摘要:

An ELISA for the detection of porcine pepsin has been developed. Porcine pepsin was detected using this assay at concentrations of ≥1 μg ml‐1. Other cheese rennetting agents, such as bovine rennet orMucor mieheirennet did not cross‐react in the assay either separately or in mixtures. The ELISA was used for the determination of porcine pepsin at concentrations of 1% (10 μg ml‐1) and over in combination with bovine rennet/M.mieheirennet mixtures. The effects on the assay of samples which had been subjected to a pH range between 5.0 and 7.0 (which may be encountered during the storage of rennet mixtures and in cheese making procedures), were tested. Similarly pre‐incubation of porcine pepsin in the presence of NaCl up to 10%, conditions encountered in certain salty cheese preparations, had no subsequent effect on the sensitivity of the assay when such samples were tested. Milk proteins showed no cross‐reactivity with the anti‐porcine pepsin and increasing concentrations of casein to 75 mg ml‐1caused no significant inhibition

ISSN:0954-0105    

DOI:10.1080/09540109409354816

出版商:Taylor & Francis Group    

年代:1994

数据来源: Taylor

摘要:

The food industry must provide consumers with products which are innocuous, genuine, desirable and nutritive. To assure consumers that food has these characteristics, it is convenient to have appropriate controls of their hygiene state and to detect fraudulent practices, both of which are possible using adequate analytical techniques. However, the analysis of food has its own limitations such as specificity, reproducibility, sensitivity, economy, ease of use and standardization. Immunological techniques have objective advantages which merit their development and further use in the food industry. It has been widely demonstrated that immunological methods can be used in the food industry as reliable analytical techniques. Antibody‐based analytical methods for determining meat species and detecting adulteration of milk have been developed. We review the efforts made to develop immunological reactions and techniques to detect fraudulent replacements of foods. The selection of antigens as immunogens, the development of various polyclonal and monoclonal antibodies, and the selection of immunological techniques from immunodiffusion and immunoelectrophoresis to ELISA are described. The development of recombinant antibodies as an alternative for replacement or substitution of previously developed polyclonal or monoclonal antibody reagents will also be considered.

ISSN:0954-0105    

DOI:10.1080/09540109409354817

出版商:Taylor & Francis Group    

年代:1994

数据来源: Taylor

摘要:

A sandwich ELISA has been developed for the detection of defined amounts of goats’ milk (1–100%) in ewes’ milk and cheese. Polyclonal antibodies were raised in rabbits against goat caseins (GC). The resultant antibodies were affinity purified by immunoadsorption of the crude antiserum on to columns containing immobilized cow, ewe and goat caseins, followed by elution of the goats’ milk‐specific antibodies (anti‐GC) from the column containing the goat caseins. The anti‐GC antibodies bound to the wells of a microtiter plate were used to capture the goats’ caseins from milk or cheese mixtures. Further immunorecognition of the captured proteins was achieved with the same antibodies conjugated to biotin. ExtrAvidin‐peroxidase was used to detect biotinylated antibodies bound to their specific antigens. Subsequent enzymic conversion of the substrate gave clear absorbance differences when assaying mixtures of ewes’ milk and cheese containing variable amounts of goats’ milk.

ISSN:0954-0105    

DOI:10.1080/09540109409354818

出版商:Taylor & Francis Group    

年代:1994

数据来源: Taylor

摘要:

An indirect ELISA has been developed successfully for the detection of defined amounts of goats’ milk (1–100%) in ewes’ milk. The assay uses polyclonal antibodies against goats’ whey proteins (GWP) raised in rabbits. The anti‐GWP antibodies were recovered from the crude antiserum by immunoadsorption and elution from a column containing immobilized GWP. The anti‐GWP antibodies were biotinylated and rendered goats’ milk‐specific by mixing them with lyophilized cows’ and ewes’ whey proteins. ExtrAvidin‐peroxidase was used to detect the specific anti‐GWP antibodies bound to goats’ milk proteins immobilized on 96‐well plates. Subsequent enzymic conversion of substrate resulted in discernible differences in optical density between mixtures of ewes’ milk containing variable amounts of goats’ milk.

ISSN:0954-0105    

DOI:10.1080/09540109409354819

出版商:Taylor & Francis Group    

年代:1994

数据来源: Taylor

ISSN:0954-0105    

DOI:10.1080/09540109409354805

出版商:Taylor & Francis Group    

年代:1994

数据来源: Taylor