摘要:

We have compared the clinical and histological features of 149 complete moles with 146 triploid partial moles and 107 diploid non‐molar hydropic abortions initially registered as moles for human chorionic gonadotrophin (hCG) follow‐up. Forty‐one patients with complete moles, five with partial moles and one with hydropic abortion received chemotherapy for hCG elevations interpreted as persistent trophoblastic disease. Complete moles were aborted or were evacuated significantly earlier than partial moles (means of 12.1 and 15.4 weeks;P <0.001) and hydropic abortions significantly earlier than complete moles (mean 10.7 weeks;P<0.005). The means of the highest recorded hCG were higher in complete moles (184 056 i.v.) than in partial moles (66 259 i.v.) and hydropic abortion (7942 i.v.). When hCG became normal without chemotherapy, this occurred earlier in patients with hydropic abortion than in those with partial moles (means of 46.7 days and 62.8 days;P<0.001) and earlier in partial moles than in complete moles (mean 78.3 days;P<0.005). The incidence of partial moles was comparable throughout fertile years but rose to 1.9 times the average after 40 years. Complete moles were commoner between 14 and 25 years and after 35 years, reaching 4.8 times the average after 40 years. Hydropic abortions were rare before 25 years and increased with age to 12 times the average after 40 years. Stromal karyorrhexis and shape of villi, before they become hydropic, discriminate well between complete and partial mole. Hydrops increased and vascularity decreased with molar age and the presence of non‐hydropic villi or vessels did not discriminate between partial mole and the younger complete moles evacu

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.247295.x

出版商:Blackwell Science    

年代:1996

数据来源: WILEY

摘要:

We previously described nine cases of angiocentric lymphoma of a possible natural killer (NK)‐cell lineage with a surface CD3− CD56+ phenotype occurring in sites other than the upper and lower respiratory tract. This study was performed to investigate the association of Epstein‐Barr virus (EBV) with these lymphomas, using the polymerase chain reaction (PCR) for the presence of EBV‐DNA, in situ hybridization (ISH) for EBV‐encoded small RNAs (EBERs) and immunohistology for EBV‐determined nuclear antigen‐2 (EBNA‐2) and latent membrane protein‐1 (LMP‐1) in paraffin sections. PCR and ISH produced almost identical results, and EBERs were identified in the nuclei of the lymphoma cells of three cases, two of which exhibited LMP‐1 in the cytoplasm of tumour cells without EBNA‐2 expression. Molecular genetic analysis revealed EBV to be incorporated into these three EBER‐positive cases either clonally or biclonally. It was revealed by re‐evaluation of their morphology with the established EBV status on each case that, in contrast to the rather variable and irregular cellular composition of the EBV‐ positive tumours, the EBV‐negative tumours stood out because of their remarkably uniform ‘blastoid’ appearance, and could be grouped as blastic NK‐cell lymphoma. The relationship of the EBV‐positive cases with nasal

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.278324.x

出版商:Blackwell Science    

年代:1996

数据来源: WILEY

摘要:

Recent reports demonstrated the presence of Epstein‐Barr virus (EBV) in about 10% of gastric carcinoma cases, particularly in Asian populations. We carried out a retrospective assessment of the detection rate of EBV gene products in 59 cases of gastric carcinoma of various histological subtypes. In situ hybridization using non‐isotopic EBER and BHLF1 oligoprobes, and immunohistochemistry using antibodies to latent membrane protein 1 (LMP‐1) were applied to paraffin‐embedded sections. Tumour cells in five out of 59 cases (8.5%) were found to be EBER positive by in situ hybridization, but no staining was observed with LMP‐1 antibodies. Four EBER positive cases were lymphoepithelioma‐like carcinomas and one case was a well differentiated adenocarcinoma, suggesting a stronger association with the former subtype. Among the four EBER positive lymphoepithelioma‐like carcinomas, BHLF1 transcripts were expressed in one case in a few tumour cells, indicating the possible activation of a lytic cycle. In nine cases (including three EBER positive cases) a few scattered EBV‐infected lymphocytes were seen in the normal mucosa but we were unable to detect any EBER positive normal epithelial cells. Our results show that, in a French population, the incidence of EBV‐associated gastric carcinoma is similar to that in other geographic areas. The clinical implications of these findings, howeve

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.287333.x

出版商:Blackwell Science    

年代:1996

数据来源: WILEY

摘要:

An increased incidence of non‐Hodgkin‘s lymphoma is seen in patients with immunodeficiency from any cause. The majority of these are high grade B‐cell lymphoma and most are associated with the Epstein‐Barr virus (EBV). In post‐transplant lymphoma/lymphoproliferative disorders the tumour may regress following reduction of immunosuppression but in AIDS the lymphomas show a characteristic aggressive course and poor prognosis. We describe low grade B‐cell gastric lymphoma of mucosa associated lymphoid tissue (MALT) in three immunocompromised patients (two post‐transplant, one HIV positive). In each case, the tumour showed classical morphological features of gastric MALT lymphoma and was not associated with EBV.Helicobacter pyloriwas identified in each case. Clinical follow‐up suggests that the behaviour in these tumours is similar to that seen in MALT lymphomas in immunocompetent patients and not typical of the lymphomas usually associated with immunosuppression. Although the finding of MALT lymphoma in immunosuppressed patients might be coincidental, the association of some MALT lymphomas with autoimmune disease suggests that dysregulation of the immune system might play a role in the pathogenesis o

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.292338.x

出版商:Blackwell Science    

年代:1996

数据来源: WILEY

摘要:

The expression of bcl‐2 protein was studied by immunohistochemistry using a monoclonal antibody in 158 cases of transitional cell bladder tumours. The bcl‐2 protein was expressed in basal cells in normal transitional epithelium and 68% of the transitional cell tumours showed bcl‐2 positivity in the basal cells. The expression of bcl‐2 in basal cells was positively correlated to over‐expression of epidermal growth factor receptor in tumour cells. The expression of bcl‐2 in non‐basal cells was weak in 20% and strong in an additional 13% of tumours. The expression of bcl‐2 in non‐basal cells was positively correlated to T‐category, M‐category, grade, papillary status, DNA ploidy, S phase fraction, mitotic index, nuclear area and over‐expression of epidermal growth factor receptor. Recurrence‐free interval of Ta‐T1 tumours was related to bcl‐2 positivity in non‐basal cells. Tumours with bcl‐2 positive non‐basal cells had an unfavourable prognosis but, in multivariate analysis, expression of bcl‐

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.276322.x

出版商:Blackwell Science    

年代:1996

数据来源: WILEY

摘要:

bcl‐2 is a proto‐oncogene which inhibits apoptosis. In contrast, p53 tumour suppressor gene is known to induce apoptosis. In this study we analysed immunohistochemically 63 mesenchymal tumours for the expression of bcl‐2 and p53 proteins with a special emphasis on muscle‐derived tumours. bcl‐2 was expressed in all seven rhabdomyosarcomas and in five out of seven leiomyosarcomas. In benign muscle tumours, bcl‐2 expression was found in all four epithelioid leiomyomas and in six out of 14 leiomyomas. In non‐neoplastic muscle cells, occasional weak bcl‐2 positivity was found in muscle cells of vascular walls and myometrium. In mesenchymal tumours of other lineages, bcl‐2 positivity was only found in four out of 12 malignant fibrous histiocytomas and in one out of three liposarcomas. p53 positivity was found in 14 tumours, 13 of which were sarcomas. No association was found between p53 and bcl‐2 positivity. Our results suggest that bcl‐2 expression is activated significantly more often in muscle derived tumours than in mesenchymal tumours of other lineages. Our results also suggest that p53 status does not directly affect the expression

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.288334.x

出版商:Blackwell Science    

年代:1996

数据来源: WILEY

摘要:

Vascular endothelial cells are antigenically heterogeneous and therefore it has been recommended that a range of immunohistochemical markers is employed to show the presence of cells of endothelial origin in surgical pathology. In this study we applied three monoclonal antibodies—to factor VIII‐related antigen, JC70 (CD31), QBend 10 (CD34)—and Ulex europaeus agglutinin type 1 lectin (UEA‐1), to six consecutive cases of left atrial myxoma. We found that JC70 and QBend 10 consistently stained myxoma cells in all their different growth patterns contrasting with factor VIII‐related antigen expression and UEA‐1 binding which were restricted to areas which showed morphological evidence of vascular differentiation. These findings suggest that the constituent cells of atrial myxomas show more widespread endothelial differentiation than has previously been recognized and that differences in immunohistochemical staining may reflect the maturation status of

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.271316.x

出版商:Blackwell Science    

年代:1996

数据来源: WILEY

摘要:

The cervices of 300 hysterectomy specimens have been examined for the presence of abnormal nerves morphologically resembling traumatic neuromas. These were present in 165 uteri (55%) overall but in only two of the 48 nulliparous patients (4%), indicating a probable causation by parturition. The microneuromas could be graded. The larger, more complex lesions were associated with more extensive involvement of the cervix but not with increased parity.

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.275321.x

出版商:Blackwell Science    

年代:1996

数据来源: WILEY

摘要:

Papillary endothelial hyperplasia is an exuberant predominantly intravascular endothelial proliferation which may simulate angiosarcoma both clinically and histopathologically. This report describes what, to the best of our knowledge, is a unique case of papillary endothelial hyperplasia within para‐ovarian and paratubal veins in relation to a multicystic mesothelial and haemorrhagic ovarian mass. Papillary endothelial hyperplasia should be included in the differential diagnosis of vascular lesions in this sit

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.274313.x

出版商:Blackwell Science    

年代:1996

数据来源: WILEY

摘要:

Oestrogen receptors and progesterone receptors were immunolocalized in 19 patients with benign prostatic hyperplasia and in 26 patients with prostatic carcinoma. Immunohistochemistry was performed on tissue that had been fixed in 8% paraformaldehyde and then paraffin‐embedded, using microwave irradiation for antigen retrieval. Oestrogen receptor expression was observed exclusively in the stromal cells of six out of 26 (23%) patients with prostatic carcinoma, but in none of the cells of patients with benign prostatic hyperplasia. Progesterone receptor expression was detected in 16 of 19 (84%) and 17 of 19 (89%) of the epithelial cells and stromal cells of patients with benign prostatic hyperplasia, respectively. In patients with prostatic carcinoma, progesterone receptor immunoreactivity was observed in 12 of 20 (46%) and 20 of 26 (77%) of the carcinoma and stromal cells of prostatic carcinoma, respectively. The ratio of epithelial cells with progesterone receptor immunoreactivity corresponded well with that of stromal cells with immunoreactivity in patients with benign prostatic hyperplasia. However, the ratio of stromal cells with progesterone receptor immunoreactivity was much higher than that in carcinoma cells in patients with prostatic carcinoma. Immunolocalization patterns or the ratio of the cells with progesterone receptor immunoreactivity did not significantly correlate with histological differentiation or patient’s age in carcinoma cases. However, patients with advanced surgical stages of disease demonstrated a significantly smaller number of carcinoma and stromal cells with progesterone immunoreactivity in patients with prostatic carcinoma. These results suggest that oestrogens do not have a direct effect on the biological behaviour of benign prostatic hyperplasia and prostatic carcinoma, but that progesterone appears to play a role in the pathogenesis of benign prostatic hyperplasia and prostatic carcin

ISSN:0309-0167    

DOI:10.1046/j.1365-2559.1996.280326.x

出版商:Blackwell Science    

年代:1996

数据来源: WILEY