摘要:

The extremely rapid growth in the field of protein chemistry during the past 50 years has been due in large part to the development of very effective techniques of protein separation and analysis. A number of techniques such as electrophoresis or isoelectric focusing, based on the overall charge and mass of a biomolecule, have been widely applied. However, preparative isolation of proteins often involves some form of chromatography. The two modes of chromatography that have been most widely accepted and used for separation of biomolecules are partition chromatography and adsorption chromatography which includes affinity chromatography. The technique of affinity chromatography exploits the unique biological specificity inherent in a ligand-macro-molecule interaction. Thus, among separation procedures, a group of methods based on the molecular affinities found in biological systems have become increasingly important.

ISSN:0007-8980    

DOI:10.1080/10408348708542800

出版商:Taylor & Francis Group    

年代:1987

数据来源: Taylor

摘要:

Inductively coupled plasmas (ICPs) operated at atmospheric pressure are commonly used as vaporization-atomization-excitation-ionization sources in analytical atomic emission spectrometry (AES), atomic fluorescence spectrometry (AFS), and mass spectrometry (MS). Argon-supported ICP discharges exhibit several advantages for spectrochemical analysis. They provide high powers of detection and wide concentration dynamic ranges, with minimal matrix effects, and they are extremely useful for simultaneous multielement determinations. Because of ease of operation and a power requirement lower than that for other support gases such as air, nitrogen, oxygen, and hydrogen, most laboratory ICP discharges are sustained in pure argon. This relative ease of operation and reduced power requirement are partly attributed to lower thermal conductivity, lower heat capacity, and lower electrical resistivity of Ar, compared to other support gases.

ISSN:0007-8980    

DOI:10.1080/10408348708542801

出版商:Taylor & Francis Group    

年代:1987

数据来源: Taylor