摘要:

SUMMARY:Deoxyribonucleic acid (final concentration 100 μg/ml) extracted from wild‐type cells ofMethylococcus capsulatuswas used to transform ap‐amino benzoic acid (PABA) requiring strain (paba‐). Maximum levels of transformants were obtained towards the end of the exponential phase of growth in shake flask culture and the level of the population of transformants was not significantly altered by post‐treatment incubation in enriched medium. The maximum transformation frequency was 0.17% of the initial population of PABA requiring cells and the back mutation frequency was 1 : 3.

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1971.tb01005.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

SUMMARY:A strain ofAcinetobacter Iwoffii, isolated from a stored sample of distilled water, hydrolysed acetylsalicylic acid to salicylic and acetic acids. It grew in mineral salts medium with either of these compounds as C source and NH4+as N source. Experiments with whole cells and cell free extracts and the isolation of intermediates showed that acetylsalicylic acid was metabolized through salicylic acid, catechol,cis‐cis‐muconic acid, (+)‐muconolactone and β‐oxoadipic acid. The salicylate hydroxylase required NADH or NADPH as cofactor and 1 mole of O2was taken up and 1 mole of CO2evolved for each mole of salicylate oxidized. Catalytic quantities of flavine adenine dinucleotide (FAD) but not flavine mononucleotide (FMN) activated the enzyme. Thecis‐cis‐muconate lactonizing enzyme was activated by Mn2+and inhib

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1971.tb01006.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

SUMMARY:The responses of various groups of Gram negative, yellow pigmented rods constructed on the basis of classical criteria to a wide range of cultural and biochemical features were examined. The occurrence of a wide area of slime surrounding true growth with isolates which may spread but did not glide is described. The correlation between the classical groups and divisions suggested on the basis of pigment absorption curves was examined.

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1971.tb01007.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

SUMMARY:Of 4 methods of assessing the bacteriological quality of mussels the semiquantitative method of the Fishmongers’Company was the most useful from the public health aspect where facilities may be limited. A modification of Clegg&Sherwood's method, using pour plates, was more sensitive and more simple to perform than the original method and is to be recommended for routine use. TwoEscherichia colicells/ml of mussel tissue probably represents the upper acceptable limit for satisfactory grading of mussels. The presence of small numbers of salmonellae in purified mussels is probably not highly significant but tests for their presence will yield useful epidemiological dat

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1971.tb01008.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

SUMMARY:Nine hundred and fiftyseven strains of yeasts were isolated from fresh marketed strawberries over 2 seasons. There werec.105viable yeasts/g of strawberry: many more were isolated at 5° than at 25°. Most of the yeasts belonged to the genusCryptococcus. Every yeast colony counted was subjected to identification tests selected by means of a computer‐made key, and identifications were done chiefly by semiquantitative aerobic growth tests combined with fermentation tests and microscopical examination of vegetative ce

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1971.tb01009.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

SUMMARY:Two testing procedures, B.S. 3286: 1960 and Kelsey&Sykes (1969), have been examined in terms of their reproducibility and ease of operation using different types of disinfectants. The capacity use‐dilution test realistically simulated practical conditions and gave reasonable results. It is recommended for use‐dilution assessments of general purpose disinfecta

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1971.tb01010.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

SUMMARY:The antitubercular effect of disinfectants has been tested with adaptations of 2 recently introduced techniques (Kelsey&Sykes, 1969; Bergan&Lystad, 1971b). Only the phenolic compounds tested were effective tuberculocides.

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1971.tb01011.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

SUMMARY:Treatment with formaldehyde during laundering conferred a persistent residual disinfectant action on wool blankets but not on cotton ones. The former was shown by a slower rate of bacterial contamination of blankets during use or by the more rapid disappearance of nonsporing bacteria from contaminated blankets during storage. The formaldehyde treated blankets had no perceptible odour during use, and did not cause irritation of the skin or mucous membranes. It is suggested that the formaldehyde treatment of wool blankets might be worthy of trial as a means of reducing bacterial contamination in rooms occupied by patients with a high risk of infection.

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1971.tb01012.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

SUMMARY:Sodium deoxycholate (0.2%) inhibited growth ofEscherichia coliin semi‐digested meat and milk, but had very little effect in media in which the source of nitrogen was amino acids or ammonia. The addition of more digested forms of protein (peptone and casein hydrolysate) to a less digested form (beef infusion) overcame the inhibitory effect. The significance which this phenomenon may have in controlling the population ofE. coliin the small intestine is discusse

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1971.tb01013.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

SUMMARY:The survival ofEscherichia coliand spores ofBacillus subtilisvar.nigerandB. stearothermophilussuspended in halocarbon propellents for several weeks has been demonstrated. There was no significant loss of dipicolinic acid from the spores. Lethal action may be due to interaction of the propellents with cellular lipid.

ISSN:0021-8847    

DOI:10.1111/j.1365-2672.1971.tb01014.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY