摘要:

We describe chemical dehydration with 2,2-dimethoxypropane (DMP) for rapid paraffin embedding using a mixture of DMP and mineral oil followed by mineral oil as clearing intermediates. This method is useful for classical histological techniques as well as for histochemistry and immunocytochemistry.

ISSN:1052-0295    

DOI:10.3109/10520299409106304

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

Silica bodies are discrete deposits of dehydrated silica within epidermal cells. To describe these bodies completely, surrounding organic and unsilicified material must be removed. Methods generally used for isolating and preparing silica bodies were unsuitable for most grass species. An effective method for studying grasses is described here. After ashing the plant tissue, the ash was repeatedly rinsed with HCl in a specialized multiple funnel manifold and collected on Nuclepore filters. In addition, the silica bodies were sonicated for a few minutes to remove any remaining mineral impurities. Compared to conventional procedures, this method has a number of advantages: unsilicified material and mineral impurities were removed effectively, smaller quantities of plant tissue could be used, and the loss of silica bodies was minimized.

ISSN:1052-0295    

DOI:10.3109/10520299409106305

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

An improved methenamine-silver impregnation method is presented which exhibits sensitivity for amyloid substances comparable to that of anti-βprotein immunostaining. In optimally treated sections, this technique stained bothβ-amyloid deposits and neurofibrillary tangles, which are known to have aβ-pleated structure. This simple procedure allows a large number of sections to be stained for routine examination.

ISSN:1052-0295    

DOI:10.3109/10520299409106306

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

ISSN:1052-0295    

DOI:10.3109/10520299409106307

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

ISSN:1052-0295    

DOI:10.3109/10520299409106308

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

ISSN:1052-0295    

DOI:10.3109/10520299409106309

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor