摘要:

AbstractPulmonary interstitial macrophages (IM) were isolated from rat lungs by an Fcγ receptor‐based affinity technique coupled with multiparameter flow cytometry. Single cell suspensions obtained by collagenase digestion of extensively perfused and lavaged lungs were applied to carpets of opsonized sheep red blood cells (SRBC‐IgG) bound to plastic tissue culture flasks. At 0‐4°, optimal binding of lung cells occurred within 60 min at plating densities of 1‐2 × 106lung cells/cm2when the SRBC substrate was opsonized with 10 μg/ml anti‐SRBC IgG. Nonadherent cells were removed by gently rinsing the plates and adherent cells were recovered by lysing the SRBC‐IgG substrata. By light microscopy, the mixture of adherent cells was comprised of mononuclear cells (~ 54%), many of which appeared to be macrophages, lymphocytes (~20%), polymorphonuclear leukocytes (~15%), plasma cells (~8%), eosinophils (~2%), and mast cells (~0.5%). The cells which adhered to the SRBC‐IgG monolayers were further resolved into subpopulations by multiparameter flow cytometry and sorted according to their electro‐optical characteristics. One subpopulation appeared morphologically to be macrophages, and>90% of these cells readily phagocytized SRBC‐IgG in vitro. Peroxidase staining of this population was minimal, indicating that these cells were not blood monocytes (BM). Using a method by which alveolar macrophages (AM) were prelabeled with SRBC‐IgG in situ, we demonstrated that alveolar macrophages constituted only ~5% of the total adherent cell population. We concluded from these observations that the macrophage population harvested in this manner were neither BM nor AM, but, rather, were harvested from the lung's interstitial compartment. Flow cytometric analyses indicated that the IM exhibited electro‐optical characteristics intermediate between those of BM and AM, which is consistent with the concept of the lung's interstitium as a maturation compartment for the BM prior to migration into the alveolar compartment. However, the IM more closely resembled the BM than the AM, indicating that if the IM is in fact a precursor to the AM population, substantial maturation or differentiation must occur subsequent to its migration into the alveolar compartment. This isolation technique will be useful for harvesting highly purified IM for in vitro investigations.

ISSN:0741-5400    

DOI:10.1002/jlb.43.1.80

出版商:Wiley    

年代:1988

数据来源: WILEY

摘要:

AbstractThis review describes current understanding of the role of cells of the mononuclear phagocyte system in the development of acquired immunodeficiency syndrome (AIDS). Monocyte‐macrophage like cells have been shown to harbor human immunodeficiency virus type 1 (HIV‐I) infection in both peripheral blood and bone marrow as well as in target organs such as brain, lungs, lymph nodes, and skin. Mononuclear phagocytes that have been infected by HIV‐I do not undergo significant cytopathic changes, suggesting that they may be important viral reservoirs. These and related cells may also promote the slow, persistent nature of HIV‐I infections by escaping host immunologic surveillance mechanisms. There is evidence that HIV‐I infections in monocytes and premonocytes can initially be latent but progress to an active viral replication state upon differentiation and/or maturation. Functional abnormalities in the mononuclear phagocyte system following infection by HIV‐I have also been described, and these may be partially responsible for the severe immunosuppression characteristic of AIDS and AIDS‐related disorders. These defects may be mediated by quantitative and qualitative changes in the T‐helper population. Although the role of mononuclear phagocytes as viral reservoirs and as mediators of immunosuppression is still largely speculative, increasing evidence suggests that these cells influence the course of HIV‐I infections.

ISSN:0741-5400    

DOI:10.1002/jlb.43.1.91

出版商:Wiley    

年代:1988

数据来源: WILEY

ISSN:0741-5400    

DOI:10.1002/jlb.43.1.i

出版商:Wiley    

年代:1988

数据来源: WILEY