摘要:

This review highlights various aspects of the new functional classification of pituitary adenomas which is based on detailed immunohistochemical and ultrastructural analysis and correlation with clinical and biochemical findings. In addition, current investigation of the non‐hormonal aspects of these tumours is discussed, includins the application of flow cytometry in tumour ploidy studie

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb01841.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Eight examples of histiocytic necrotizing lymphadenitis without granulocytic infiltration (Kikuchi's lymphadenitis) are described. They occurred in young or middle‐aged women who usually complained of latero‐cervical lymphadenopathy. Serology revealed significant titres for Epstein‐Barr virus andYersinia enterocoliticaserogroup 9 in one of eight and one of six tested. All patients fully recovered within 2 months. On histological examination of the lymph nodes large foci of infiltration were observed in the cortex and/or paracortex: they consisted of variable numbers of small lymphocytes, immunoblasts, macrophages and so‐called plasmacytoid T‐cells; granulocytes were absent. Necrotic changes varied from single pyknotic cells to extensive areas of necrosis. Immunohistochemistry showed that within the lesion the number of macrophages was inversely proportional to the number of peripheral T‐lymphocytes and ‘plasmacytoid T‐cells'. The latter displayed a phenotype (CD4+, CD10+, CD45+) which, in the absence of macrophage‐associated antigens, seemed in keeping with their supposed lymphoid nature. In seven cases peripheral T‐lymphocytes predominantly expressed the cytotoxic/suppressor phenotype, while in one remaining case a mild predominance of the helper/inducer subset was observed. In the areas with less extensive tissue necrosis, numerous T‐immunoblasts expressed both markers of activation and the proliferation‐associated nuclear antigen Ki‐67. The results of the present study expand the spectrum of our knowledge and allow speculation as to th

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb01842.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The mononuclear phagocytic system is composed of cells which display a marked immunohistological heterogeneity. In the present study we have investigated the immunohistochemical and enzymatic features of macrophages and accessory cells present in human lymph nodes and spleen and, as control tissues, in thymus, liver, skin and heart. Our investigation has demonstrated that macrophages present in germinal centres display an immunophenotype different from that of macrophages populating T‐dependent areas. Furthermore, cells lining lymph node sinuses and splenic sinusoids express endothelial and macrophage markers, and are able to modulate their immunephenotype according to different reactive conditions. These data suggest, on immunohistochemical grounds, that macrophages populating B‐and T‐dependent areas as well as sinuses of human peripheral lymphoid tissues, may modulate their immunophenotype according to environmental and antigenic influ

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb01843.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Using a large range of monoclonal antibodies to specific cluster differentiation antigens the phenotypes of a series of high‐grade non‐Hodgkin's lymphomas of B‐and T‐cell type were investigated. Cell ploidy and proliferative fraction were assessed by fluorescent staining of DNA and flow cytometry and data on the incidence of complete clinical remission were obtained. With the exception of some lymphoblastic lymphomas, high‐grade B‐cell lymphomas normally expressed the pan B‐cell antigens CD 19 and CD22 but only immunoblastic lymphomas consistently expressed the pan B marker CD20. Variable, generally weak expression of CD21 was observed whilst CD23 expression was most prevalent in rapidly proliferative cases and in Burkitt's and centroblastic lymphomas. A rapidly proliferative, multilobated B‐cell lymphoma displayed phenotypic properties intermediate between centroblastic and immunoblastic lymphomas. The T‐cell lymphomas generally showed low proliferative activity and expression of CD4 prevailed over CD8. Most cases also showed CD2 and CD5 positivity with some also showing CD3 and CD7 expression. Patients with rapidly proliferative diploid or DNA aneuploid tumours obtained complete remission more readity than patients with lowly proliferative diploid tumours. An excess of early deaths occurred a

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb01844.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The diagnosis of Hirschsprung's disease relies upon histology and acetylcholinesterase histochemistry of the enteric neural plexi. A distinctive neurofilament protein staining pattern has been claimed in Hirschsprung's disease. We studied 10 colons affected by Hirschsprung's disease, together with appropriate controls using antibodies to neurofilament protein (NFP; monoclonal), neurone‐specific enolase (NSE), glial fibrillary acidic protein (GFAP) and S‐100 protein (all polyclonal), and conventional histology and histochemistry, seeking an immunohistochemical diagnostic method. We found staining for NFP, NSE and S‐100 protein of many of the nerve fibres and satellite cells in the enteric plexi, but without significant differences between affected and unaffected colons. Staining for GFAP was weakly positive in a minority of cases and controls and the majority of neurones in control sections stained for NSE. In contrast to acetylcholinesterase little staining was localized in the lamina propria. Staining for NSE and S‐100 is useful in identifying immature ganglion cells in paediatric large in

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb01845.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

We report a novel abnormality affecting simple mucin‐secreting cells of the glands of pyloric gastric mucosa and of Brunner's glands. Subnuclear vacuolated mucous cells show a distinctive appearance on haematoxylin and eosin staining. They are columnar cells of similar size to simple mucin‐secreting cells, but have a central nucleus beneath which the cytoplasm has a uniform glassy eosinophilic appearance or contains a clear area. Subnuclear vacuolated mucous cells are found focally lining the lower third of pyloric gastric glands or in Brunner's glands, and may be mistaken for a form of metaplasia. Histochemically, the apical portion of the cell stains for neutral mucin, but the basal portion stains only weakly and variably for protein. Electron microscopy reveals that the basal portion consists of a large, single, membranebound vacuole, variably indented by the nucleus, and probably derived from either endoplasmic reticulum or the Golgi apparatus. The vacuole contains granular material which varies in electron density from cell to cell. The apical portion of the cell contains organelles similar to simple mucin‐secreting cells. These appearances are highly suggestive of an abnormal accumulation of non‐glycoconjugated mucus core protein. Although the cause of this is unknown, it was associated with the histological changes of chronic gastritis in 10 of our 1

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb01846.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

A rapidly fatal bladder tumour which had the features of a rhabdoid tumour was studied by sequential biopsies and at autopsy. This is the first rhabdoid tumour recorded at this site and the first in which there was co‐existent transitional cell carcinoma. The possibility that rhabdoid tumour is histogenetically heterogeneous is discusse

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb01847.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Systemic necrotizing vasculitis involving cerebral blood vessels is described in a 30‐year‐old man with rheumatic heart disease and subacute bacterial endocarditis. Fever, anaemia, splenomegaly and positive blood cultures for Gram‐negative bacteria were found on admission. The fever resolved with antibiotic therapy on the third hospital day but he then developed hemiplegia and multifocal seizures. The seizures progressed to uncontrollable status epilepticus accompanied by congestive heart failure and the patient died 20 d after admission. At autopsy, exudative and necrotizing vasculitis involving medium‐ to small‐sized arteries was seen in the brain, the heart and the skeletal muscles. Rheumatic myocarditis and endocarditis and old rheumatic mitral valve deformities were also present. In addition, verrucous endocarditis in the mitral valve and Löhlein's focal glomerulonephritis were noted. We discuss the possible mechanism of the systemic necrotizing vasculitis in relation to rheum

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb01848.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Book reviewed in this article.Lung CarcinomasEdited by Elizabeth M.McDowell. Churchill Livingstone, Edinburgh, 1987, 410 pages, $40.00Adipose Tissue Tumors. The American Society of Clinical Pathologists Classic Teaching Collections Soft Tissue Series No. 6F.M. Enzinger, E.H. Soule&R. Lattes. American Society of Clinical Pathologists Press, Chicago (distributed by Raven Press, New York outside the US and Canada), 1986, 17 pages, $75.00Striated and Smooth Muscle Tumors. The American Society of Clinical Pathologists Classic Teaching Collections Soft Tissue Series No. 4E.H. Soule, R. Lattes&F.M. Enzinger. American Society of Clinical Pathologists Press, Chicago (distributed by Raven Press, New York outside the US and Canada), 1987, 33 pages, $75.00Pathology of Neoplasia in Children and AdolescentsM. Finegold. W.B. Saunders, Philadelphia, 1987, 481 pages, $60.00Cytochemistry of the Gas‐Exchange Area in Vertebrate LungsC. Meban. Gustav Fischer Verlag, Stuttgart, 1987, 57 pages, DM 56.00Pigment Cell, Vol. 8, Pathobiology of Malignant MelanomaEdited by D. Elder. S. Karger, Basel, 1987, 225 pages, $79.10Concise Renal PathologyF.E. Dische. Castle House Publications, Tunbridge Wells, 1987, $48.

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb01849.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb01850.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY