摘要:

Monoclonal antibody Ki‐67 is a reliable and easy means of accurately assessing the growth fraction of human neoplasms. Although the number of long‐term follow‐up studies is limited, it does appear to provide valuable progrostic information particularly in lymphoproliferative disease. Since the estimation of growth fraction is only one factor influencing tumour behaviour it would be naive to believe that measurement of this parameter alone, no matter how accurately, would provide the clinician with definitive prognostic information for all tumours. The antibody is also of use in research, providing a means of measuring proliferative activity in a variety of conditions besides malignancy, and may prove of value in monitoring tumour response to established and trial ther

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1990.tb00788.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

This study was performed to answer the question: which parts of breast cancers are active in terms of proliferation as measured by the Ki‐67 antibody and in terms of cell division as measured by the mitotic index. Forty‐six breast samples were studied, including 34 breast cancers and 12 benign conditions. The intraductal component of infiltrating breast cancers showed a significantly lower proliferation index than the infiltrating component. The cells at the periphery of infiltrating tumour strands showed a higher proliferation activity than the cells in the core. These findings suggest that infiltration advances through preferential active growth of the cells at the invasion fr

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1990.tb00789.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A histological study was made of the carotid bodies obtained at necropsy in 40 fetuses, neonates, infants and young children, ranging in age from 23 weeks of gestation to 11 years of age. Prominence of the dark variant of chief cells was found in diseases such as bronchiolitis which are associated with sustained hypoxaemia. Such cells have copious cytoplasm known to be rich in peptides such as enkephalins. The same histological features were found in two cases clinically diagnosed as sudden infant death syndrome but in which there was associated bronchiolitis and tracheobronchitis respectively, thought to have given rise to hypoxaemia. In a case of sudden infant death syndrome without infection there was overgrowth of sustentacular cells with predominant progenitor cells in the absence of dark cells. We associate this appearance with inability of the carotid bodies to respond adequately to changes in arterial oxygen tension.

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1990.tb00790.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

The infantile cholangiopathies are a group of conditions associated with neonatal jaundice, which include extrahepatic biliary atresia, paucity of intra‐hepatic bile ducts and disorders associated with persistence of fetal biliary structures, the so‐called ductal plate malformations. Although previously regarded as distinct entities, it has recently been suggested that they may represent parts of a disease spectrum in which the principal process is one of bile duct destruction, the morphological manifestations in individual cases being influenced by the stage of intra‐uterine development at which such injury occurs and by the site within the biliary system at which there is maximum damage. To further examine this concept, we have studied liver biopsy specimens from 37 neonates with extrahepatic biliary atresia, with particular reference to abnormalities of the intrahepatic bile ducts. Paucity of intrahepatic ducts, defined as a bile duct: portal tract ratio of less than 0.9, was identified in six cases (16.2%). In eight cases (21.6%) we found concentric tubular ductal structures similar to those observed in ductal plate malformations. In one case, both abnormalities could be demonstrated. Our findings support the concept that there is overlap between the various types of infantile cholangio

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1990.tb00791.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

The histological, mucin histochemical and immunohistochemical features were evaluated of 74 solitary and 73 synchronous colorectal adenomas which were endoscopically removed from 124 patients. Of the patients, 60% had a single adenoma, whereas 40% had at least two adenomas in their colorectum. Comparing the incidence of synchronous adenomas in both sexes revealed a statistically significant higher incidence (P<0.005) in males. The localization of the solitary and syn hronous adenomas in the large bowel was similar. Moreover, parameters of malignant change within the adenomas (size, predominant type of mucosal growth and degree of dysplasia—with the exception of severe dysplasia) as well as signs of dedifferentiation (relative proportions of goblet and columnar cells) were also similar. Mucin staining intensities (periodic acid‐Schiff, high iron diamine and alcian blue) and the immunoreactivity patterns of secretory component and carcinoembryonic antigen, both cytoplasmic and on the surface of the epithelial cells, were also identical in both groups of adenomas. Thus, neither the routine histological nor the mucin‐and immunohistochemical features differed between the groups, except for severe dysplasia. It is concluded that there is no inherent difference in malignant potential between solitary and synchronous adenomas, with the possible exception of the degree of dysp

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1990.tb00792.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A etrospective study was performed on gastric carcinomas to establish the prevalence ofHelicobacter pyloriinfection in gastric epithelium adjacent to the tumour. A total of 105 carcinomas were studied. The overall prevalence ofHelicobacter pyloriinfection was 59%. The prevalence in different age cohorts from patients with gastric carcinoma was compared with that in patients suffering from non‐ulcer dyspepsia and, based on serological testing, with that in healthy blood donors. The presence ofHelicobacter pyloriin cancer patients aged 41–50 and 51–60 was significantly higher than in blood donors. No difference was seen in comparison with non‐ulcer dyspepsia patients. The presence ofHelicobacter pylorishowed an inverse correlation with the extent of intestinal metaplasia. The intestinal type of carcinoma was associated with a higher bacterial load than the diffuse type. These data suggest that the presence ofHelicobacter pyloriin gastric mucosa could play a role in the pathogenesis of gastric carcinoma, especially in the younger ag

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1990.tb00793.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

During repair of 12 atherosclerotic abdominal aortic aneurysms, fresh samples of aneurysm wall were obtained. Histology confirmed the presence of advanced atherosclerosis associated with medial thinning and a variable aortic adventitial chronic inflammatory cell infiltrate. Monoclonal antibodies were used to identify the inflammatory cells throughout the aortic wall. The majority of lymphocytes in the aortic adventitia were B‐cells. B‐cells were not present in atheromatous plaques. T‐cells, predominantly T‐helper cells, were found in atheromatous plaques and in aortic adventitia. The majority of lymphocytes and macrophages in aortic adventitia and most vascular endothelial cells were HLA‐DR positive. Ki‐67 staining was found in B‐cells and T‐helper cells, indicating that these cells were proliferating. Occasional lymphocytes were BerH2 positive, indicating that some lymphocytes were activated. These findings suggest that chronic periaortitis is an active, immunoiogically mediated, local complication of advanced human

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1990.tb00794.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Frozen sections from 35 T‐cell lymphomas were stained with the Diversi‐T αβ T‐Cell Receptor panel which includes seven antibodies to T‐cell receptor variable region gene products. In five cases a monoclonal population of T‐cells could be demonstrated (one case Vβ5 +, three cases Vβ8 + and one case Vβ6 +) and in a further case a biclonal population (Vβ5 + and Vβ8+). We conclude that this antibody panel is of limited usefulness for the demonstration of clonality in

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1990.tb00795.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

This study was undertaken to assess whether mild cervical intraepithelial neoplasia (CIN 1) lesions are histologically overdiagnosed and, if so, what the possible reasons are for this. The magnitude of the discrepancy between the histological diagnosis of CIN and corresponding smear results was also investigated. Between January and April 1988, 282 patients were found to have a histological diagnosis of CIN 1. The cervical smear result was negative in 42% of cases, borderline in 14% and in the remaining 44% various grades of CIN were predicted. Review of both cytology smears and histology sections was undertaken to identify reasons for this discrepancy. The results suggest that: (1) there is a tendency among histopathologists to overdiagnose cases of CIN 1, as occurred in 10% of cases in this study; (2) even if strict morphological criteria are followed, there will always be cases where histopathologists are uncertain whether a lesion is CIN 1 or some reactive process—for these cases, 22% in our study, the term borderline is recommended; and (3) cervical smears may give false negative results, as in 35%, 18% and 3% of our cases of CIN 1, CIN 2 and CIN 3 respectivel

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1990.tb00796.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

We report the results of a histological evaluation of 335 consecutive autopsy specimens of apparently normal urinary bladder for the presence of a complete, partial or minimal muscularis mucosae. There were 164 females (49%) and 171 males (51%), ranging in age from 20 weeks of gestation to 102 years. A muscularis mucosae was present in 117 bladders (35%). The female to male ratio was 2:1, with 45% of female and 25% of male bladders containing a muscularis mucosae. The muscularis mucosae was complete in one case (1%), partial in 23 cases (6%) and minimal in 93 cases (28%). The occurrence of a partial or complete muscularis mucosae was ten times more likely in women than men. The presence of a muscularis mucosae in both women and men was not associated with any known disease process, nor was it related to patient age.

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1990.tb00797.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY