ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01300.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummarySixty‐one men referred to our laboratory for semen analysis, and subsequently judged to exhibit some form of sperm pathology, were asked to return for a second analysis, not less than 2 months after the first, in order to assess the stability of the pathological changes observed. In almost half of the cases, the referring physician had, on his own initiative, started hormone or antibiotic treatment. The sperm parameters studied included sperm count, sperm motility judged by laser‐Doppler spectroscopy, and sperm morphology and viability. The motility characteristics included percentage motile, their average velocity, and percentage swimming in a progressive manner, and their progressive velocity.In untreated subjects, there was no significant difference between the first and second analysis in any of the sperm parameters measured. This was also true for both oligozoospermic individuals (<20 × 106sperm/ml) and the group with higher sperm concentrations. All parameters were highly correlated on the two occasions. The average coefficients of variation of the paired observations were highest for sperm count (approximately 25%) and lowest for sperm velocities and the proportion of abnormal and viable cells in the ejaculate (1–9%). No major differences in the extent of variation could be detected between the low and high sperm density groups.In general, the unsystematic antibiotic and hormone regimens (clomiphene or androgen) used by the referring physicians had no discernable effect on any aspect of sperm quality, indicating the need for more controlled and standardized programmes of tre

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01301.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryThe immunohistochemical localization of lactoferrin in the normal human prostate, seminal vesicle, vas deferens, epididymis and testis was studied using the per‐oxidase‐antiperoxidase method at the light and electron microscopical level. Lactoferrin immunoreactivity was localized in the glandular epithelial cells and granulocytes in the prostate and seminal vesicle. In the prostate, lactoferrin showed an uneven distribution; some of the glands contained exclusively positive cells and others were completely lactoferrin netative, while the rest contained scattered positive cells. The seminal vesicles were divided into three segments, and their lactoferrin content varied significantly although it was always epithelial. The ductus deferens, epididymis and testis contained no lactoferrin. In conclusion, lactoferrin was found in the prostate and seminal vesicles, but not in the tes

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01302.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryA significant positive correlation was found between the liquefaction time of human seminal coagula and bound sialic acid. There was also a similar relationship between bound sialic acid and the enzyme sialyl‐transferase. This suggests that the degree of sialylation of the components of seminal coagulum are important in determining the liquefaction time of the coagulum. These results support previous findings.The coagulum is considered to be composed of glycoprotein‐metal ion complexes, and the initial stage of liquefaction results from the reduction of these metal ions by L‐ascorbic acid. The removal of hydrogen peroxide, generated by the oxidation of L‐ascorbic acid, requires the presence of glutathione peroxidase and glutathione reductase. These enzymes have been identified in human seminal plasma and their possible physiological importance is di

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01303.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryThe concentration of fructose in seminal plasma is an important variable that should be measured in the assessment of fertility status. The current method requires the boiling of diluted seminal plasma with resorcinol. The present study offers a new two‐point spot test which uses the metol method. The results of analysis of 104 seminal plasma samples are in excellent correlation with the resorcinol method. The reported method is easy to use, inexpensive, and can be introduced into any laborator

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01304.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryTo improve the quality of sperm for intra‐uterine insemination (IUI) or in‐vitro fertilization (IVF) swim‐up (SU) and migration‐sedimentation (MS) methods were compared, for the selection of morphologically normal and motile sperm. In 74 patients consulting for couple infertility, it was shown that MS gave a better yield of motile sperm than did SU. An improvement in morphology and motility was achieved, especially in asthenospermia (motility<40%) and/or teratospermia (normal shape<40%). The percentage of midpiece and tail abnormalities was lowered by the MS technique. It is proposed that the MS method be used for IUI and IVF, adapting the number of tubes to the initial sperm concen

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01305.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryImmunoglobulin G (IgG) spaces and permeability surface area products (PS) were calculated separately in the testis parenchyma, capsule and interstitial tissue in four age groups of rats to see if there were regional differences in microvascular permeability in the rat testis and if there were changes in PS with age. The results demonstrated a marked increase in PS to IgG at 27 days of age in the testicular interstitial tissue, whereas PS in the capsule did not show such consistent changes. Blood volumes per gram, as indicated by the 3 min IgG spaces, were considerably greater in the testicular capsule than in the interstitial tissue, although the weight of the capsule was only about one‐third of the interstitial tissue weight. Maximal IgG spaces were reached at 5 h in the testicular parenchyma, but in most groups only at 20 h in the capsule. IgG was demonstrated immunohistochemically in the testicular interstitial tissue from 33 days of age onward

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01306.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryProteins from secretions or homogenates of mice seminal vesicles were analysed by polyacrylamide gel electrophoresis. In homogenates about 15 bands were differentially induced with molecular weights (MW's) of 12, 13. 14, 15, 15.5, 71, 120 and 140 kD, or repressed molecular weights of 12.5, 14.3, 28, 30, 53, 73, 90–105 kD). The effects of castration were reversed by testosterone and dihydrotestosterone but not by oestradiol, progesterone or corticosterone. When the androgen‐dependence of proteins was investigated using radioactive methionine the protein spectra showed that about 12 bands with molecular weights of 13, 13.7, 14, 15, 15.5, 16, 20.5, 24, 37, 38.5, 56, 68, 96 and 180 kD were differentially induced or repressed by androgens. Of the induced proteins, those with low molecular weight (12–15.5 kD) were accumulated in significant amounts between 20 days and 30 days, coincident with the pubertal increase of androgens in the seminal vesicles. Those induced proteins with high molecular weight (71, 120 and 140 kD) appeared between 40 days and 60 days. The androgen‐repressed proteins were strongly evident in immature males, but disappeared after

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01307.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryTotal testicular cells derived from immature and pubertal rats were cultured under long‐term conditions. Somatic adherent cells proliferated in culture and produced collagen and proteoglycans. Collagen synthesis accounted for 25% and 5% of total protein synthesized by adherent cells derived from immature, and pubertal rats, respectively. Proteoglycan synthesis was higher in cells from immature than from pubertal rats. The proportion of different types of glycosaminoglycan chains (particularly hyaluronic acid and chondroitin sulphate) also varied according to the age of the donor. The results suggest that the synthesis of extracellular matrix components by somatic testicular cells is an age‐related process which probably plays an active role in spermatogene

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01308.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01309.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY