摘要:

The levels of fructose, acid phosphatase, citric acid, zinc, maltase, testosterone, prostaglandin E, putrescine, spermidine and spermine were compared in ejaculates collected by masturbation and after coitus in the same individuals. Coitus was associated with a significantly larger semen volume and increased concentrations and total amounts of prostaglandin E and poly‐amines in the ejaculate. In contrast, the concentrations of the conventional glandular parameters remained relatively unaffected, although a slight dilution of the seminal vesicle contribution to the ejaculate occurred after coitus. In the majority of cases, ejaculates provided by masturbation were significantly enriched in spermatozoa, but the total contributions under both conditions remained the same. It is concluded that the assessment of semen quality in male individuals using the conventional parameters of accessory gland function can be made with ejaculates provided by masturbation or coitu

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00902.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Human ejaculates were divided into 3 groups (I, II and III) according to the extent of their coagulation and the levels of free choline and cholinesterase were determined together with seminal vesicular N‐acetylamino sugar and prostatic (zinc) marker components. Coagulation was determined as the percent coagulum (CG) at 5 min after ejaculation. The mean (± SEM) values for CG at 5 min in groups I, II and III were 6 ± 2, 43 ± 4 and 79 ± 2, respectively. The time for 100% liquefaction in the 3 groups were 4.7 ± 0.8, 12.9 ± 1.4 and 19.1 ± 2.1 min, respectively. Group III had significantly higher levels of choline and cholinesterase activity than groups I and II. The choline level was correlated significantly (r = 0.58) with the concentration of N‐acetylamino sugar, but there was no correlation with the level of zinc. Evaluation of the level of choline in prostatic fluid, in semen from azoospermic men and in ejaculates with different CG values suggested that the level of choline may provide valuable information about activity of the seminal vesicles. Release of choline in group I was only 33 and 50% of that groups III and II, respectively. 60% of the total release of choline occurred during the liquefaction phase. The mean activity of cholinesterase in the prostate was only 27% lower than that found in seminal plasma. The liquefaction time and the concentration of choline, N‐acetylamino sugar and zinc decreased significantly in ejaculates after 2 days o

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00903.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Testicular vascular resistance (TVR) was estimated 1, 2, 4 and 8 h after unilteral intratesticular injection of 1 or 10 ng of an LRH‐agonist (A). The lower dose had no effect on plasma levels of testosterone or LH, but there was a significant increase in TVR 2 h after injection when compared to the vehicle‐injected contralateral testis. Injection of 10 ng LRH‐A induced an increase in plasma levels of LH at 1 and 2 h after treatment and in the plasma levels of testosterone at 1, 2 and 4 h. In the same rats there was a decrease in TVR at 2, 4 and 8 h after LRH‐A treatment compared to the contralateral testis. It is concluded that LRH‐A can have both stimulatory and inhibitory effects on TVR in the rat and that the direction of this effect may be related to the ambient le

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00904.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Sertoli cell monolayers were prepared from 30‐day‐old rat testes and cultured for 7 days to eliminate contaminant germ cells. Some of these monolayers were co‐cultured with a spermatogenic cell preparation enriched in pachytene spermatocytes and round spermatids (fraction 3 from a Percoll gradient), isolated from 30‐day‐old rat testes. After co‐culture for 4 to 48 h, germ cells were removed. RNA synthetic activity in rat Sertoli cell cultures alone was 216 800 ± 66 480 dpm [3]uridine.2h−1.106cells−1(mean ± SD) compared to 98 390 ± 23 595 in rat Sertoli cells which had been co‐cultured with germ cells of fraction 3 for 24 h (P<0.01). By contrast, RNA synthesis in Sertoli cell monolayers prepared from immature pigs were unaffected by co‐culture with rat germ cells. A similar inhibitory effect of germ cells was observed in rat Sertoli cells stimulated with FSH or testosterone. Culture medium, conditioned by 20 h culture of a fresh preparation of rat spermatogenic cells of fraction 3, was active in inducing the inhibitory effect on RNA synthesis in rat Sertoli cells. Co‐culture of rat Sertoli cells with germ cells of this fraction also decreased the incorporative of [3H]thymidine into DNA in rat Sertoli cells, from 9061 ± 3339 to 4766 ± 526 dpm.2h−1.106cells−1(P<0.01), but no such change was found in pig Sertoli cells. A different spermatogenic cell preparation, partially deprived of pachytene spermatocytes (fraction 5), stimulated rat Sertoli cell DNA synthesis (Sertoli alone 7833 ± 2550, Sertoli cells which had been in co‐culture with germ cells of fraction 5, 13 300 ± 2279 dpm.2h−1.106cells−1,P<0.05). These inhibitory actions of some germ cells on Sertoli cells were observed together with the previously reported simultaneous stimulatory effect of Sertoli cells on germ cells. These Sertoli cell‐germ cell interactions of detected in culture may represent

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00905.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Dipeptidyl peptidases (DPP) I‐IV were analysed in homogenates of bovine reproductive organs as well as in seminal vesicle secretions and seminal plasma. The presence of various molecular forms of these enzymes was studied by fractionation using gel filtration, anion exchange chromatography and chromatofocusing. The eluting enzymes were pooled, and their biochemical properties were briefly characterized. The histochemical localization of DPP II and IV was carried out with the most active tissues. DPP I and III were absent from seminal plasma, but their highest activity was found in the epididymis and increased during sexual maturation. DPP II was found mainly in a single molecular form and displayed a wide distribution in the reproductive organs. Its activity in seminal plasma may be derived from various organs, although the major sources are probably the apical activity in the epididymis, ampulla and seminal vesicle. DPP IV activity was high in the cauda epididymis, and ampulla, and in the seminal vesicles and their secretions. The high activity of DPP IV in seminal plasma appeared to derive from these organs, which showed a strong apical reaction of the epithelial lining. In seminal vesicles the enzyme was mainly secreted attached to membrane particles called vesiculosome

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00906.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

This study characterizes factors present in uterine fluid from oestrous buffalos which induce the acrosome reaction in buffalo spermatozoa. Characterization was performed by dialysis, heat treatment (90°C, 30 min) and deproteinization of uterine fluid. The % motility and viability of sperm was maintained better in unfractionated uterine fluid than in dialysed fluid. Heating and deproteinization of the dialysed uterine fluid significantly reduced the % motility and viability of sperm. The percentage of sperm showing different stages of the acrosome reaction (swelling, and vesiculation) was significantly higher for sperm incubated in dialysed than in neat uterine fluid. There was no significant difference in the % of sperm showing acrosome shedding in both dialysed and neat uterine fluid. Heat treatment destroyed the ability of uterine fluid to induce the acrosome reaction. Sperm incubated in the protein precipitate from uterine fluid showed all stages of the acrosome reaction. Thus, the uterine factor(s) responsible for inducing the acrosome reaction in buffalo sperm was associated with the non‐dialysable, albumin‐like protein fraction and was heat‐

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00907.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The effects of protein malnutrition during the nursing period on glycoprotein biosynthesis by testes of 20‐day‐old rats was studied. Pregnant Wistar rats were housed individually. On the day of delivery they were divided into two groups: one was fed a control diet (25% casein) and the other a low‐protein diet (8% casein) for a period of 20 days. Body and testis weights of pups suckled by the malnourished mothers were significantly lower than those of the pups suckled by normally‐fed mothers. The seminiferous tubules o malnourished rats showed a significant decrease in diameter and in the stage of development of spermatogenesis. Whole testes of normally‐fed 20‐day‐old rats showed significantly greater [2‐3H]mannose incorporation into glycoproteins than did the testes of malnourished rats of the same age. The microsomes of normally‐fed rats showed significantly higher GDP: mannose polyprenyl mannosyl transferase activity than did microsomes from malnourished rats, and this difference increased when exogenous dolichyl‐phosphate was added to the incubation medium. These results indicate that protein malnutrition decreases GDP: mannose polyprenyl mannosyl transferase activity in the microsomes of testes f

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00908.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Seminal zinc was measured in normospermic and infertile patients by a new colorimetric method. Analysis of semen from 5 patients with bilateral agenesis of the vas deferens showed high levels of zinc (mean value 1411.2 μg/ml). Lower levels were found in 6 patients with monolateral congenital or acquired obstruction (695.2 μg/ml). The seminal zinc level in 6 patients who had had a vasectomy or who had an epididymal blockage (125.7 μg/ml) was approximately the same as in 41 controls (134.6 ± 42 ± SD). Zinc levels were reduced in hypogonadal patients. These data suggest that zinc is secreted mainly by the prostate, while vesicular, epididymal and testicular secretions are devoid of zinc. Evaluation of this metal therefore permits the diagnosis of patency of the seminal pathways, but does not permit identification of prostatitis. No correlation was found between sperm count or motility and the seminal zinc level, nor between the latter and varico

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00909.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY