摘要:

AbstractL‐Tryptophan did not exert any influence on peptide alkaloid formation in an ergotamine and in an ergosine‐accumulatingC. purpureastrain. A different picture was observed in a series of relatedC. purpureastrains. Tryptophan showed a slight stimulatory effect on the ergotoxine producer Pepty 695/S. A blocked mutant of it, designated as Pepty 695/ch which was able to accumulate secoclavines gave similar results. In a high‐yielding elymoclavine strain Pepty 695/e, the progeny of the former one, tryptophan up to a concentration of 25 mm stimulated remarkably clavine biosynthesis. Furthermore, tryptophan could overcome the block of synthesis by inorganic phosphate. Increased specific activities of chanoclavine cyclase but not DMAT synthetase were observed in cultures of strain Pepty 695/e supplemented with tryptophan. 5‐Methyltryptophan and bioisosteres of tryptophan were ineffective in alkaloid stimulation. These results are compared with those obtained with the grass ergot strain SD 58 and discussed with the relation to other induction ph

ISSN:0044-2208    

DOI:10.1002/jobm.19840241002

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

摘要:

AbstractGrowing cells of blue‐green alga,Anacystis nidulans, were treated with lysozyme in osmotically stabilized nutrient medium. Their development and regeneration ability was studied in liquid media. During 5–8 hrs about 95% of cells were converted to osmotically sensitive spheroplasts having a residual wall layer on their surface. The highest viability of spheroplast population was achieved using egg‐white lysozyme for spheroplast preparation and gradual dilution by nutrient medium instead of centrifugation for lysozyme removal. Most of such prepared spheroplasts survived for 3 days of cultivation in nutrient medium, and started to die gradually from 3 to 10 days. Spheroplasts were growing spherically, about 30% of them were growing for 8 days up to 40‐fold increase in volume. Multiplication of thylakoid cisternae in growing spheroplasts was proved by freezeetching. Electron microscopy further revealed newly synthesized amorphous walls of isodiametrical appearance by growing spheroplasts resembling the original cell walls of cells. In liquid media blue‐green alga spheroplasts ofAnacystis nidulanscan grow for a number of days, however, only regeneration of incomplete wall occur unable to promote reversion of spheroplasts

ISSN:0044-2208    

DOI:10.1002/jobm.19840241003

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19840241005

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

摘要:

AbstractAcinetobacter calcoaceticus69‐V is not able to grow on carbohydrates or its derivatives and to use them as sole carbon sources. On the other hand, the partial oxidation of several monosaccharides (glucose, galactose, mannose, xylose, arabinose, ribose) by intact cells has been demonstrated by acid production and measurements of oxygen consumption.Glucose and fructose are taken up by intact cells. Different transport systems exist for the uptake of both hexoses. The “apparent”KM‐values for the membrane transport amount to 1.5 · 10−3and 1.7 · 10−4moles/l for glucose and fructose, respectively. Gluconolacton only inhibits the transport of glucose, whereas the transport of both hexoses is inhibited by uranylacetate. Other monosaccharides and metabolic inhibitors are without effect. An exchange of intracellular against extracellular glucose has not been found.Gluconate and CO2have been identified as extracellular products of glucose oxidation after an incubation period of more than 5 min, whereas gluconate and 6‐phosphogluconate have been detected as intracellular products. No oxidation products have been found using fructose as substrate.No activity of hexokinase, phosphofructokinase, pyruvate kinase, and 6‐phosphogluconate dehydrogenase have been detected in cell‐free extracts of sonicated cells. An aldose dehydrogenase coupled with an electron transport system occurs in the membrane fraction. The presence of this enzyme was demonstrated by the reduction of dichlorophenolindophenol as well as by oxygen consumption which is inhibited by cyanide.The aldose dehydrogenase exhibits a broad substrate specificity against different aldohexoses and aldopentoses differing by theirKM‐values. Cytochromeband the terminal oxidase cytochromeoare components of the electron transport system. Neither hydrogen peroxide is formed, nor pyridine nucleotides participate in a

ISSN:0044-2208    

DOI:10.1002/jobm.19840241006

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19840241008

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

摘要:

AbstractProtein patterns of conidia produced by aStreptomyces griseusstrain in submerged cultures and on solid media were compared. Cell‐free extracts (30000 X g supernatant) were prepared and analyzed on gradient SDS polyacrylamide gels. The protein patterns of both kinds of conidia were found to be practically identical, and they differed from protein patterns of the old vegetative hyphae in a characteristic wa

ISSN:0044-2208    

DOI:10.1002/jobm.19840241009

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

摘要:

AbstractIn strongly acidic medium (70% HClO4) streptothricins form a fluorophore (λex= 312 nm; λem= 381 nm) with unknown structure. A fluorimetric determination of pure or crude products and cultures, respectively, was worked out based on this reaction. Concentrations for fluorescence measurements were in the range of 10−8— 2 · 10−7moles. Interferences of the assay are discussed, a statistical evaluation of results and a comparison between microbiological and fluorimetric findings a

ISSN:0044-2208    

DOI:10.1002/jobm.19840241010

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19840241011

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

摘要:

AbstractThe phosphorylation of dihydroxyacetone by means of the enzyme dihydroxyacetone kinase (DHAK) is a key reaction in the assimilation of methanol in yeasts. In the present paper the influence of varying concentrations of Mg2+ions and ATP upon the reaction rate of DHAK fromCandida methylicais described and the regulatory consequences for the assimilation of methanol by yeasts are discussed.

ISSN:0044-2208    

DOI:10.1002/jobm.19840241012

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19840241013

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY