摘要:

AbstractTwo strains of Tobacco Mosaic Virus (TMV) isolated from tomato plants were characterized by biological, biochemical, immunological and electron microscopical techniques including immuno‐electron microscopy. The biological tests indicated, on the basis of symptoms observed on differential hosts, differences between the two strains. However, detailed studies with the other techniques mentioned above did not reveal significant difference

ISSN:0044-2208    

DOI:10.1002/jobm.19840240802

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

摘要:

AbstractTwo series of aerial‐mycelium‐negative (Amy−), anthracycline‐nonproducing (Ant−) mutants were obtained from ancestral Amy+Ant+strains ofS. griseus: a) derivatives represented by themet−strain 39 which could not differentiate although they were still producing both the butyrolactone‐type autoregulator1and NADP‐glycohydrolase, and b) mutants whose incapability to form spores and anthracycline pigments was apparently caused by the loss of autoregulator production. These latter mutants responded to the addition of1or the naturally occurring dihydro derivative2with complete or at least partial reconstitution of differentiation‐associated functions. All of the b)‐type mutant strains exhibited similar biochemical alterations in the presence of1or2regardless of the presence of additional genetic changes in the primary metabolism. Two mutants, however, displayed an altered pattern of secondary product formation. In submerged cultures the major biochemical changes observed in presence of1(or2) were an increase of the lipid level in the mycelium, an alteration of the lipid composition, and a stimulation of neutral proteinase production. All of the blocked autoregulator‐negative mutants were discernible from the ancestral strains and strain 39 by their lack of NADP‐glycohydrolase production. This suggested the existance of a common genetic locus or a common pleiotropic regulator gene controling both gene functions. Present ideas concerning the role of butyrolactone‐type autoregulator1as a pleiotropic effector molecule interacting with development ofS. griseusare summarized

ISSN:0044-2208    

DOI:10.1002/jobm.19840240803

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19840240804

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

摘要:

AbstractVarious blocked mutants were isolated from three leukaemomycin‐(daunomycin‐)producing strains IMET JA 3933, IMET JA 5142 and IMET JA 5570 ofStreptomyces griseusby NTG and UV treatments. Among them, one class of four mutants ZIMET 43707/1P, IMET JA 5570/3P, IMET JA 5570/10P and IMET JA 5142/01P1 produced new blue and red pigments. Two red compounds designated 1PI and 1PII are the main components of the pigment complex produced by culture of the blocked mutant ZIMET 43707/1P. This paper describes the isolation of 1PI and IPII; furthermore, the spectral and physicochemical properties of these anthracyclinones and the elucidation of their structures are repor

ISSN:0044-2208    

DOI:10.1002/jobm.19840240805

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

摘要:

AbstractHybrids produced by protoplast fusion between auxotrophic mutants of the same strain contained the parental markers heterozygously, as indicated by induced mitotic segregation. Even within the same fusion combination the hybrids differed in their mitotic stability and their induced mitotic segregation pattern. The occurrence of aneuploids was proved. The ploidy of the hybrids did not exceed the diploid level in any case.

ISSN:0044-2208    

DOI:10.1002/jobm.19840240806

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19840240808

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

摘要:

AbstractThe efficiency of protoplast fusions in haploid strains ofP. guilliermondiiwas dependent on the strain used. In the primary fusion colonies, a large proportion of non‐dividing cells was sometimes observed. While in two strains stable hybrids occurred immediately after fusion, one strain showed a longer‐lasting heterokaryotic st

ISSN:0044-2208    

DOI:10.1002/jobm.19840240809

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

摘要:

AbstractFor development of breeding stocks ofS. lipolyticafor genetic investigations we isolated and identified several auxotrophic mutants. After selection of strains with normal meiotic segregation pattern for the markersmetA, lysA, argA, leuA, uraA, hisAit was shown that the locimetAandlysAand the lociargAandleuAare linked, crossing over frequencies are 25 ± 0.9% and 30 ± 1.2%, respectively.We identified several complementation groups. The markeruraAis allel toura3from strains of the Donner Laboratory (USA).These results are the basis for selection of special mutants for forthcoming investigations.The existence of linkage fragments makes it possible to use these strains as tester strains for genetic analysi

ISSN:0044-2208    

DOI:10.1002/jobm.19840240810

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

摘要:

AbstractProtoplast fusion of genetically markedSaccharomycopsis lipolyticastrains was carried out succesfully. Mitotic segregation was induced to prove the presence of parental markers in fusion products. Methylbenzimidazol‐2‐yl‐carbamate has been shown to induce efficiently mitotic segregation in this yeast. The segregation pattern of genetic markers in a sexual hybrid was compared to that of a parasexual h

ISSN:0044-2208    

DOI:10.1002/jobm.19840240811

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY

摘要:

AbstractThe results present evidence for the important role of phosphate‐mediated regulation of the nourseothricin biosynthesis by the processes of phosphate limitation and release of phosphate. Also, higher initial concentrations of phosphate were found to have a strong inhibitory effect on the biosynthesis of nourseothricin. It is concluded that the initial phosphate concentration was the primary target for the biometrical optimization of the fermentation medium. The presence of zinc ions neutralized the negative effect of high initial concentrations of phosphate which was also strongly influenced by the regime of sterilizatio

ISSN:0044-2208    

DOI:10.1002/jobm.19840240812

出版商:Akademie‐Verlag    

年代:1984

数据来源: WILEY