摘要:

The aim of this study was to document the patterns of cytoplasmic Ig heavy and light chain expression in reactive lymphoid tissue, using single and double immunoenzymatic labelling techniques. This investigation was undertaken, firstly, to provide information on whether the normal counterparts of high grade lymphoma cells (e.g. centroblasts, immunoblasts) ever express more than one light or heavy chain (as has been noted in the past for lymphomas) and also, secondly, to seek evidence of intraclonal ‘switching’ from cytoplasmic IgM to cytoplasmic IgG expression. Paraffin embedded sections, all showing substantial reactive changes, were analysed by means of immunoperoxidase stains for the three major immunoglobulin classes (IgG, IgM and IgA), both light chain classes and J chain. In addition, double immunoenzymatic labelling techniques were used to search for cells showing simultaneous expression of kappa and lambda light chains and cells expressing mu and gamma heavy chain. Large transformed lymphocytes showing cytoplasmic Ig‐staining in the pulp and interfollicular areas often have nuclear morphology indistinguishable from germinal centre centroblasts. There was no evidence of primitive appearing IgM‐positive cells and IgG‐positive cells of more mature morphology. In addition, immunoenzymatic staining showed that cells simultaneously expressing both IgG and IgM are only rarely encountered. When such cells were detected, the morphology was not that of a blast cell, but rather of a plasma cell containing Russell bodies. Hence it is suggested that cytoplasmic IgM switching to IgG is rarely detected by immunohistological methods in reactive tissue. Double staining for kappa and lambda revealed that cells simultaneously expressing both light chain types were not detected even among cells showing the most primitive m

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1983.tb02298.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Three cases of histiocytic necrotizing lymphadenitis are reported. Two patients came from Vietnam and the third from Greece. In all cases there was infection withYersinia enterocoliticaof serogroup 9 or 3. Cervical lymph nodes were examined. Histologically, the characteristic necrosis developed in large foci of so‐called T‐associated plasma cells. Immunological analysis showed that these cells have characteristic markers of helper/inducer T cells, but do not express sheep erythrocyte receptors. The T‐associated plasma cells perished by pyknosis and were then phagocytosed and digested by macrophages, which were present in large numbers. The necrotic areas were exclusively located in hyperplastic T regions. The B‐cell system did not play a role in the reaction. T‐associated plasma cells have been renamed ‘plasmacytoid T cells’ because they contain abundant rough endo‐plasmic reticulum (‘plasmacytoid’) and show immunological features of T cells. It appears likely that plasmacytoid T cells are the counterparts of plasma cells of the B‐cell system that secrete lymphokines inst

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1983.tb02299.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Tissue from 53 non‐Hodgkin's lymphomas of high‐grade malignancy according to the Kiel classification were analysed for cellular immunological markers. In most cases studies were performed in parallel on cell suspensions and cryostat sections. Histologically, the lymphomas were classified as anaplastic centrocytic (four), centroblastic (seven), Burkitt type (three), convoluted‐cell type (five) lymphoblastic‐unclassified (10), immunoblastic (IBL) (19) and pleomorphic T‐cell type (five). Immunological phenotyping resulted in 60% B lymphomas characterized by monotypic surface membrane Ig (SmIg) and/or cytoplasmic Ig (CIg), and 23% T lymphomas with detectable E receptors; 17% of cases were non‐expressive (O‐type). Unusual SmIg‐types were noticed in some monoclonal proliferations. Gamma (γ) and μ chains occurred simultaneously in four cases; δ chain was the only heavy‐chain in one case and a heavy‐chain was absent in one case. Cases of IBL were of T‐cell type in two cases, and two other cases were non‐expressive. The cases of B‐IBL expressed CIg in 93%, but the B lymphomas other than B‐IBL only in 38%. Receptors for Fc‐IgG and C3 were expressed by all major immune phenotypes (B, T, O), but were infrequent in lymphoblastic lymphoma unclassified (O‐type). Adoption of immunological techniques to include frozen tissue studies was necessary in order to reach a conclusion regarding the

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1983.tb02300.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The ultrastructural appearance of surface epithelial cells of normal and abnormal pyloric epithelium is presented. In addition to areas of complete intestinal metaplasia (IM) an incomplete metaplasia is described which contains poorly differentiated mucous cells, goblet cells, an occasional immature absorptive cell as well as the mature mucus‐secreting surface epithelial cells of normal pyloric epithelium. This type of epithelium frequently appeared hyperplastic and showed alterations in the surface microvilli, terminal webs, plasma membranes and mucin granules. Apical vesicles, small electron‐dense bodies and larger electron‐dense multivesicular bodies were also found in the intermediate cells of incomplete IM and in the mature absorptive cells of complete IM. The intermediate cells described here may be synonymous with the sulphomucin‐secreting cells found in adenocarcinomas of the stomach and oes

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1983.tb02301.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Dudoenal biopsies from 11 patients with non‐ulcer dyspepsia were examined by electron microscopy. Chronic duodenitis with gastric metaplasia was seen in samples from four patients, and areas of normal‐looking duodenal mucosa were present in nine. The metaplastic cells were similar to those previously described in association with duodenal ulcers. They usually showed marked morphological differences from the normal duodenal absorptive cells. The microvilli were distorted and reduced in number, the glycocalyx was ill‐defined, the cytoplasm filled with mucous globules and the intercellular spaces, in some areas, were widened and contained acute and chronic inflammatory

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1983.tb02302.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

A histological study of 208 cases found a strong association between the occurrence of precancerous cervical columnar and squamous lesions. This was not, however, directly related to cellular abnormalities. The criteria for adenocarcinomain situ(AIS) and its precursor lesions were defined morphometrically. With increasing abnormalities of the columnar cells, changes of the glandular and epithelial architecture were more frequently observed. Of the 20 cases morphometrically classified as AIS, only four were initially diagnosed as such, of which three were cases of ‘pure’ AIS lacking squamous cell abnormalities. Quantifying abnormalities of columnar epithelium is an important aid in the identification and classification of

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1983.tb02303.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Eleven patients with verrucous carcinoma of the penis are presented. The age range was from 41 to 80 years with a mean of 59 years. The pathological features of these tumours are described and the aetiology of penile verrucous carcinoma is discussed.

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1983.tb02304.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The ultrastructure of stromal mononuclear cells and giant cells were studied in two cases of central giant cell granuloma of the jaws. The majority of stromal cells superficially resembled fibroblasts, but also contained intracytoplasmic myofilaments with electron dense bodies similar to smooth muscle cells. These cells were referred to as myofibroblasts. The other type of mononuclear cells resembled macrophages. Many of the myofibroblast type cells appeared close to giant cells and signs of early fusion between them could be demonstrated. It is suggested from these results that the giant cells in central giant cell granuloma form and increase in size through fusion of these cells.

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1983.tb02305.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Surgical biopsy specimens from five children with cerebellar medulloblastoma were examined for ultrastructural evidence of differentiating activity. At light microscope level, all the tumour samples appeared primitive and featureless, and could not be classified as showing either neuroblastic or spongioblastic differentiation. Using electron microscopy, the major part of each specimen examined likewise showed largely primitive and undifferentiated appearances typical of medulloblastoma. In all the cases however, scattered groups of cells and related cytoplasmic processes were found with more specific, differentiated features. Moreover, in each tumour sample, these cells and processes were found to show two morphologically distinct appearances. One cell type had ultrastructural features reminiscent of primitive neuronal morphology, and a similar appearance was found in cell perikarya forming rosettes. The other cell type showed evidence of spongioblastic differentiation, with bundles of glial‐like filaments in the cytoplasm. Cilia were also found in one case. Whilst in keeping with current views on the neuroectodermal origin of the tumour, these observations suggest that all medulloblastoma cells may have bipotential differentiating capacity, and that at least some evidence of this is likely to be found ultrastructurally, even when there is only a primitive and completely undifferentiated appearance on light microscop

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1983.tb02306.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The occurrence of intramucosal cysts in the mucosa of resected stomachs was recorded in 91 consecutive patients having synchronous focal (elevated) dysplasia and/or adenocarcinoma elsewhere in the stomach. Intramucosal cysts were classified into foveolar cysts, pyloric gland cysts, fundic gland cysts, intestinal metaplastic cysts and ciliated‐cell cysts. The most common type was intestinal metaplastic cyst, which was present in 60% of the patient

ISSN:0309-0167    

DOI:10.1111/j.1365-2559.1983.tb02307.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY