摘要:

AbstractSeveral yeast strains can grow with good yield (0.16 to 0.19 mg protein/mg carbohydrate) on nitrogen supplemented Jerusalem artichoke extract. The most promising strain isLipomyces starkeyi.Including by‐products (pulps, proteins of extract), protein production can reach 2 metric tons/h

ISSN:0044-2208    

DOI:10.1002/jobm.19830230402

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

摘要:

AbstractThe gene‐enzyme relationships of thearommultienzyme complex ofSchizosaccharomyces pombethat catalyzes steps two through six in the prechorismate polyaromatic amino acid biosynthetic pathway have been studied. The various mutants were subjected to biochemical analysis by direct enzymic assays. These studies have established thataro‐3A,aro‐3B,aro‐3C,aro‐3D, andaro‐3E mutants lack, respectively, the enzymic activities 5‐dehydroquinate synthase, 5‐dehydroquinase, shekimate kinase, 3‐enolpyruvylshikimate 5‐phosphate synthase, and shikimate: NADP oxidoreductase. InS. pombelack enzymic activities for the inducible quinate catabolic pathway. The functional significance of thearomagg

ISSN:0044-2208    

DOI:10.1002/jobm.19830230403

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

摘要:

AbstractA synthetic medium containing ammonium nitrate, urea, D‐glucose, glycerol, and sodium glutamate was developed for growth ofXanthomonas oryzaeand preparation of its phytotoxin. Culture filtrates and partially purified toxin preparations caused blight symptoms when inoculated in leaves of 30 days old rice plant cv. T(N) I. Toxin elicited quicker response than living cells. This activity was due to a non‐dialysable, partial acid and heat labile glycopeptide. There was a sharp decrease in activity when culture filtrates and crude toxin preparations were heated at 100°C for 30 min. Maximum activity was observed in pH range of 6.0–7.0 and a gradual loss at lower pH (5.0–3.0) and higher pH values (8.0–10.0). Unlike living cells which caused continuous lesion enlargement, toxin inoculation caused maximum attainment of lesion length within 4–6 weeks. Toxin preparation in concentration of 45 μg glucose/ml failed to evoke response.The study demonstrates that the purified toxin being easy in storage and causing quicker response than living cells when inoculated in rice leaves, may be used to screen rice varieties for bacterial blig

ISSN:0044-2208    

DOI:10.1002/jobm.19830230404

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

摘要:

AbstractTwo newly isolated aniline‐degrading bacterial strains were characterized with regard to their enzyme systems responsible for aniline catabolism. One of them identified as aRhodococcussp. metabolized aniline exclusively via the β‐ketoadipate pathway by means of inducible enzymes. The aniline‐degrading enzyme system of the second isolate, presumably a pseudomonad, was shown to consist of an inducible aniline‐converting enzyme and constitutive meta‐pathway enzymes. Both isolates failed to metabolize monochlorinated anilines in the absence of additional carbon sources. To explain this the ring‐cleaving enzymes of both isolates were examined for their substrate specificities. Furthermore, the effect of 4‐chlorocatechol on the enzymes catalyzing aniline conversion and catechol oxygenation wa

ISSN:0044-2208    

DOI:10.1002/jobm.19830230405

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

摘要:

AbstractA rapid and convenient method for producing protoplasts from 3 d old mycelium of the ascomyceteHypomyces ochraceusis described. The procedure involves aHelix pomatiaenzyme preparation and sucrose (20%) for stabilization. Pretreatment with disulfide bond reducing agents reduced the amount of viable protoplasts. Formation of protoplasts and different stages of regeneration were observed by phase contrast microscopy. There was only one type of true regeneration from protoplasts to hyphae in 15–30% gelatine medium by direct forming a germ tube from the original protoplast. Cytological events and physiological conditions are discusse

ISSN:0044-2208    

DOI:10.1002/jobm.19830230406

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

摘要:

AbstractAddition of oxygen‐containing C1‐compounds to chemostat cultures of GB 25 increases both the yield of biomass and the specific growth rate. At optimum concentrations the catalytic activity of these compounds increases with increasing growth rates. Their influence on maintenance coefficients and maximum yield coefficients decreases in the order CH3OH>CO2>HCOOH>HCHO. This result together with spectrophotometric NADH determinations suggests that the NADH pool determines the balance between the assimilatory and oxidative utilization of formaldeh

ISSN:0044-2208    

DOI:10.1002/jobm.19830230407

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

摘要:

AbstractThe significance of the sequential processes of phosphate limitation and of phosphate release from medium constituents is demonstrated in technical streptomycin fermentations. The phosphate limitation initiated the streptomycin synthesis as well as the formation of phosphatases and protease. In later periods of the process the phosphate release influences especially the enzyme formation.

ISSN:0044-2208    

DOI:10.1002/jobm.19830230408

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19830230411

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19830230413

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19830230416

出版商:Akademie‐Verlag    

年代:1983

数据来源: WILEY