thick coat with an acidophilic substance, thus forming a cyst.

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb05379.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SYNOPSIS.Mature macrogamonts were present in the small intestine of rats 5.5 to 7.5 days postinoculation withEimeria nieschulzioocysts; oocysts were present at 6 to 7.5 days. Types I and II wall‐forming bodies in macrogamonts began to undergo ultrastructural changes within zygotes to form the outer and inner layers of the oocyst wall. Before and during oocyst wall formation a total of 5 membranes (M1–5) were formed at or near the surface of the zygote. The outer and inner oocyst wall layers formed between M2 and M3, and M4 and M5, respectively. The mature oocyst was loosely surrounded by M1 and M2, had an electron‐dense outer layer, 100–275 nm thick, and an electron‐lucent inner layer, 160–180 nm thick. It also contained an electron‐lucent line consisting of M3 and M4 interposed between the outer and inner layers of the oocyst wall. The micropyle, measuring 935 × 47 nm, was located in the outer layer of the oocyst wall and consisted of 10–14 alternating layers of electron‐dense and lucent material. The sporont of mature oocysts was covered by M5, immediately beneath which were M6 and M7. The sporont contained a nucleus and nucleolus, lipid and amylopectin bodies, mitochondria, ribosomes, as well as smooth and rough endoplasmic reticulum. Canaliculi, Golgi complexes, and types I and II wall‐formin

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb05380.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SYNOPSIS.The development ofSarcocystis cruziHasselmann (syn.S. fusiformisRailliet) meronts was studied in seven 7‐ to 10‐day‐old calves killed 4, 7, 11, 15, 22, 25 and 28 days postinoculation (DPI) with 5 × 107sporocysts from feces of coyotes. No meronts were found 4 and 7 DPI. Young and intermediate meronts with 1–16 nuclei were found in endothelial cells of arteries in mesenteric lymph nodes, but not in kidneys 11 DPI.Mature meronts were noted in endothelial cells of arteries, arterioles, or capillaries of many organs of calves killed 15 to 25 DPI. No first‐generation meronts were found 28 DPI. By electron microscopy, all stages of the first‐generation merogony were found free within the host cell cytoplasm and not within a parasitophorous vacuole. The appearance of intranuclear spindles preceded the formation of merozoites by endopolygeny. Mature meronts measured 41.0 × 17.5 (34–50 × 15–24) μm, contained ∼ 100–350 merozoites, and had 2 to 4 relatively small residual bodies, 2.8 μm in diameter. Merozoites measured 6.3 × 1.5 (5.5–7 × 1 μm) and contained most of the organelles characteristically found in coccidian merozoites. Micropores were observed in merozoites, but not in young and intermediate meronts. Merozoites were seen free in the lumen of blood vessels, in intracellular areas, and free w

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb05381.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SYNOPSIS.Experimentally induced precystic stages and mature cysts from 3 clones ofTetrahymena rostratawere examined by light and electron microscopy. It was demonstrated by cytochemical staining and fine‐structural observations that precystic stages release mucocyst material that provides for the production of a cyst wall. Early and late cysts also contain numerous autophagous vacuoles. In late cysts there is a replacement of depleted mucocyst organelles. The developmental evidence obtained from sampling of sequential developmental stages suggests an ∼24‐h timetable of cytoplasmic events associated with encystment in this org

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb05382.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SYNOPSIS.Differences in the composition and distribution of cell membrane carbohydrates were demonstrated in the 3 life cycle forms of 3Trypanosoma cruzistrains by using lectins with different specificities. The results suggest that lectin binding may be useful in characterization of the parasite strains.

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb05383.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SYNOPSIS.Filamentous cyanobacteria are ingested through the cytopharynx of the ciliatePseudomicrothorax dubius.The cytopharynx is a complex of microtubules and microfilaments located in a highly vesiculated cytoplasm, the phagoplasm. Two types of membrane‐bounded phagoplasmic vesicles can be distinguished by their differences in size, fine structure, and acid phosphatase (AcPase) content. One type has a homogeneous, electron‐dense interior which is AcPase‐positive. These vesicles are present in fed cells and in unfed cells devoid of food vacuoles, and thus appear to be primary lysosomes. During phagocytosis, exocytosis within the cytopharynx of the primary lysosomes results in the elaboration of a food vacuole. The vacuole grows by incorporation of lysosomal membrane; lysosomal hydrolases are liberated into the vacuole. Within less than 1 second of AcPase's entry into the food vacuole, it is detectable within the cyanobacterial cytoplasm, and within 5 seconds, destruction of the cyanobacterial filament is observed. It is hypothesized that the rapidity of hydrolase penetration of the cyanobacterial cell wall is the result of the action of molecules analogous to the “killing agents” of neutrophil leukocytes, which rapidly render bacterial envelopes permeable. AcPase, and presumably other hydrolases, are present in the cyanobacterial filament when filament destruction occurs; they thus appear implicated in this process. Hydrolases may activate an autodestruction mechanism in the cyanobacterium. Firm adherence of the food vacuole membrane to the cyanobacterial filament is demonstrated, and its role in phagocytosis is

ISSN:0022-3921    

DOI:10.1111/j.1550-7408.1980.tb05384.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY