摘要:

The epithelial, vascular and lymphoid components present in two cases of adenolymphoma (Warthin's tumour) were characterized by immunohistochemistry on frozen sections. Epithelial cells were positive for cytokeratin and keratin and were in close contact with numerous factor VIII related antigen‐positive capillary vessels, running parallel to the epithelial basement membrane. The capillary vessels were in communication with high endothelial post‐capillary venules which were often lined by HLA‐DR‐positive endothelial cells. The lymphoid tissue was organized in B‐cell and T‐cell areas. The B‐cell areas consisted of B4+/To15+ B‐lymphocytes admixed with DRC‐1–positive follicular dendritic reticulum cells. The T‐cell areas were mainly populated by T11+/T3+/T4+ lymphocytes admixed with some S‐100+/HLA‐DR+ interdigitating reticulum cells. Plasma cells and T8+ lymphocytes were more numerous in the proximity of the epithelium. Leu‐M3+/PAM‐1+ macrophages were scattered singly in the T‐cell areas or were arranged in sinus‐like structures around the epithelial cysts. The epithelial basement membrane, the vascular membrane and the fibrillar reticular stroma of the lymphoid tissue were immunoreactive for laminin and type IV collagen. The possibility is discussed that the accumulation of lymphoid tissue in Warthin's tumour is modulated by the epithelial cells, perhaps through their ability to organize a pe

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb02666.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Forty‐three cases of large cell anaplastic thyroid carcinoma were examined with various antisera. Four histological patterns were identified: spindle cell, giant cell, trabecular and squamous. In 38 cases the epithelial origin was demonstrated with various epithelial markers: 11 cases stained positively for thyroglobulin, 19 for T3and/or T4, 35 for fat globule membrane antigens, 28 for keratin, 29 for lactoferrin, and one for calcitonin. Five cases were negative for all epithelial markers but could not be characterized further since, except for vimentin, they did not have mesenchymal markers. The immunohistochemical proof of the epithelial nature of anaplastic thyroid tumours is given by staining with anti‐keratin and anti‐fat globule membrane antigen. In addition the detection of lactoferrin seems useful, but not that of thyroglobulin which was present in only 4% of the spindle cell tumours and in 32–56% of the other histological types. Thirty carcinomas were positive for vimentin; the co‐expression of vimentin and epithelial markers seems frequent in thyroid anaplastic c

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb02667.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Fine needle aspiration (FNA) cytology is a valuable aid to diagnosis and tumour staging in patients with non‐Hodgkin's lymphoma. These tumours are often multicentric and involve sites such as the liver or the spleen which are not easily accessible to surgical biopsy. Particularly with splenic involvement, there is a diagnostic problem of morphologically distinguishing the lymphoma cells in an admixture of normal lymphocytes. Since most lymphomas in adults are of B‐cell origin, we studied the diagnostic value of adding a surface immunoglobulin (sIg) light chain analysis to the cytological evaluation of FNAs. B‐clonal excess was determined by flow cytometric analysis of the sIg light chain distribution and a monoclonal finding was considered diagnostic of lymphoma. In primary diagnostic procedures the light chain analysis established a diagnosis of lymphoma in 5/14 (36%) aspirates from patients with poorly differentiated tumours. Fine needle aspirates performed as part of staging procedures were morphologically normal or inconclusive in 19 cases; in seven of these (37%) lymphoma involvement was diagnosed by the light chain analysis. Diagnostic precision was enhanced by combining morphological and immunological evaluation of fine needle aspirates in patients with established or suspected non‐Hodgkin's l

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb02668.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Monoclonal antibodies were produced against a suspension of formaldehyde fixed human epidermal cells. The supernatant fluid of one clone (BG3C8) yielded a bright immunofluorescent staining of basal cells both in cryostat sections of human split skin and in preparations of purified basal cells. As determined by one‐ and two‐dimensional gel immunoblotting of epidermal basal cell proteins the antibody recognized a minor basic poiypeptide of 55000 apparent molecular weight that was not present in extracts of cultured cell lines of epithelial, fibroblast and lymphoid origin. The distribution of the 55000 molecular weight protein in normal human tissue was determined by immunohistological staining of cryostat tissue sections that included: central nervous, endocrine, female and male reproductive, alimentary, lymphatic‐haemopoietic, respiratory and urinary systems, skin and its appendages, mesenchymal tissue (bone, cartilage, muscle, connective tissue, blood vessels, nerves and synovia) as well as placenta and umbilical cord. The results showed a restricted distribution of this antigen which was found only in basal cells of most stratified or pseudostratified epithelia and in myoepithelial cells. This antibody may be useful in the study of normal and pathological differentiation in various epithelial diso

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb02669.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Zinc alpha2glycoprotein is one of the proteins present in breast cyst fluids, being found at levels 30–50 times its plasma concentration. Using an immunoperoxidase technique the distribution of this glycoprotein has been studied in a range of non‐mammary tissues and carcinomas, as well as in normal, benign and malignant breast specimens. The breast cyst fluid protein was detected in all apocrine cells of skin and in the apocrine metaplastic epithelium lining of breast cysts. A progression was apparent from normal to hyperplastic breast in the number of cells reacting, particularly of cystically dilated acini, to a final consistent staining of apocrine‐lined cysts. Zinc alpha2glycoprotein was demonstrated in 16 of 33 invasive carcinomas, 15 of which were eosinophilic on haematoxylin and eosin staining, and in one of three non‐invasive carcinomas. No staining was apparent in other non‐mammary tissues and carcinomas apart from weak reactivity of serous cells of the parotid gland. Zinc alpha2glycoprotein is, therefore, a reliable immunohistochemical marker of apocrine cell differ

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb02670.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The detailed light and electron microscopic and immunohistological features of an invasive apocrine carcinoma of the breast developing in a 78‐year‐old woman are presented. The stroma of the tumour contained non‐neoplastic lipid‐filled (foam) cells. To our knowledge, these cells have not been described before in invasive breast carcinoma. Their electron microscopic and immunohistological features confirm their histiocytic

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb02671.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

A case is reported of nodular lymphocyte predominant Hodgkin's disease arising 13 years after the removal of a lymph node from the same site which showed marked progressive transformation of germinal centres. The morphological evidence for a histogenetic relationship between the two conditions is presented and discussed.

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb02672.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Histochemical characteristics and the distribution of gastric intramucosal cysts were studied in 50 cancerous and 51 benign gastrectomy specimens. The frequency of such cysts was significantly higher in stomachs with carcinoma (70%) than in stomachs with peptic ulcer (43%) (P<0.01). Intramucosal cysts were classified into gastric type, small intestinal type, colonic type and non‐mucous type. There were significant differences in the constituent ratios of the four types of cyst between gastric carcinoma and gastric ulcer (P<0.01), as well as between intestinal type and diffuse type cancer (P<0.001). The present results reveal a close relationship between intramucosal cysts and gastric carcinoma. Cysts of small intestinal, colonic and non‐mucous types were associated with intestinal type cancer while cysts of gastric type were related to diffuse type cancer of the stom

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb02673.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

A case of pulmonary alveolar microlithiasis is presented which has been investigated using X‐ray energy spectroscopy and microscopic infrared spectroscopy. These methods gave precise details of the chemical composition of the microliths seen in this unusual disease. The pathogenesis of this disorder is discussed in the light of these finding

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb02674.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

A 68–year‐old man without previous hepatobiliary or pancreatic disease was admitted after five attacks of nausea, vomiting, abdominal pain and high fever. Laboratory investigations indicated cholestatic liver disease and pancreatitis. For 1.5 years the patient had occasionally been taking a non‐steroidal anti‐inflammatory drug, sulindac (clinoril, MSD, New York), for osteoarthritis. On suspicion of a drug‐associated disease, a rechallenge experiment was performed with sulindac. Five hours after drug administration symptoms recurred. There was a pronounced increase in serum alkaline phosphatase and amylase. A liver biopsy 3 d later showed portal tract inflammatory infiltration and abnormal interlobular bile ducts with degeneration and necrosis of the epithelium and neutrophilic infiltration of the ducts. Sulindac‐induced cholangitis has not been described previously. The pathogenetic mechanism is considered to be an immunoallergic idiosyncratic reaction to the active metabolite of sulindac absorbed by the bile duct epithelium. The lesion is apparently

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1987.tb02675.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY