摘要:

Polyethylene glycol (400) with 90% glycerol (aqueous) is introduced as an efficient solvent system for lipid stains. Various lipid-soluble dyes were dissolved in this solvent system and tested for their intensity, contrast, and specificity of staining of suberin lamellae in plant tissue. The stability (i.e., lack of precipitation) of the various staining solutions in the presence of fresh tissue was also tested. When dissolved in polyethylene glycol-glycerol, Sudan red 7B (fat red) was the best nonfluorescent stain and fluorol yellow 088 (solvent green 4) was an excellent fluorochrome. These two dyes formed stable staining solutions which efficiently stained lipids in fresh sections without forming precipitates. Estimations of the solubilities of these dyes in the solvent compared with their solubilities in lipids of various chemical types indicated that they should both be effective stains for lipids in general.

ISSN:1052-0295    

DOI:10.3109/10520299109110562

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

A procedure was developed which prevents wrinkles in autoradiographic emulsion when sections, embedded in glycol methacrylate, are stained with hematoxylin and eosin-phloxine. Craniofacial tissues labeled with tritiated thymidine were collected and mounted on slides. Slides were dipped in emulsion, stored for one month and developed. The slides were immersed in liquefied celloidin and subsequently stained with a modified hematoxylin and eosin-phloxine procedure. Results showed that the emulsion did not wrinkle and the procedure did not effect the occurrence of labeled cells.

ISSN:1052-0295    

DOI:10.3109/10520299109110563

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

A quick embedding method employing UV polymerization reactions has been devised for embedding fibers in acrylic and meth-acrylate media. The resultant thin, flat embed-dings are suitable for both light and electron microscopy.

ISSN:1052-0295    

DOI:10.3109/10520299109110564

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

The improved method presented here for localizing monophenoloxidase activity of tyrosinase (E.C. 1.14.18.1.) after electrophoresis is based on the transfer of electrons from the monophenolic substrate, tyrosine methyl ester, to an artificial acceptor, phenazine methosulfate, and subsequent reduction of nitro blue tetrazo-lium into a violet formazan. This method is rapid, sensitive and versatile compared to the standard method. The electron transferred from mono-phenol can be accepted directly by nitro blue tetrazolium; although the background of the gel is clear, the sensitivity is decreased. The mono-phenol-PMS-NBT method is suitable for both plant and animal samples. This method can also be used for histochemical demonstration of monophenoloxidase activity.

ISSN:1052-0295    

DOI:10.3109/10520299109110565

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

Silver impregnation with silver-protein compounds is widely used for staining tissue sections and cell cultures. Some authors report that the results obtained with these methods have not always been reproducible because the reagent's composition varies according to the manufacturer. To avoid this problem in the method described in this paper, a silver proteinate, produced in our own laboratory is used. Although our method is based on Bodian's, the modifications we have made allows its use for both free-living cells (protozoa) and cells grown in culture (nerve cells). The significant modifications are 1) different fixation, 2) postfixation with Cajal's for-mol-bromide, 3) changes in the duration of the impregnation steps technique and 4) elimination of metallic copper. The method reported here enables us to use silver proteinate whenever we require it and to control the composition of the silver proteinate. This technique can be used for cells cultured in either plastic or glass.

ISSN:1052-0295    

DOI:10.3109/10520299109110566

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

A simple technique for placing reference markers on serial sections is presented. The objective is to permit proper cut orientation, especially when the shape of a structure appearing in the various cuts is to be digitized for computer analysis.

ISSN:1052-0295    

DOI:10.3109/10520299109110567

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

After treatment of epoxy resin semithin sections from glutaraldehyde fixed rat large intestine with 5% aqueous phosphotungstic acid (PTA), staining with unpurified 0.2% solutions of methyl green at 60 C for 5 min produces a color differentiation between mucin granules of goblet cells. Some mucin granules and the glycocalyx appear deep green while the remaining granules, luminal mucin and collagen fibers are pink. The known contamination of unpurified methyl green with crystal violet seems to be responsible for the pink staining reaction of the latter structures, which also present an orange-red fluorescence under green exciting light. Electron microscopic observations show selective contrast of mucin granules which appear with a different amount of PTA deposits. This procedure is useful to reveal the heterogeneity of mucin granules in light and electron microscopy.

ISSN:1052-0295    

DOI:10.3109/10520299109110568

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

For many years, a variant of the silver impregnation technique of Bielchowsky has been used to study the lymph node because it clearly outlines the various structures which are usually hard to contrast with standard staining methods. Like other variants of silver impregnation, this method blackens the cell nuclei as well as the reticular fibers; however, it inhibits the impregnation of the nuclear chromatin immediately adjacent to fibers. Hence, this variant selectively darkens the lymphoid cell populations of the nodal structures which contain a loose fiber network.To study the blood vascular network of the lymph node based on perfusion of colloidal carbon, a staining procedure was needed which would contrast nodal structures on thick sections, while allowing the carbon-filled small blood vessels to be distinguished from the impregnated coarse reticular fibers. In an attempt to adapt this variant of Bielchowsky's technique, 10, 20, 40 and 60nmthick sections from rat nodes, fixed in a solution of Bouin-Hollande for 72 hr, were silver impregnated with serial dilutions (1:2 to 1:128) of the ammoniacal silver solution. Forty-micrometer thick sections impregnated with a 1:16 dilution of the original silver solution at 37 C and for 30 min provided the best results for the conditions.

ISSN:1052-0295    

DOI:10.3109/10520299109110569

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

A two-sided double labeling method using protein A gold was used to demonstrate the presence of two hormones within the same anterior pituitary cell granule. A single probe size was used for both section faces but one side of the grid was silver enhanced. The use of a single probe size reduced the cost of the study and eliminated the variations in labeling efficiency that result from the use of different probe sizes.

ISSN:1052-0295    

DOI:10.3109/10520299109110570

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

NASA-approved hardware was designed for an experiment involving in-flight fixation of 4.8-day-old seedlings ofBrassica perviri-dis.The hardware is inexpensive, reliable, and simple to construct. This hardware aboard flight 61-C of space shuttleColumbiafunctioned perfectly, and required less than 0.5 hr of crew time for training. Analyses of cellular ultrastructure indicated excellent ultrastructural preservation.

ISSN:1052-0295    

DOI:10.3109/10520299109110571

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor