摘要:

Various procedures suitable for routine in situ embedding of cell monolayers were tested including: (1) the use of different Epon substitutes, (2) the use of different types of plas-ticware obtained from different sources, and (3) different methods of preparing capsules for sectioning. Different resins reacted differently with different plastics and type of preparation. Merck Epon substitute bound to most of the plastics tested. Ladd Epon substitute released cleanly from all plastics tested when a suitable method of preparation was used. The results show that for routine embedding of cell monolayers it is necessary to select an appropriate Epon substitute and method of preparation of capsules for the type of plasticware used. A routine method is described, with various alternative steps which can be applied when particular difficulties are encountered.

ISSN:1052-0295    

DOI:10.3109/10520299109109985

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

A method is described for obtaining thin (1μm) sections for light microscopy from large area thick (100μm) sections of low viscosity nitrocellulose embedded specimens of human spinal osteoligamentous material.

ISSN:1052-0295    

DOI:10.3109/10520299109109986

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

ISSN:1052-0295    

DOI:10.3109/10520299109109987

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor