ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-498.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

We describe the clinicopathological features of gastrointestinal autonomic nerve tumours in nine patients where the diagnosis was confirmed by electronmicroscopy. Most patients presented with abdominal pain. At laparotomy, large intra‐abdominal tumour masses were found which tended to be cystic and haemorrhagic. The predominant histological patterns were nests, sheets and fascicles of spindle and epithelioid cells. Immunohistochemistry showed positive staining for neuron specific enolase (9/9), PGP 9.5 (9/9), NKI/C3 (7/9), vimentin (7/9), α‐smooth muscle actin (5/9), vasoactive intestinal peptide (3/9) and CD34/QBend10 (2/9). Grimelius staining was positive in two of nine cases. All tumours were negative for CAM 5.2, chromogranin, synaptophysin, Leu 7, neurofilament protein, muscle‐specific actin (HHF‐35) and desmin (D33). Ultrastructural examination showed cellular processes and dense‐core granules in all cases. Three tumours had microtubules and/or intermediate filaments, particularly in cell processes. Skeinoid fibres were seen in three cases. No convincing synapses or small (synaptic‐type) vesicles were identified. There was no evidence of epithelial, smooth muscle or nerve sheath differentiation. Two patients died due to tumour, two died of unknown causes and the remainder are alive 2–44 months after presentation. Four of the five survivors have recurrent/residual intra‐abdominal tumour. So‐called gastrointestinal autonomic nerve tumours are apparently slow‐growing malignant tumours showing neuronal differentiation. Four cases arose in the mesentery/retroperitoneum or omentum rather than bowel wall and therefore a more appropriate nomenclature might be intra‐abdominal stromal tumour with ne

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-502.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

We examined apoptosis in 33 gastric carcinomas using the terminal deoxynucleotydil transferase mediated dUTP‐digoxigenin nick end labelling technique (TUNEL). Of the tumours, nine were well‐differentiated, 13 moderately differentiated and 11 poorly differentiated. In addition, we also analysed MIB‐1, a cell proliferation antigen. Morphologically, apoptotic tumour cells were more frequently observed in well‐differentiated tumours. In addition, apoptotic signals of the TUNEL method were seen even in the nuclei of tumour cells which did not show apoptosis. The nick end labelling index was 51.0 ± 26.3 in the well‐differentiated and moderately differentiated tumours and 28.0 ± 18.8 in poorly differentiated tumours. The mean of apoptotic body index and nick end labelling index were both significantly higher in well‐differentiated and moderately differentiated tumours than in the poorly differentiated type (P < 0.0001,P = 0.008). The MIB‐1 labelling index and higher in poorly differentiated tumours than in the well‐differentiated or moderately differentiated tumours, and labelled cells were more numerous in the superficial region than in the middle and deep regions of tumours. No apparent correlation was found between the nick end labelling index and the MIB‐1 labelling index. The high number of apoptotic cells (the high Nick end labelling index) and low proliferation potentiality (the low MIB‐1 labelling index) in well‐differentiated gastric carcinomas may thus be related to their natu

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-492.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

The expression of the cytokeratins (CK) 1, 5, 6, 7, 10, 13 and 14 was studied immunohistochemically in gastric biopsies from both the antrum and the body of 70 patients. Normal gastric foveolar epithelium (9 cases)Helicobacter pylorigastritis (23) and intestinal metaplasia (38) were examined. Positive staining results for CK 1, 5, 10 and 14 were not observed using the 34βE12 antibody. With antibodies to CK 5/6, 7 and 13 some, but not all cases, were immunoreactive. Predominantly positive staining included less than 10% of the cells and was always restricted to the tips and the juxtaluminal areas of the foveolae. No difference was seen between the antrum and the body. Comparing normal gastric mucosa with gastritis and intestinal metaplasia, cases positive for CK 5/6 were observed less frequently in intestinal metaplasia types II and III compared to the other groups. CK 7 was expressed exclusively in intestinal metaplasia. CK 13 was seen in all groups of specimens. Thus, cytokeratins typical for ductal structures (CK 7) and squamous epithelia (CK 5/6, CK 13) can be regarded as an inconstant, but not unusual observation in the gastric mucosa. Their expression may be controlled by both differentiation‐related as well as environmental facto

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-496.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

Expression of β‐catenin was investigated in normal breast tissue and 66 breast carcinomas in conjunction with expression of epithelial cadherin (E‐CD) and α‐catenin. In normal mammary ducts and acini, intense β‐catenin immunoreactivity was present at the basolateral surfaces of luminal epithelium and weak immunoreactivity was observed at the lateral borders of myoepithelial cells. No β‐catenin was revealed at the myoepithelial basal surface. The intercellular expression of β‐catenin, as well as of E‐CD and α‐catenin, was also observed in carcinoma tissues with varying staining intensity. Almost all of 10 intraductal carcinomas and approximately 70% of 41 invasive ductal carcinomas expressed the three molecules at the same level as in normal glands, whereas approximately 80% of 13 invasive lobular carcinomas showed severe deficiency of them. Two lobular carcinomasin situshowed complete absence of all of the proteins. Some of these findings were confirmed biochemically by immunoblotting analysis. In invasive ductal carcinomas, α‐catenin was reduced more frequently in diffuse than in solid type tumours, whereas the level of expression of β‐catenin and E‐CD was unchanged between them. No correlation was present between reduced expression of the adhesion molecu

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-499.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-510-2.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

The aim of this study was to evaluate the utility of a new commercially available antibody to thrombomodulin as an endothelial marker in formalin‐fixed paraffin‐embedded tissue. The expression of thrombomodulin in a variety of 50 vascular and lymphatic neoplasms and malformations was compared to the expression of von Willebrand factor, QBend 10 (CD34) and JC70 (CD31). We showed that thrombomodulin was the best marker of lymphatic endothelium and also stained a higher percentage of malignant vasoformative tumours when compared to the other markers. We recommend the assessment of thrombomodulin expression in the differential diagnosis of malignant vasoformative neoplasms and in the detection of lymphatic endothelium for evidence of tumour permeat

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-500.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

The aim of this study was to examine the immunohistochemical expression of p53 and bcl‐2 in Kaposi’s sarcoma and relate this with proliferation index (as measured by MIB‐1 staining) and clinicopathological subtypes. Twenty formalin‐fixed, paraffin‐embedded cases of Kaposi’s sarcoma were stained with commercially available antibodies to p53, bcl‐2 and MIB‐1, after pressure cooking antigen retrieval. All cases were strongly positive for bcl‐2 with the majority containing more than 75% positive cells. In comparison, p53 expression was less striking. Eleven cases contained less than 24% (+1) of cells staining positively. Only two cases showed greater than 75% of positive cells, and both of these latter two lesions had metastasized. The MIB‐1 staining in all cases of Kaposi’s sarcoma was strongly positive, irrespective of clinicopathological type, in keeping with the highly proliferative nature of this lesion. Thus, we have demonstrated uniformly increased expression of bcl‐2 protein in Kaposi’s sarcoma irrespective of clinicopathological subtype and MIB‐1 staining, while p53 expression is relatively less common, except in those cases which have metastasized. This may help identify those cases that will behave in a more aggressive manner. However, more cases need to

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-493.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

Desmoplastic malignant melanoma often arises in sun damaged skin of the head and neck and shows frequent neurotropism. Although metastatic melanoma frequently involves the parotid, direct spread to the parotid has been rarely reported. We evaluated five cases of desmoplastic malignant melanoma involving the parotid gland with clinical and pathological evidence of precursor cutaneous lesions in four of the five cases. The parotid involvement in four cases was tumoural, and three of these were not clinically suspected to be melanoma. The histological appearance in all five cases was that of a sarcomatoid tumour. Immunohistochemistry and electronmicroscopy performed on three of the cases showed only evidence of schwannian differentiation: the tumour cells were positive for S‐100 protein and vimentin, and negative for cytokeratin and HMB‐45. Electronmicroscopy showed no evidence of melanogenesis. All five tumours showed histological evidence of prominent neurotropism with one case demonstrating extension from overlying skin along cutaneous nerves to the superficial parotid. Thus, desmoplastic malignant melanoma may involve the parotid by neurotropic spread and can be pathologically indistinguishable from malignant schwannoma, a diagnosis which may be made erroneously in the absence of clinical informat

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-497.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

We report a patient with fibrinogen storage disease in which there was proliferation of normal‐sized peroxisomes in the hepatocytes. This phenomenon has previously been described in several acquired liver diseases. We believe that this is an adaptation response due to decreased microsomal isoenzyme activity as a result of the excess accumulation of fibrinogen in the endoplasmic reticulu

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.d01-495.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY