摘要:

AbstractThe effect of PCNB in various concentrations on the ultrastructure ofMucor mucedoandPhytophthora cactorumwas analyzed after an incubation period of 2 hours. The most striking effect in both fungi was a diffuse lysis of the internal structure of the mitochondria which differs markedly from the lysis induced by etridiazol (terrazol). Moreover an enlargement of the perinuclear space and an increased formation of vacuoles was observed.InMucor mucedo, but not inPhytophthora cactoruma pathological thickening of the cell wall was observed. Although after 2 hours incubation with PCNBPhytophthoragave similar ultrastructural reactions in the mitochondria asMucor, in growth experiments on agar dishes this species was 5–10 times less sensitive to PCNB compared toMuco

ISSN:0044-2208    

DOI:10.1002/jobm.19820220402

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractThe ultrastructure ofn‐alkane‐grown cells ofCandida spec.H was investigated by thin sectioning and freeze‐etching techniques. On the plasma membrane shallow depressions could be observed.n‐Alkane deposits were localized within the cell wall and at the plasma membrane. Periplasmic multilamellar structures appear in connection withn‐alkane accumulation. Their participation in hydrocarbon assimilation and the nature of the lamellar structures are

ISSN:0044-2208    

DOI:10.1002/jobm.19820220403

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractA Gram‐ negative rod‐shaped bacterium 28/1 isolated by enrichment cultures is able to hydrolyze the amide bond of some phenylurea herbicides and acid anilide herbicides by an inducible amidase. 7.5% of 0.3 μmol. ml−1linuron (3‐(3,4‐dichlorophenyl)‐1‐methoxy‐1‐methylurea) are hydrolyzed after 16 hours. 1,1‐Dimethylphenylureas are not degraded. Acid anilides are hydrolyzed at a higher rate, 80% of 0.5 μmol. ml−1N‐(4‐chlorophenyl)‐propionamide and N‐(4‐nitrophenyl)‐propionamide are transformed after 6 hours. The 1‐methoxy‐1‐methyl phenylureas are effective inducers. Linuron‐induced cells have a specific activity of 3—4 nmol per mg dry weight per min on the substrate N‐(3,4‐dichlorophenyl)‐propionamide (Propanil). The rate of hydrolysis is influenced by substituents of the aniline ring and by t

ISSN:0044-2208    

DOI:10.1002/jobm.19820220404

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractAn anomalous cell wall thickening inMucor mucedois induced already within 60–120 min by some fungicides (etridiazol, chloroneb, pentachloronitrobenzene, dicloran, drazoxolon, biphenyl) as well as with a N2‐atmosphere or high concentrations of glucose, but not with 2,4‐dinitrophenol, chlorinated phenols, dichlofluanid and antimycin A. This effect seems to be identical to the change from the mycelial (M‐) to the yeast (Y‐) form in dimorphic fungi, which can be achieved by culture conditions as well as by addition of chemicals. The cause seems to be a specific, complex change in the metabolic state. A scheme of regulation is presented which explains most of the experimental results described

ISSN:0044-2208    

DOI:10.1002/jobm.19820220405

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractWe localized the gene for resistance to phage SPO1 relatively to the markerspur B 34anduraby means of the polyethylene‐glycol induced fusion of bacterial protoplasts of three‐fold auxotrophicBacillus subtilisstrains S3 and S13. By this same method, the site of some auxotrophic markers was tentatively determined. The application of the protoplast fusion technique to exact genetic analysis will not be possible until the exo‐ and endogenous factors influencing cell wall regeneration are standardized. Fluctuations of this kind are very significant for the determination of genetic segreg

ISSN:0044-2208    

DOI:10.1002/jobm.19820220406

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractDue to the increasing world population, supply of steroids is dwindling. New sources of steroids is dwindling. New Sources of steroids may be found and the existing steroids and sterols may be better utilized. The role of biotechnology in the production and efficient utilization of steroids and sterols is discussed.

ISSN:0044-2208    

DOI:10.1002/jobm.19820220407

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractThe important effect of release of soluble phosphate from complex media constituents during the turimycin fermentation is demonstrated by investigation of process kinetic and phosphate balance. The observed changes in physiological parameters and the diauxic growth are discussed in connection with extracellular enzymes formation hydrolyzing phosphate‐containing complex media constituent

ISSN:0044-2208    

DOI:10.1002/jobm.19820220408

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

摘要:

AbstractThe maintenance of the plasmidpBR 322inE. coliK12 C600recB recCwas investigated during successive discontinuous cultures. The percentage of tetracyclin resistant cells, the plasmid content and the β‐lactamase activity decrease during growth, indicating the segregation of plasmid‐free cells. The amount of plasmid‐free segregants which overgrow the plasmid‐containing bacteria, depends on the generation number of growth. By contrast, in C600rec+(pBR 322) the plasmid is maintained more stable during prolonged incubation. These results suggest the involvement of therecB recC‐gene product in plasmid m

ISSN:0044-2208    

DOI:10.1002/jobm.19820220409

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19820220412

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY

ISSN:0044-2208    

DOI:10.1002/jobm.19820220413

出版商:Akademie‐Verlag    

年代:1982

数据来源: WILEY