摘要:

A practical and reliable staining procedure was developed to distinguish the viability and acrosomal status of bull, boar and rabbit spermatozoa. The first stain with trypan blue or Congo red is rapid and avoids artifacts. This stain is precipitated by neutral red during the 2 min required for fixation. The precipitate gives a high contrast black color, resistant to the subsequent rinsings and persists during the time required for staining the acrosome with Giemsa. Ten classes of spermatozoa are distinguished (live or dead with intact acrosomes, loose acrosomes, damaged acrosomes, no acrosome, or with no acrosome and no postacrosomal ring). The intact acrosomes are purple, the loose acrosomes are dark lavender and the damaged acrosomes are pale lavender. The anterior part of the head of live spermatozoa with no acrosome is white or light pink and the same area of dead spermatozoa is white or pale gray. The postacrosomal ring is red. The postacrosomal area of the head of live spermatozoa is white or light pink and the same part of dead spermatozoa is black, dark violet or gray. The procedure did not give satisfactory results for stallion spermatozoa.

ISSN:1052-0295    

DOI:10.3109/10520299209110020

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

We compared three different staining methods to determine if the dermal elastic fiber content of the HRS/Skh-1 hairless mouse could be accurately measured by color image analysis. Comparisons were made among Klig-man's modification of Luna's mast cell stain for elastin, Unna's orcein stain with or without potassium permanganate preoxidation, and Gomori's aldehyde fuchsin stain with potassium permanganate preoxidation. The color image analysis system could be used to identify and quantify murine dermal elastin fibers in sections stained by all three methods. Gomori's aldehyde fuchsin stain with preoxidation demonstrated twice the content of dermal elastic fibers demonstrated by either Kligman's modification of Luna's mast cell stain or Unna's orcein stain with or without preoxidation. Gomori's aldehyde fuchsin method with preoxidation should be considered the stain of choice for evaluating murine dermal elastic fiber content.

ISSN:1052-0295    

DOI:10.3109/10520299209110021

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

The binding of histamine to cultured microvascular endothelial cells and glycol methacrylate embedded ovarian tissue sections has been localized using fluorescein-albumin-histamine conjugate. Histamine conjugate was bound to the plasma membranes and nuclei of luteal, endothelial, and ovarian stromal cells. An apparent increase in the binding of histamine to nuclei was observed in the presence of cimetidine but the plasma membrane staining was still evident. Unlike cimetidine, pyrilamine completely inhibited the binding of histamine to the plasma membrane. Instead, in the presence of pyrilamine, histamine bound exclusively to the nuclei of endothelial, germinal epithelial, granulosa, and stromal cells. However, the nuclei of terminally differentiated luteal cells and oocytes were not labeled. The functional significance of these nuclear histamine binding sites remains to be determined.

ISSN:1052-0295    

DOI:10.3109/10520299209110022

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

Paraffin wax embedding methods suitable for demonstrating the distribution of enzyme activity in tissues sections are uncommon; most procedures rely on the use of frozen section techniques. This paper describes a system for demonstrating certain enzymes which involves incubation of the tissue with appropriate substrates before a Paramat wax embedding procedure. While it has distinct merits of its own, the procedure is eminently suitable for use where a cryostat is not available; it can also be readily applied to other enzymes and tissues.

ISSN:1052-0295    

DOI:10.3109/10520299209110023

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

Pneumocystis cariniiinfected rat lungs were postfixed with a mixture of OsO4and K4Fe(CN)6. A marked improvement in staining of cell membranes, endoplasmic reticulum, nuclear membranes and glycogen was observed. These improvements were seen in both the trophic and cystic forms of the organisms. The addition of K4Fe(CN)6did not improve the staining of cell walls, microtubules or ribosomes. Trophozoites were seen attached to both type 1 and type 2 pneumocytes by filopodia and/or intercalation of the cell body ofP. cariniiwith the host lung cells. It is expected that the improvement in ultrastructural detail will allow better understanding of the ultrastructure ofP. cariniiand provide insights into the modes of action of various antimicrobial compounds on this organism.

ISSN:1052-0295    

DOI:10.3109/10520299209110024

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

A simple silver staining technique was applied to serial ultrathin sections ofVicia fabaroot tip meristems to investigate the structural organization of the nucleoli. The procedure consists of three steps: hydrophilic Lowicryl resin embedding, preparation of ribbons of serial ultrathin sections, and silver impregnation. This technique provided good contrast for a filamentous structure, previously called the“nucleolonema”, in nucleoli at the light microscope level. Silver appears to react with some acidic proteins involved with ribosomal RNA transcription, since the major constituents of the nucleolonema are believed to be active rRNA transcriptional units. Tracing the nucleolonema through serial ultrathin sections strongly suggests that the nucleolonema is meandering and partly coiling within the nucleolus. The present technique permits identification of the nucleolonema in other plant species and investigation of its three-dimensional structural organization.

ISSN:1052-0295    

DOI:10.3109/10520299209110025

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

A plastic embedding technique employing fluorescently labelled dextran-amines is described. After application of tracer to cut nerves and appropriate transport time, animals were fixed in paraformaldehyde. Subsequently their brains were dissected, heads and brains were dehydrated, embedded in methacrylate and sectioned serially on a rotary microtome. Plastic sections allow high resolution of single neuron profiles and complete serial reconstruction of un-distorted sections, including embryos with large amounts of yolk. In conjunction with whole mount analysis and double labelling, this technique can accurately reveal the spatial relationships of nerve components throughout development.

ISSN:1052-0295    

DOI:10.3109/10520299209110026

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

A new histochemical reaction for the identification of histone type basic proteins has been developed. Carbonyldiimidazol is used to activate the basic proteins of TCA-extracted nuclei, their m-aminophenylboronic acid complex is prepared, and the DNA-free, histone-containing nuclei are stained with toluidine blue at pH 5.5.

ISSN:1052-0295    

DOI:10.3109/10520299209110027

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

Anti-DNA polymerase a and Ki-67 are monoclonal antibodies that recognize nuclear antigens expressed in proliferating cells. In this study, we evaluated various methods of embedding and fixing brain tumor specimens to optimize staining with these antibodies. In fresh frozen sections, postfixation with 4% paraformaldehyde, 100% methanol, 95% ethanol and 10% buffered formalin were tested; also tested were prefixation with 4% paraformaldehyde followed by freezing and fixation with 100% methanol, 95% ethanol, or 10% buffered formalin followed by embedding in paraffin. For both antibodies, postfixation of fresh frozen sections with 4% paraformaldehyde at 4 C gave the most intense staining and lowest background activity while preserving histological features. This technique can be used in routine clinical practice to predict the growth potential of tumors.

ISSN:1052-0295    

DOI:10.3109/10520299209110028

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

In situations where there is a need to minimize sampling error or sample size, the coefficient of variation (CV) may be used to evaluate sampling error as a function of the number of observations or subjects in a sample. For example, CV is useful for estimating the minimum number of electron micrographs (Nmin) required to obtain a representative field sample for stereological analysis. To facilitate the determination of Nmin, we have written a program (COEFficient) for DOS microcomputers which calculates CVs. COEF assists the user in reducing error to that which solely reflects biological variability, thereby minimizing the time and cost of subsequent analyses.

ISSN:1052-0295    

DOI:10.3109/10520299209110029

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor