ISSN:0077-8923    

DOI:10.1111/j.1749-6632.1987.tb36232.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:0077-8923    

DOI:10.1111/j.1749-6632.1987.tb36233.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SUMMARYThe glycosylated and the non‐glycosylated recombinant human granulocyte‐macrophage colony‐stimulating factor (rh GM‐CSF) expressed in Chinese hamster ovary cells andE. coli, respectively, were administered in rhesus monkeys either by the subcutaneous (three times daily) or intravenous route (6‐hr infusions) for seven consecutive days. Within 24 hr peripheral white blood cells (WBC) increased 2‐3‐fold over normal values. Thereafter, the WBC increased steadily in a dose‐dependent manner to reach maximum levels on the last day of or one day after the treatment period. The differential counts showed that neutrophils contributed to 50‐80%, eosinophils to 10‐20%, monocytes to 2‐7%, and lymphocytes to 15–30% of the WBC rise. No effect was found on platelets and erythrocytes. After termination of treatment, WBC counts returned to normal levels within one week. Subcutaneously administered CSF was more effective in inducing leukocytosis than that injected intravenously. In addition to the rise in WBC, the administered rh GM‐CSF also enhanced the oxidative metabolism and bactericidal activity of the circulating mature granulocytes isolated from the blood of monkeys treated with rh GM‐CSF. These results show that glycosylated or non‐glycosylated rh GM‐CSF is both an effective stimulator of leukocytosis and a potent activator of the functional activit

ISSN:0077-8923    

DOI:10.1111/j.1749-6632.1987.tb36234.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SUMMARYThe conditioned media of 34 human tumor cell lines were screened for the ability to induce granulocyte‐macrophage coloniesin vitroin bone marrow cultures, to stimulate proliferation of a murine IL‐3 dependent hemopoietic cell line (32D clone 3) and to stimulate thymidine incorporation in suspension cultures of acute myelogenous leukemia cells. Twelve tumor cell lines produced factors that were active in these assays. The conditioned medium of the glioblastoma cell line U87 MG was characterized in detail and found to contain G‐CSF and GM‐CSF. Cloning and sequencing of the U87 MG G‐CSF indicated that it was derived from G‐CSF b mRNA, which encodes a protein with a deletion of 3 amino acids at residues 36–38. The gene for G‐CSF was mapped to human chromosome 17 band q21, a region involved in translocations frequently found in acute promyelocytic leukemia. G‐CSF (U87MG) was able to induce granulocytic differentiation of the total population of a murine IL‐3 dependent cell line, 32D clone 3; this effect was antagonized by IL‐3. GM‐CSF (U87‐MG) supported the proliferation without inducing differentiation of two growth factor‐dependent leukemic cell

ISSN:0077-8923    

DOI:10.1111/j.1749-6632.1987.tb36235.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SUMMARYAlthough mechanisms controlling differentiation of hemopoietic stem and early progenitor cells are still poorly understood, it is generally conceded that a pivotal role is played by hemopoietic growth factors (HGFs).1,2However,in‐vitroanalysis of their action on early progenitors may be obscured by cell‐cell interaction, as well as by the presence of fetal bovine serutn (FBS). To overcome these limitations, we investigated the action of pure multipotent or lineage‐specific HGFs on purified progenitors grown in FBS‐free cultures.In themurinesystem, highly purified progenitors were cultured in the presence of multipotent colony‐stimulating factor (multi‐CSF, also termed interleukin‐3), erythropoietin (Ep) and macrophagic‐CSF (M‐CSF). Each HGF was unable by itself to induce significant colony growth. However, combined addition of multi‐CSF and either Ep or M‐CSF gave rise only to pure erythroid or macrophagic colonies, respectively.Partly purifiedhumanprogenitors were challenged by human granulomonocytic‐CSF (GM‐CSF), pluripotent CSF (PPO, also termed granulocytic‐CSF, G‐CSF) and Ep. Here again, each HGF was unableper seto promote colony growth, but combined addition of GM‐CSF or PPO and Ep gave rise only to pure erythroid colonies.These results support a model of early hemopoietic differentiation according to which multi‐lineage HGFs represent “competence” GFs, the action of which is complemented b

ISSN:0077-8923    

DOI:10.1111/j.1749-6632.1987.tb36236.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:0077-8923    

DOI:10.1111/j.1749-6632.1987.tb36237.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Cell adhesion to the extracellular matrix plays an important role in the complex phenomena involving cell motility, such as tumor metastasis1and embryonic development.2Interaction with the extracellular matrix is also required for the proper cellular response to growth and differentiation factors.3A number of extracellular glycoproteins, which include fibronectin, laminin, vitronectin and collagens, are able to protnote cell adhesion by interacting with the plasma membrane.4The complex and dynamic process of cell adhesion requires that, after binding to the adhesive factor, receptors in the plasma membrane either cluster and participate in the formation of adhesion plaques that are necessary for stable adhesion, or otherwise interact with the cytoskeletal motor to activate the tractional forces necessary for cell locomotion.5In the present paper we will briefiy summarize some of our past work and report some new data on the identification, structural characterization and functional role of a mouse membrane glycoprotein implicated in the adhesion of fibroblasts, hemopoietic cells and platelets to the extracellular matrix.

ISSN:0077-8923    

DOI:10.1111/j.1749-6632.1987.tb36238.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The cellular events involved in the formation of hemopoietic progenitors during early embryogenesis are critical for the lifelong function of the blood‐forming system if, as is supposed, these progenitors arise during ontogeny and thereafter maintain their stock by self renewal. Several aspects of these events have been unravelled in recent years. Basic data on the interactions between stem cells and hemopoietic microenvironments have emerged from studies of the avian embryo by experimental approaches that are possible only in that class at the time of organogenesis. Interestingly, when it has been possible to devise appropriate experimental models, similar mechanisms have often been detected in mammals.When early developmental processes are investigated, difficulties arise from the small contingent of cells available. Cell markers have made it possible to trace individual cells and thus to follow lineages through their phenotypic changes and their extensive migrations. In the avian embryo, a cell‐marking technique devised by N. Le Douarin in 19691has become widely used for the study of various lineages. It consists in associating cells (or tissues, rudiments, embryonic territories) from two species, the chicken and the quail. Cell nuclei have a different appearance in these two species (Figure1). In recent years, the distinction of blood cells according to species in this heterospecific system has become refined by the availability of two monoclonal antibodies with affinity restricted to the quail species and which recognize the hemangioblastic lineage, MB12and QH13(Figure2). The hemangioblastic lineage comprises, on the one hand, endothelial cells, and on the other, blood cells and their progenitors. The points to consider about the ontogeny of the blood system are the relationships between stem cells and hemopoietic organ rudiments, the spatial and chronological origins of stem cells during ontogeny, the mechanisms through which they settle into the stromas of hemopoietic organs and the nature of the relationship between endothelial and hemopoietic ce

ISSN:0077-8923    

DOI:10.1111/j.1749-6632.1987.tb36239.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:0077-8923    

DOI:10.1111/j.1749-6632.1987.tb36240.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:0077-8923    

DOI:10.1111/j.1749-6632.1987.tb36241.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY