摘要:

SummaryThe influence of semen quality on fertilization rates in an in‐vitro fertilization (IVF) programme was studied by analysing both conventional semen parameters and computerized movement characteristics. The study was based on 407 inseminated oocytes which were obtained from 50 patients in 113 laparoscopies. Sperm concentration did not correlate strongly with the fertilization rate. Sperm motility and morphology were the most meaningful parameters in predicting fertilization success. A drop in fertilization rate was found when sperm motility or normal morphology were below 40%. Sperm velocity measured in semen was the only sperm movement parameter which correlated with the fertilization rate, albeit weakly. The latter was reduced when average sperm velocity in semen was<50 μm/sec. Conventional semen parameters seem to be more predictive of the fertilizing potential of an ejaculate than movement characteristics obtained by computerized image analys

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01321.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryEvaluation of male fertility is based predominantly on results from semen analysis and determination of the sperm concentration is one of the main parameters of the analysis. The availability of a fully automated videomicrographic digital image analyser would offer both an objective and rapid method for determination of the sperm concentration. In the present study the sperm concentration in 327 semen samples was determined by haemocytometer according to the World Health Organization guidelines, and also by a computer‐assisted digital image analyser system. Results were classified according to the routine procedure (haemocytometer) before statistical analyses. The computerized measurements caused a shift to the right in the frequency distribution of sperm concentration. Sperm concentrations were more often overestimated significantly (P5.0 × 106/ml. In sperm preparations with concentrations<5.0 × 106/ml (n= 75) there was still a significant (P<0.001) overestimation of the sperm concentration. These findings indicate that the CellSoft computer program has to be improved to allow correction for the particles present in seminal plasma which are not sperm. For determination of the sperm concentration in seminal plasma‐free preparations, an effective method to remove these particles is also necessary to obtain reliable results with the present computer‐assisted digital image an

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01322.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryAs part of an in‐vitro fertilization programme (IVF), semen levels of adenosine triphosphate, fructose, citrate, zinc, free L‐carnitine and glycerophosphocholine were measured in 178 men. None of these substances appeared to have any predictive value with regard to the fertilizing potential of the semen during

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01323.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryThe maturation of Sertoli cells at puberty is critical for the initiation and maintenance of spermatogenesis. Little is known about the factors which control Sertoli cell maturation. We have examined Sertoli cells in testicular specimens from 44 subjects, ranging in age from 6 months to 67 years, for reactivity with a mouse monoclonal antibody (M2A) using the immunoperoxidase reaction. We found positive reactivity with prepubertal but not postpubertal Sertoli cells, suggesting that the antigen defined by M2A was a marker of immature Sertoli cells. This marker may be useful for studying the factors which influence Sertoli cell maturation during development of the testis.

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01324.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryMaintenance and development of spermatocytes and round spermatids was studied in an in‐vitro incubation system. This system consisted of open tubule fragments from 26–day‐old rat testes, obtained after collagenase treatment. The tubule fragments contained Sertoli cells and spermatogenic cells up to and including a small number of early round spermatids. The number of primary spermatocytes and round spermatids in the tubule fragments was estimated using flow‐cytometric analysis, immediately after isolation and after 72 h of incubation. In addition, the activity of LDH‐C4in the tubule fragments was measured. After 72 h of incubation, the percentage of spermatocytes was reduced by 70–80%, but the percentage of spermatids was doubled. The total LDH‐C4activity per well was increased 2–3–fold during 72 h of incubation of the fragments. A modest improvement of the culture results was observed when a combination of FSH, insulin, retinol and testosterone was added to the medium. LDH‐C4activity was investigated to see whether it could be used as a quantitative marker of isolated and cultured spermatocytes and spermatids. It was observed that LDH‐C4activity per cell was decreased when spermatocytes and spermatids were isolated and/or incubated at 4°C. However, the cellular enzyme activity returned to control values during subsequent incubation of the cells at 32°C, either in the absence or presence of a protein synthesis inhibitor. Cellular LDH‐C4activity may be influenced not only by temperature, but possibly also by other cell isolation conditions. It is concluded that LDH‐C4activity may not be a reliable quantitative marker for the presence of spermatocytes and spermatids in culture, but should be used in combination with other analytical methods such as DNA estima

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01325.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryTo detect systematic bias in the results of routine semen analysis over time, monthly means of semen parameters determined by the recommended WHO methods were computed. The analysis was based on a total sample size of 1784 ejaculates and included 18 months of observation. In addition to slight changes of morphology estimates caused by a change of laboratory staff, a major bias in the measurement of sperm motility could be detected. This observation triggered a search for changes in protocols not previously given the required attention. It revealed that the newly introduced use of polypropylene syringes with a mounted needle for accurate measurement of seminal volume impaired sperm motility. More detailed investigation by computerized sperm motion analysis in 10 semen samples treated simultaneously in different ways revealed that predominantly it was the needle which caused the drop in proportion of motile sperm (glass cylinder: 50.3 ± 4.1% vs. syringe + needle: 26.6 ± 5.3%; mean f SEM) and not the contact with the plastic material alone (syringe alone: 43.4 ± 4.8%). Other motion parameters such as curvilinear velocity (36.0 ± 1.6 μm/sec), linearity (78.5 ± 8.4%) and lateral head displacement (3.8 ± 0.9) were not influenced by the different methods of handling. The results indicate that long‐term sampling of monthly means may serve as part of a quality control scheme in semen

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01326.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryLigated tubules from the corpus epididymidis of men and monkeys were incubated in medium containing horseradish peroxidase (HRP) as a marker for fluid‐phase endocytosis. HRP was localized by light and electron microscopy after 0, 15, 30 and 60 min of incubation. Movement between the cells was prevented by tight junctions, but bypass of this barrier was apparently achieved by an intracellular vesicular mechanism leading to a time‐dependent appearance of HRP in the lumen. Uptake of HRP into basal cells and capture by the lysosomal apparatus of principal cells were also observed. HRP‐filled vesicles also appeared in the basal, mid and apical cytoplasm of epithelial cells in the caput 1 h after injection of the tracer into the epididymal circulation of the monkey, suggesting that this pathway also operatesin

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1989.tb01327.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY