摘要:

Pseudomonas aeruginosaexhibit one of two flagella types: a homogeneousbtype, with molecular weight of 53 000, or a heterogeneousatype (subtypesa0,a1,a2,a3, anda4), with molecular weights ranging from 45 000 to 52 000.Pseudomonas aeruginosaflagellar antiserum was shown to promote uptake of radiolabeled bacteria by mouse polymorphonuclear leukocytes. Bacteria were detected directly associated with washed leukocytes and visualized, by electron microscopy, internalized in polymorphonuclear leukocytes. Phagocytosis was specific for the flagella type (aorb) in that homologous flagella serum enhanced uptake three to four times greater than heterologous serum or normal rabbit serum. Ana-type antiserum was shown to enhance phagocytosis of four differenta-type strains with varying subantigen types, indicating the presence of a common cross-reactivea0antigen in this flagella type. Phagocytic killing of internalized bacteria was not seen with the addition of only flagellar antiserum.Key words: flagella,Pseudomonas aeruginosa, phagocytosis, opsonic, polymorphonuclear leukocytes.

ISSN:0008-4166    

DOI:10.1139/m89-148

出版商:NRC Research Press    

年代:1989

数据来源: NRC