摘要:

AbstractWithCandida spec. H during phosphate starvation the changes occurring in the macromolecular cell composition and growth parameters were studies. In the absence of exogenous phosphate, but in the presence of carbon source (n‐alkanes or glucose) and nitrogen source, the cells exponentially grow some time, whereas cell composition undergoes several changes. Both the lated, especially withn‐alkanes as carbon source. The carbohydrate content increased only slight, although the carbon source was in excess. The results were discus

ISSN:0044-2208    

DOI:10.1002/jobm.19820220202

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractA novel method was proposed for screening of autoregulators by anthracycline‐producing strains ofStreptomyces griseus. By means of a modified cosynthesis procedure, an indicator strain was detected capable of producing both leukaemomycin and aerial mycelium only in the presence yielding organisms and their mutants, but also by a streptomycin‐producing strain ofStreptomyces griseusas well as its non‐differentiation deriv

ISSN:0044-2208    

DOI:10.1002/jobm.19820220203

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractFive non‐streptomycin‐producing non‐aerial‐mycelium‐forming mutants (Str‐Amy‐) ofStreptomyces griseusobtained either by spontaneous degeneration or during continuous cultivation of the high‐producing aerial‐mycelium‐forming parent strain HP (Str+Amy+) were checked with regard to the composition of mycelial lipid material. All the Str‐Amy‐derivatives differed from their ancestor strain HP by an increased ration of 12‐methyltetradecanoic acid (aC15:0) to isopalmitic acid (iC16:0) during growth on a chemically defined medium lacking branched‐chain amino acids. This finding attests alterations in the availability of precursors for the biosynthesis of methyl‐ branched fatty acids. The qualitative composition of phospholipids and other polar lipids in one mutant group was found to be similar to the progenitor strain but, additionally, both a yellow pigment and a neutral lipid component were produced in excess. A second type of mutant differed by its incapability to form ornithinolipids even under phosphate limitation. Changes of phospholipid composition were demonstrated in the course of fermentation. Formation of ornithinolipid was suppressed by an excess of inorganic phosphate in the medium, while the portions of phosphatidylethanolamine and cardiolipin increased strongly. Furthermore, the formation of ornithinolipids was influenced by nitrogen sources. These results suggest that the composition of membrane ofS. griseusvaries in dependence upon the composition of the mediu

ISSN:0044-2208    

DOI:10.1002/jobm.19820220204

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractAsporogenous streptomycetes, e. g.Streptomyces flavochromogenes and S. luteolutescens, become able to form fertile aerial mycelium when they are cultivated in close contact with fungi. This may be caused by the production and secretion of fungal metabolites which induce or stimulate the aerial mycelium formation. An attempt was made to characterize the stimulatory factor. Among the numerous fungi tested the most active ones wereCladosporium cladosporioidesandDrechslera erythrospila.

ISSN:0044-2208    

DOI:10.1002/jobm.19820220205

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractIn aerobic batch cultures in mineral medium with glucose of a respiration‐deficient mutant ofSaccharomyces cerevisiae, growth parameters were estimated and the heat evolved was measured by a flow microcalorimeter. A growth enthalpy of – 163.6 joule per mole of glucose consumed was measured. Under anaerobic conditions, the value was – 134.6 joule, closer to the expected for alcoholic fermentation alone. The difference was found to be due to cyanide‐resistant respiration under aerobic con

ISSN:0044-2208    

DOI:10.1002/jobm.19820220206

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractMannan of Candidautiliswas hydrolyzed in oligosaccharides of different chain lenghts. Mannohexaose and mannopentaose were shown to be the immunodominant group of this mannan. The oligosaccharides obtained were bound to sepharose by way of β‐(p‐aminophenyl) ethylamine. Immunoglobulins against mannohexaose down to mannodiose were obtained using affinity chromatography of the anti‐C.utilisserum. The affinity columns were filled with sepharose‐oligosaccharide gels. The serological activity was proved by agglutination and preci

ISSN:0044-2208    

DOI:10.1002/jobm.19820220207

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractIndirect immunofluorescence with antibodies to microtubular proteins has been used to investigate the microtubule system ofDictyostelium discoideumvegetative amoebae and the action of several compounds that interfere with that system. All the inhibitors tested show an antimicrotubular effect.Colchicine, vinblastine, nocodazole, thiabendazole, and isopropyl‐N‐phenyl‐carbamate (IPC) seem to act mainly by destroying microtubules leaving the appearance of nuclei associated organelles (NAOs) undisturbed. On the other hand griseofulvin also affects NAOs which disappear in treated

ISSN:0044-2208    

DOI:10.1002/jobm.19820220208

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractMutants with various levels of resistance to streptothricin were isolated fromEscherichia coliK12, strain A19 after mutagenesis with N‐methyl‐N'‐nitroso‐guanidine and ethylmethane‐sulfonate. Nourseothricin, a mixture of streptothricin F. and D was the selection agent. Spontaneous resistant mutants could not be found. The streptothricin‐resistant mutantE. coliA19 Stcr 2/2/1 shows cross‐resistance to some of the aminoglycoside antibiotics investigated, but no cross‐resistance to chloramphenicol and chlortetracyclin. These results indicate similar mechanisms of action of streptothricin and aminoglycos

ISSN:0044-2208    

DOI:10.1002/jobm.19820220209

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractIn the River Saar, the distribution of methane‐oxidizing bacteria and their metabolic activity were determined in vertical and longitudinal profiles. At the sediment surface about two orders of magnitude more methane oxidizers were detected than in the overlying water. In the river as well as in laboratory experiments, the rate of methane oxidation was closely related to the concentration of methane. Most of the methane produced by methanogenic bacteria in the sediment escaped into the atmosphere. On the average only 1.2% of the produced methane was oxidized in the water phas

ISSN:0044-2208    

DOI:10.1002/jobm.19820220210

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19820220212

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY