摘要:

Forty‐eight isolates obtained from pasteurized milk and cream, raw milk, and various sites on a dairy farm were identified asYersinia enterocoliticabiotype 1 but were unusual in their ability to ferment lactose. The majority belonged to serotype 5a. Serotypes 5b, 6, 13/15 and 23/15 were also found. A few isolates were not typable. There is no evidence that strains isolated in this study are pathogenic for humans. Their presence in pasteurized products and growth at refrigeration temperatures are, however, cause for concer

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1979.tb02589.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The numbers of salmonellas in raw sewage entering a treatment plant varied hourly and diurnally; their peak concentration preceded the peak influent waste water flow into the plant by about 2 hours. Salmonellas were detected in all raw influent samples collected from 2 sewage works and the mean population level at the daily peak period was 3000 organisms/1. On average. 70–80% of the salmonellas were removed during primary sedimentation when upwards of 74–84% of solids were removed. Biological treatment and secondary sedimentation removed a further 70–100% of the pathogen. Compared with the activated sludge process the trickling filters were less efficient in removing salmonellas and they were adversely affected by increased loading following rainfall. Considering the whole treatment process, the Guildford works with its activated sludge treatment removed an average of 99–83% salmonellas while the Woking works with its trickling filter plant removed an average of 93–04% of the organisms. The large variance in the numbers of salmonellas in the final effluent from the trickling filters suggest that greater emphasis should be placed on the actual quality of the effluent rather than on percentage removal eff

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1979.tb02590.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

1.Salmonella heidelberg, chilled from 37 to 5°C in glucose‐salts broth, grew better on a simple medium (glucose‐salts agar) than on a complex medium (Tryptic Soy Agar + 0·5% yeast extract).2. The organisms recovered the ability to grow on the complex medium after a further 8 h incubation at 5°C.3. RNA synthesis would appear to be a critical factor in the recovery p

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1979.tb02591.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

A fluorescent antibody technique was developed for the determination of the capsular‐type of strains ofStaphylococcus aureus.It compared favourably with the method using serum‐soft agar (Yoshida 1972). With the new technique, many populations of encapsulated and unencapsulated strains were investigated. Of 1421 fresh isolates ofStaph. aureus, 54 were encapsulated and among these 54·8% and 48·1% were mono‐ and polyvalent, respectively. Capsular‐type antigens A and B were found in 92·5% and 44·4% of strains respectively; capsular‐types C and D were found relatively infrequently. In the other group, of unencapsulated strains, capsular‐type antigen production was demonstrated in 125 out of 163 strains examined. Mono‐ and polyvalent capsular‐types (A and B antigen producing strains) comprised 77·6% and 22·4%. respectively. In these capsular‐types A and B were found in 54·4% and 62·4%, respectively: capsular‐type antigen C and D producing strains were again infrequent. These results indicate that a majority of ordinaryStaph.aureusstrains produce capsular‐type antigens although isolation of the encap

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1979.tb02592.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

A semi‐automatic method for the detection of salmonellas in food products is described. This method involves measurement of the fluorescence intensity of preparations stained with fluorescent antibody. An overall agreement of 89·6% was obtained when the method was compared with a standard cultural technique for the detection of salmonellas in foods, feeds and environmental samples, although false negatives were a problem with raw meat products. A saving in time of at least 18 h was achiev

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1979.tb02593.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Staphylococcus aureusandEscherichia coliincubated at 1° or 4°C became increasingly sensitive to Mannitol Salt Agar and Violet Red Bile Agar respectively. The increased sensitivity was most marked with exponential phase cultures.Streptococcus faecaliswas refractory to similar treatments. The viability of all 3 organisms on selective and non‐selective media is repor

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1979.tb02594.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Total viable counts, proteolytic, ureolytic, cellulolytic and NH+4utilizing anaerobic bacteria in caecal contents, soft and hard faeces of rabbits were studied. No significant differences between the bacterial counts from the caecum and soft faeces were observed (P<0·05) but there were significant differences between the bacterial counts of the soft and hard faeces (P<0·01

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1979.tb02595.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Staphylococcus aureusincubated in Tryptic Soy Broth at 1°, 3°, 5° and 7°C became increasingly sensitive to Mannitol Salt Agar. Injury, as measured by salt sensitivity, decreased with increasing temperature from 1° to

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1979.tb02596.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

1. ThirteenErwinia carotovorastrains produced mannose‐sensitive haemagglutinins associated with the presence of type‐1 fimbriae.2. Three fimbriateEr. rhaponticistrains produced haemagglutinins of the mannose‐resistant and eluting (MRE) type.3. There were 12Er. carotovora, 12Er. atrosepticaand 2Er. chrysanthemistrains that produced no detectable haemagglutinins and were non‐fi

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1979.tb02597.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

1. A more reliable, time‐saving and easily performed technical modification of the method for direct and indirect immunofluorescence of mycoplasma colonies, involving the use of steel rings to hold the agar‐block specimens, is descri

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1979.tb02598.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY