ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00870.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Male accessory gland infection (MAGI, epididymo‐prostato‐vesiculitis) with abnormal semen quality was rarely the only abnormality in infertile couples since it occurred in no more than 1.6% of 2871 couples evaluated in 7 centres during a 3‐year period. Both partners of 33 infertile couples with no other demonstrable abnormality than abnormal semen and MAGI consented to participate in a double blind trial and were treated with either doxycycline. 100 mg/day for 1 month (20 couples) or placebo (13 couples). Follow‐up during a total of 175 couple‐months included semen analysis and the recording of pregnancy. Pregnancy occurred in 2 of the doxycycline‐treated couples (10%) and in 1 of the placebo treated couples (8%), corresponding with conception lates per month of 1.9% and 1.5%, respectively. Sperm motility and, to a lesser extent, morphology showed improvement in both groups. Evidence of infection, namely increased numbers of white blood cells and positive sperm culture, disappeared in both the doxycycline‐treated and placebo group. It is concluded that features of MAGI in semen may regress spontaneously and are not influenced by the doxycycline treatment. The concomitant improvement of sperm motility and morphology still does not seem to enhance the probability

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00871.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The ultrastructure of a perfusion‐fixed gonad in a case of androgen insensitivity was studied using thin sections and freeze‐fracture replicas. The distribution and arrangement of intermediate filaments in Sertoli cells was visualized immunohistochemically using an antibody against vimentin. Leydig cells lacked Reinke crystals, but contained all of the cytoplasmic organelles involved in steroid synthesis and additionally several lysosomes. The basement membrane and the basal lamina of the testicular tubules were considerably thickened. The testicular tubules consisted of gonocytes and Sertoli cells which had an immature nuclear structure, incomplete development of intercellular junctions and a primitive distribution pattern of intermediate cytoplasmic filaments. The previously reported differences in electron density of Sertoli cell cytoplasm are a non‐specific feature without significance to Sertoh cell matur

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00872.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The motility of spermatozoa was higher at 6–22 h in Ham's F10 culture medium supplemented with 20–30% human serum than with lower proportions of serum or with 1% human serum albumin. Heat treatment (56°C, 1 h), charcoal extraction and dialysis (18 000 molecular weight cut‐off) of the serum did not reduce sperm motility suggesting that high molecular weight components are responsible for maintenance of motility. Renewing the medium (Ham's F10 with 30% serum) at 7 h resulted in better sperm motility and velocity at 22 h. At 22 h the pO2, pCO2, pH and sodium concentrations were not different in replenished and control cultures, but the concentration of glucose was higher and that of potassium lower if the medium was changed. These results suggest that addition of 20–30% human serum and renewal of medium at intervals is beneficial for sperm culture and may be of use in in vitro ferti

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00873.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Lysophosphatidylcholine, arachidonic acid and docosahexaenoic acid were found to have a concentration‐dependent inhibitory effect on the motility of human sperm, whilst phosphatidylcholine had no effect. Seminal plasma attenuated the sperm‐immobilizing potencies of these lipids. Because all of the three inhibitors of motility are hydrolytic products of phosphatidylcholine, and the catalytic enzyme, phospholipase A2, is known to be calcium dependent, it is suggested that calcium might inhibit sperm motility by activating phospholipase A2which in turn releases lysophosphatidylcholine and free fatty ac

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00874.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Male C57B1/6J‐ob/obmice (4 months old) and their homozygous lean controls were compared with respect to pituitary LH secretion and functional parameters of purified Leydig cells in vitro. Compared with controls, obese mice showed reductions in the following parameters: Plasma testosterone levels (reduced by 57%), hCG‐stimulated testosterone formation in vitro (by 31%), conversion of progesterone to androgens by Leydig cells (by 39%), and GnRH‐stimulated LH secretion (by 26%). Lipid accumulation and a 37% decrease in naphthylesterase activity in the Leydig cells as well as hyperplasia of pituitary gonadotrophs were observed histochemically in obese mice. The changes in testicular endocrine function in obese mice are interpreted as consequences of pituitary dysfun

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00875.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The status of spermatogenesis in Snell dwarf, little and congenitally hypothyroid mice was studied. In all of these mice with a hormone deficiency the seminiferous tubules were smaller in size and contained fewer spermatogonia, spermatocytes, spermatids and spermatozoa than did those of normal control mice. There was no substantial difference in the Johnsen score between the hormone‐deficient mice and normal control mice, but the former had underdeveloped seminiferous tubules with a corresponding paucity of germ cells, which may be partly responsible for the infertility of these mice. In the present study, growth hormone and thyroxine were administered separately to growth hormone‐deficient and thyroxine‐deficient mice, respectively. Such replacement therapy brought about an increase in cell counts of the seminiferous tubules and in sperm counts in both g

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00876.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Pigs of different ages were studied to assess the effect of age on the development of the testes after intra‐abdominal transection of the testicular artery and vein. Transection of the testicular artery and vein at different stages of sexual development had a variable effect on the growth and differentiation of the testes. Transection performed at a young age (2 months) had no effect on the development of the testes (previous study). The animals used in this study were between 3 and 12 months (adult) at the time of surgical intervention. Their testes appeared to be more sensitive to disturbance of the main blood supply. The alternative vascular pedicle (vasal artery) was unable to adapt sufficiently to the increased physiological requirements of the testis during development in more than 50% of the animals. The results obtained from adult males indicate that intra‐abdominal transection of the testicular vessels invariably has a deleterious effect on the tes

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00877.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The effect of electrostatic field treatment of the testis on the offspring of male rats was investigated. The results showed that treatment ranging from 1 to 7 kV caused reduced fertility, but no deaths occurred among the treated animals during the experiment. Observations at 3, 30, 60 and 90 days after exposure showed no recovery of fertility among the treated rats. Treatment with 6 or 7 kV caused congenital anomalies in the offspring, such as micropthalmy, elongation of the foreskin of the penis (praeputium‐like), ‘rounded face’ with omnidirectional hair growth, and narrow pelvis in adult female offspring. The anomalies might be caused by changes to the genetic material in the sperm. The sex ratio of offspring in the experimental groups was not significantly different from normal, suggesting that the number of male and female offspring was unaffected by treatment. The number of offspring with experimentally linked congenital anomalies decreased with

ISSN:0105-6263    

DOI:10.1111/j.1365-2605.1986.tb00878.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY