摘要:

We examined full thickness specimens of oesophageal squamous dysplasia from both cancer‐free and cancer patients using immunohistochemical labelling for cytokeratin subtypes 10/13 and 14 and for involucrin, binding studies for various lectins, and PAS/D staining before and after diastase treatment. We studied specimens from patients with oesophageal carcinoma (52 normal epithelia, and 49 with mild, 38 with moderate, and 32 with severe dysplasia), and 32 specimens from cancer‐free patients (five normal epithelia and 16 with mild and 11 with moderate dysplasia). Abnormal cytokeratin expression patterns in atypical cells, i.e. both cytokeratin 10/13 and cytokeratin 14 immunoreactivity in the same cells was detected in 41 of 99 specimens with dysplasias in cancer patients.Helix aspersaErythrina cristagalliandRobinia pseudoacaciabinding was consistently negative in atypical cells in squamous dysplasia. The non‐atypical layer of squamous dysplasia, which was morphologically indistinguishable from the corresponding layer of normal oesophageal squamous epithelium, showed abnormal involucrin expression in 39/101 specimens,Helix aspersabinding in 74/106, diastase sensitive PAS staining in 52/110,Erythrina cristagllibinding in 28/107, andRobinia pseudoacaciabinding in 16/100. There were no significant differences in the expression of these markers in dysplasia between cancer patients and cancer‐free individuals with the exception of increasedRobinia pseudoacaciabinding in the non‐atypical layer in cancer‐free patients. The results indicate that abnormal patterns of cytokeratin expression and lectin binding occur not only in atypical cells but also in non‐atypical cells in oesophageal squam

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-480.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

Sixteen cases of ductal (endometrioid) carcinoma of the prostate are presented. The tumour presents in elderly men (age range 65–87 years) with haematuria or obstructive symptoms. Serum prostate specific antigen may be normal or raised. On cytoscopy, there is often an exophytic lesion in the region of the verumontanum. Histologically, two variants are recognized: papillary and cribriform, of which there were eight cases each. Eight cases consisted of pure ductal carcinoma and seven were mixed, containing a variable proportion of micro‐acinar carcinoma.The associated micro‐acinar carcinoma had a Gleason score of at least 5. One case of carcinosarcoma with a ductal epithelial component was also included. All cases displayed positive immunohistochemical staining for prostate specific antigen and prostatic acid phosphatase and but were negative for the basal cell marker MA903. The tumour responds well to orthodox micro‐acinar carcinoma therapy and appears notably sensitive to hormonal manipulation. Follow‐up of the mixed group is restricted to a maximum of 3 years. Of the eight pure cases, five patients are still alive with survival periods of 11, 8, 7, 3 and 1 years. Three patients died of intercurrent disease of which one patient survived 12 years, having received no treatment. This tumour, therefore, can be regarded as having a good

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-483.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

Intra‐abdominal panniculitis is a tumour‐like inflammatory condition of adipose tissue. The aetiology and pathogenesis of the disease is unknown, but a number of associated diseases have been recorded. It has been customary to deal with only primary cases in the literature. This study was undertaken in order to describe the entire spectrum of the disease including primary as well as secondary cases. Eleven patients are reported, nine of which had an associated colorectal disease in direct continuity with areas of intra‐abdominal panniculitis. It is concluded that intra‐abdominal panniculitis should not be regarded as a specific nosological entity but merely a result of injury to the fat cells. Intra‐abdominal panniculitis is seen more often as a secondary local phenomenon than as a primary condition, and in both cases it is associated with considerable differential diagnostic problems. Primary diseases involved are, among others, colorectal carcinoma and diverticulosis. The aetiologic agent(s) are still unknown, but substances liberated from a damaged bowel might play a pathogen

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-486.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

The clinicopathological features and immunohistochemical findings in 14 primary sarcomas of the lung collected over a 30‐year‐period are presented. This represents one sarcoma per 550 bronchogenic carcinomas undergoing resection in this centre. The study group comprised six leiomyosarcomas, five malignant peripheral nerve sheath tumours, two haemangiopericytomas and one epithelioid haemangioendothelioma. The majority of cases occurred in men (nine males: five females), with mean age at presentation of 54 years for men and 47 years for women. All leiomyosarcomas were seen in men, whereas malignant peripheral nerve sheath tumours showed no particular sex preponderance. Leiomyosarcomas were larger tumours than malignant peripheral nerve sheath tumours, mean tumour diameter 15 cm (range 10–25 cm) compared to 9.5 cm (7–15 cm), respectively. All leiomyosarcomas were situated intraparenchymally whereas two of the five malignant peripheral nerve sheath tumours were endobronchial in site. Extrathoracic metastates were seen at death in two of the six leiomyosarcomas but not in any of the malignant peripheral nerve sheath tumours. Overall survival was 28 months although for the leiomyosarcoma/malignant peripheral nerve sheath tumour group alone survival was 8 months. Tumour grading appeared to be a more useful prognostic factor than tumour site (endobronchial/parenchymal) or tumour size. Haemangiopericytoma and epithelioid haemangioendothelioma were associated with a more favou

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-481.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

To elucidate the phenotype of the blood vessels and the expression of the growth factors involved in angiogenesis in metastatic liver cancers, we carried out an immunohistochemical study of 57 surgically resected livers with metastatic cancer. Blood vessels in the metastatic liver cancers frequently expressed von Willebrand factor (vWF),Ulex europaeusagglutinin I (UEA I)‐binding sites, α‐smooth muscle actin (α‐SMA), type IV collagen and laminin. Sinusoidal endothelial cells around the metastatic liver cancers were positive for vWF in 33.3% of the specimens examined and for UEA I in 28.1%. α‐SMA‐positive perisinusoidal cells accumulated in the vicinity of the metastatic liver cancers in 68.4% of the specimens. Type IV collagen was detected in the perisinusoidal space close to the metastatic cancers as well as distant from them (91.2%). Laminin was detected in the perisinusoidal space in only one specimen (1.8%). Tumour cells of the metastatic liver cancers were positive for vascular endothelial growth factor, basic fibroblast growth factor (bFGF), and acidic fibroblast growth factor (aFGF) in 78.9%, 38.4% and 7.0% of the specimens, respectively. Hepatocytes close to the metastatic liver cancers expressed bFGF more strongly than those distant from the metastatic liver cancers, and their expression of bFGF was more intense than that in the tumour cells. These results suggest that: (1) tumour vessels in metastatic liver cancers consist of endothelium, basement membrane and pericytes, (2) the sinusoids adjacent to tumours undergo capillarization, and (3) vascular endothelial growth factor may contribute to angiogenesis in metastatic liver cancer. Basic fibroblast growth factor may be responsible for the sinusoidal capillarization and the peritumo

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-484.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

Certain oncogenes and tumour suppressor genes are known to modulate apoptosis. To investigate whether over‐expressed bcl‐2 and abnormally stabilized p53 are associated with reduced apoptosis in paraffin sections of non‐small cell lung carcinoma, apoptotic, mitotic, and Ki‐67 labelling indices were determined and correlated with bcl‐2 and p53 immunoreactivity in 54 squamous cell carcinomas and 22 adenocarcinomas. Nineteen squamous cell carcinomas (35.2%) showed over‐expression of bcl‐2, but all 22 adenocarcinomas were bcl‐2 negative. Thirty‐seven squamous cell carcinomas (68.5%) and 13 adenocarcinomas (59.1%) showed p53 over‐expression. Apoptotic tumour cells were identified among p53 positive and bcl‐2 positive tumour cells. There was a significant linear correlation between apoptotic indices and mitotic indices. bcl‐2 over‐expression and p53 over‐expression were not associated with attenuated apoptosis, or altered mitotic or Ki‐67 labelling indices in either tumour type. Neither bcl‐2 nor p53 was of prognostic significance. These results suggest that apoptosis in non‐small cell lung carcinoma occurs independently, and is not modulated primarily by, bcl‐2 or p53. It is likely that the effects on apoptosis of bcl‐2 and p53 are countered by those of other oncogene products and/or additional

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-478.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

A multifocal lymphoepithelioma‐like carcinoma and a low‐grade B‐cell lymphoma of mucosa‐associated lymphoid tissue (MALT‐type) were found simultaneously in the stomach of a 65‐year‐old patient. Carcinoma and lymphoma were intimately associated forming complexes resembling lymphoepithelial lesions at the primary gastric site and in lymph node metastases. The two tumours had developed on a background of severe chronic‐atrophic gastritis of the mucosa of antrum and fundus. Autoantibodies to normal gastric glandular tissue could be demonstrated in the patient’s sera. Using non‐radioactivein situhybridization (ISH), Epstein‐Barr virus (EBV) sequences were detected in virtually all carcinoma cells but neither in the non‐neoplastic mucosa nor in the lymphoma. These findings suggest that a focal EBV infection occurred early in the development of the carcinoma followed by a subsequent clonal expansion of the EBV‐containing tumour cells. A neoplastic transformation in MALT‐type lymphoma is not EBV‐related but might be triggered

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-482.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

The biological behaviour of meningeal haemangiopericytomas was retrospectively studied using immunohistochemical staining with MIB1, a monoclonal antibody against the Ki‐67 antigen, a nuclear protein related to cell proliferation. Paraffin‐embedded material from 62 tumours from 40 patients were investigated. The proliferating compartment of the tumours was estimated by evaluating the MIB1 staining index, i.e. the percentage of MIB1 positive nuclei in at least 1000 counted tumour cells in representative areas. The staining index ranged from 1.24% to 39.01%. Statistical analysis revealed no significant correlation between the staining index and recurrence‐free survival (χ2 = 0.3922,P = 0.5311). Long‐term observation (>100 months), however, revealed a tendency to longer survival in the group with a staining index less than 5%. According to our results, the MIB1 staining index does not contribute to the accuracy of predicting the clinical outcome of meningeal haem

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-479.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

It is well known that chemotherapy induces cytomorphological changes in neoplastic and non‐neoplastic tissue. Thirty‐one stage III breast‐carcinoma patients, treated with both pre‐operative chemotherapy and mastectomy, were evaluated to define the effects of systemic chemotherapeutic agents in tumours, non‐neoplastic breast tissue, and lymph nodes. Histological changes were compared with those observed in patients who had been treated by surgery alone. Cytoplasmic vacuolization was the most striking change in the tumour cells (59%). Chemotherapy was especially effective in the terminal duct lobular unit in non‐neoplastic breast tissue. Lobular atrophy was observed in 20 (65%) cases, and lobular cellular atypia was seen in 16 (52%). The rate of ductal cellular atypia (42%) was not different from the control group. The most important changes seen in the non‐neoplastic stromal component were fibrosis and hyalinization. These were found in 31 out of 727 evaluated lymph nodes. In serial sections, metastatic deposits were seen in or around these fibrotic or hyalinized areas. Chemotherapy is widely used in the treatment of early and locally advanced breast carcinomas. Familaritiy with chemotherapy induced changes in breast tissue and lymph nodes have considerable importance in the accurate interpretation of th

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-485.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

Two new cases of human pulmonary dirofilariasis, in a 66‐year‐old woman and a 69‐year‐old man, respectively, both living in the Po valley, are described. They represent the 7th and 8th cases reported in Italy and seem to be ascribable toDirofilaria repens, even though the poor state of preservation of the parasites did not allow the morphology of the species involved to be clearly defined. In both cases, the false clinical diagnosis of lung cancer was based on the accidental radiological discovery of a coin lesion, the nematodes being located in a pulmonary artery producing a small, roundish

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-487.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY