摘要:

We determined the effects of rapid freezing and perfusion fixation on fiber geometry and capillarity in rat skeletal muscle. Fiber areas were significantly decreased, and capillary densities significantly increased, in perfusion-fixed versus quick-frozen muscle. Significant differences in capillary-to-fiber ratios were not observed, suggesting that differences in fiber geometry, not the methods of quantifying capillaries, accounted for the differences in capillary density. We conclude that estimates of fiber geometry, capillarity, and diffusive gas conductances obtained from perfusion-fixed muscles are subject to significant error due to shrinkage.

ISSN:1052-0295    

DOI:10.3109/10520299209110042

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

M. A. Hayat (ed.)Colloidal Gold Principles, Methods and ApplicationsAcademic Press, Inc., San Diego, CA, 1989, 1991.

ISSN:1052-0295    

DOI:10.3109/10520299209110043

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

ISSN:1052-0295    

DOI:10.3109/10520299209110044

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor