摘要:

AbstractAus 5 Großgebieten Brasiliens wurden 36 Bodenproben entnommen und nach vier verschiedenen Isolierungsmethoden auf Fruchtkörper‐bildende Myxobakterien untersucht. Je Bodenprobe wurden 100 Einzelproben angesetzt, insgesamt 3636.Es wurden 19 verschiedene Fruchtkörper‐bildende Myxobakterien‐Arten isoliert und nachBERGEY(MCCURDY1974) bestimmt. Sie gehören folgenden 7 Gattungen an:Myxococcusmit 5 Arten,Archangiummit 3 Arten,Cystobactermit 2 Arten,Polyangiummit 4 Arten,Melittangiummit 1 Art,Stigmatellamit 2 Arten.Chondromycesmit 2 Arten.Zu den häufigsten und weitverbreiteten Myxobakterien gehören die ArtenM. fulvus, M. coralloidesundA. gephyra, zu den selten beobachteten die ArtenA. flavum, Stigm. erecta, Stigm. aurantiaca, Ch. apiculatusundCh. pediculaius.Artenzahl und Häufigkeit der gefundenen Myxobakterien werden in Beziehung zur Isolierungsmethode und zum Herkunftsgebiet der Bodenpr

ISSN:0044-2208    

DOI:10.1002/jobm.19780180202

出版商:Akademie‐Verlag    

年代:1978

数据来源: WILEY

摘要:

AbstractCell extracts ofCryptococcus laurentii, a yeast which synthesizes amylose when grown at low pH, contained an α‐glucan phosphorylase (α‐1·4)‐glucan: orthophosphate glucosyl transferase (EC 2.4.1.1). The enzyme, which is very labile, showed greatest stability at pH 8.0 in the presence of 0.1 m β‐glycerophosphate and 0.1 m sodium fluoride. Based upon visible colour development with iodine a cell homogenate, centrifuged at 32,000 g to remove cell debris, synthesized starch‐like polymers upon incubation with glucose‐1‐phosphate in a pH range of 5.6‐6.1. Based upon incorporation of labelled glucose from glucose‐1‐phosphate the pH of maximum activity was 5.9‐6.2. The enzyme was partially purified by glycogen complexing, precipitation, and adsorption on hydroxylapatite followed by elution. The purified enzyme has a Kmfor glucose‐1‐phosphate of 1.6 × 10−2m. Mutants impaired in their ability to synthesize amylose contained approximately one fourth of the phosphorylase activity as compared to the wild type. The possible role of the glucan phosphorylase in a

ISSN:0044-2208    

DOI:10.1002/jobm.19780180203

出版商:Akademie‐Verlag    

年代:1978

数据来源: WILEY

摘要:

AbstractStreptomyces spec.5s accumulates in the culture fluid lytic activities against living cells ofCandida guilliermondiiH. From the culture fluid a freeze‐dried dialyzed ammonium sulfate precipitate was prepared. Except β‐glucosidase‐activity it showed activity of α‐mannosidase which was characterized. Separation of both enzymes by chromatography on polyacrylamide gel was

ISSN:0044-2208    

DOI:10.1002/jobm.19780180204

出版商:Akademie‐Verlag    

年代:1978

数据来源: WILEY

摘要:

AbstractThe wild typeNostoc muscorum(UW strain) has yielded various physiological mutants altered in utilization of sulphate, following mutagenic treatments with N‐methyl, N′‐nitro N‐nitrosoguanidine (NTG). One of the mutant strains designated as Sat‐20 failed to grow in a medium containing sulphate (MgSO4· 7 H2O). However, the mutant strain could grow when supplemented with thiosulphate (Na2S2O3· 5 H2O), while methionine could fulfil the sulphur requirement only partially. On comparative reasons, the wild type as well as the mutant showed preference for thiosulphate over other sulphur sourc

ISSN:0044-2208    

DOI:10.1002/jobm.19780180205

出版商:Akademie‐Verlag    

年代:1978

数据来源: WILEY

摘要:

AbstractIn the agar diffusion test 24 triazines were investigated with regard to their action on the multiplication of DNA phages (λ and LPP‐1) and RNA phages (M 12 and Qβ). In several cases the amount of plaques was diminished or increased depending on the kind of triazine and virus. The investigations demonstrate the triazines to be able to interfere with the formation of plaques by virulent and temperate viruses of procaryo

ISSN:0044-2208    

DOI:10.1002/jobm.19780180206

出版商:Akademie‐Verlag    

年代:1978

数据来源: WILEY

摘要:

AbstractThe fine structure of the cortex of the natural sclerotium ofClaviceps purpureawas studied. The cell wall of sclerotial cells is thickened due to overproduction of the fibrillar component of the wall. The intercellular spaces of the cortex tissue form a continuous system which is apparently instrumental in mediating communication between the growing sclerotium and the external milieu. The cytoplasmic membrane of imbibed sclerotial cells carriers abundant signs of secretory activity. Secretion vesicles, the content of which is discharged into external space, apparently contain exo 1,3‐glucanase. Cytoplasmic vesicles migrating toward the plugged pores of the thickened septa apparently involve hydrolase secretion too. Spherosomes with lipid content are a predominant component of the cytoplasm of sclerotial cells. The activity of the membrane systems of imbibed cells indicates that the mobilization of lipids sets in only after activation of the hydrolases. Findings of phagocytosis of lipid granules by vacuoles are relatively frequent so that lipolysis might proceed in the vacuoles. Alkaloids could not be detected with the aid of freeze‐etch

ISSN:0044-2208    

DOI:10.1002/jobm.19780180207

出版商:Akademie‐Verlag    

年代:1978

数据来源: WILEY

摘要:

AbstractThe maximum cell yield (Y cM‐ g biomass carbon per gram substrate carbon) and the rate of maintenance metabolism (mc‐ g substrate carbon/g biomass carbon per hour) have been determined for substrate limited continuous cultures ofPseudomonas fluorescensandP. aeruginosa.The metabolism of the organic substrates was monitored by measuring the COD‐removal1) rates at different dilution rates. The advantages of expressing yield and maintenance coefficients in carbon units is di

ISSN:0044-2208    

DOI:10.1002/jobm.19780180208

出版商:Akademie‐Verlag    

年代:1978

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19780180209

出版商:Akademie‐Verlag    

年代:1978

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19780180210

出版商:Akademie‐Verlag    

年代:1978

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19780180213

出版商:Akademie‐Verlag    

年代:1978

数据来源: WILEY