摘要:

Lymphoid tissue, and/or isolated peripheral mononuclear blood cells were fixed in acid ethanol and embedded in polyester wax (melting point 37 C). The excellent cytomorphol-ogy obtained allowed distinguishing different types of individual lymphoid and nonlymphoid cells. Furthermore, this procedure was satisfactory in the immunophenotyping of histiocytes, endothelial, mesenchymal, epithelial cells, different (sub-) types of lymphocytes and also of lym-phokine activated killer (LAK) cells. The staining patterns obtained with the different poly- and monoclonal antibodies on polyester wax sections were not only analogous to those obtained on frozen sections, but cells which had incorporated bromodeoxyuridine could be double labeled with specific antiserum.

ISSN:1052-0295    

DOI:10.3109/10520299109110551

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

A method is described for preparing the same cell from a cytospin preparation for comparative investigation by light microscopy, scanning electron microscopy and transmission electron microscopy. A permanent numbered grid pattern was etched on a glass microscope slide to facilitate cell location in each microscopic mode. Data from one cell or group of cells was thus obtained from three sources. This method provides a useful adjunct to routine cytological diagnosis.

ISSN:1052-0295    

DOI:10.3109/10520299109110552

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

A technique is described for selectively silver staining nucleoli, active nucleolus organizers, nucleolar material attached to chromosomes, kinetochores, synaptonemal complexes, and chromosome cores in plant cells. The technique, called salt-nylon silver staining, involves spreading cells on glass slides, treating the cells with a solution of saline sodium citrate, and incubating the cells in a silver nitrate solution covered with nylon screen. Selected variables important for achieving reliable silver staining are considered.

ISSN:1052-0295    

DOI:10.3109/10520299109110553

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

The microwave stimulated immunodetection of a tumor associated antigen (TAG-12) by monoclonal antibody 7A9 and an avidin-biotinylated alkaline phosphatase kit was compared with the conventional staining method. No difference in the staining pattern of antibody 7A9 was noticed in serial paraffin sections of 50 specimens including normal, benign and malignant breast tissues after microwave irradiated and conventional immunostaining. The results demonstrate that microwave stimulated immunostaining gives reliable results and can remarkably reduce the time of the staining procedure.

ISSN:1052-0295    

DOI:10.3109/10520299109110554

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

An improved and time reducing method is presented for the histological evaluation of bone containing polymethylmethacrylate (PMMA) bone cement. The undecalcified bone was embedded in epoxy resin and sections of 50-100μm thickness were produced using a commercially available cutting grinding system. The sections were stained with Stevenel's blue and van Gieson picrofuchsin or a modified hematoxylin-eosin. PMMA bone cement was not dissolved and remained enabling examination in situ of an intact cement bone interface and tissue reaction without decalcification.

ISSN:1052-0295    

DOI:10.3109/10520299109110555

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

Low temperature photochemical silver staining was used for immunohistochemical demonstration of epidermal growth factor (EGF) in the rat submandibular gland. EGF was shown to be clearly localized in granular convoluted tubule cells. Silver staining with an immu-nogold method showed excellent resolution of the antigen localization.

ISSN:1052-0295    

DOI:10.3109/10520299109110556

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

Two simple methods are described for flat embedding of sections cut from paraffin blocks of brain tissue in the hydrophilic resin LR White. The ability of fresh resin to polymerize when added to already cured resin is exploited. In the first method, a tissue section with a drop of LR White is pressed to the bottom of a gelatin capsule using a blank block. In the second method, the section in a drop of resin is sandwiched between the sawed halves of a blank block. After curing, thin sections containing large areas of tissue can be collected and processed for immunocytochemistr y.

ISSN:1052-0295    

DOI:10.3109/10520299109110557

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

Otic bullas of the rat, obtained by excision and formalin fixed, are successfully embedded in methylmethacrylate by dehydration and subsequent infiltration with plastic under vacuum. Sections 10μm thick are obtained by cutting the trimmed and sandpapered acrylic blocks on an LKB multirange microtome. The sections are collected on adhesive tape and stained with a Trichrome stain (modified Weigert-van Gieson). Finally, the sections attached to the tape are mounted on microscope slides with glycerin-gelatin and sealed in the same medium. Serial sections are used for three-dimensional graphic reconstruction.

ISSN:1052-0295    

DOI:10.3109/10520299109110558

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

A simple modification of cytochrome oxidase histochemistry was undertaken to prevent the artifactual condensation of reaction product. The quality and reliability of the histochemical method were greatly improved by mounting the tissue after reaction.

ISSN:1052-0295    

DOI:10.3109/10520299109110559

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

A procedure is described which allows for rapid detection of cell surface binding or cytoskeleton binding monoclonal antibodies. At the same time this procedure ensures that selected antibodies will be useful in Western blot analysis. This procedure including the cytochemistry and Western blot analysis requires only 100μl of supernatant and can be done directly from the original 96 well plates into which the fusion was plated. One person can easily assay several hundred supernatants in one day for the ability to stain both cells and selected proteins in Western blot analysis.

ISSN:1052-0295    

DOI:10.3109/10520299109110560

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor