ISSN:1052-0295    

DOI:10.3109/10520299209110045

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

A new modification of the tetrachrome method for bone osteoid in paraffin sections has been designed. The modified tetrachrome method suitable for routine use in any histology laboratory retains the simplicity of the original method and gives good results on the freshly fixed, decalcified, paraffin embedded material. Osteoid tissue is stained deep blue and normally mineralized bone is stained red. Defectively mineralized bone stains pale blue or pink and the cellular population is clearly identifiable. The ability to distinguish the osteoid tissue from mineralized bone and connective tissue and cartilage makes diagnosis of osteomalacia or osteoid producing tumors or assessment of ossification process straightforward, without the need for un-decalcified sections. By displaying simultaneously irregularities in the mineralized matrix and morphology of bone cells, the method also permits the diagnosis of conditions recently described in patients with osteoporotic fractures, such as osteocytic degeneration and bone tissue defects.

ISSN:1052-0295    

DOI:10.3109/10520299209110046

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

4′, 6-Diamidino-2-phenylindole hydrochloride (DAPI) is a fluorescent dye with high affinity for DNA. We have employed it as a fluorescent chromatin counterstain on sections immunofluorescent-stained using rhodamine and on tissues enzymatically stained usingβ-galactosidase. DAPI also allows easy identification of mitotic figures and can be used to supplement cytochemical studies involving cell division in the nervous system.

ISSN:1052-0295    

DOI:10.3109/10520299209110047

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

The influence of vital staining with trypan blue or fluorescein diacetate on the fertilization of mouse oocytes and the developmental potential of mouse embryos was assessed. Neither stain induced spontaneous activation in mouse oocytes, nor did they impair the in vitro development and implantation of mouse zygotes, two-cell embryos, stressed morulae or blastocysts. However, fertilization and subsequent development of mouse oocytes have been shown to be reduced by vital staining.

ISSN:1052-0295    

DOI:10.3109/10520299209110048

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

A rapid three-step DAPI technique is proposed for detecting meiotic stages and sperm head evolution in yolky, fertilized stick insect eggs, which were difficult to analyze with other methods. Fixed eggs were freed from chorionic envelopes and stained directly in DAPI/PBS solution. After rinsing, eggs were singly squashed in a drop of mounting buffer and examined under a microscope with incident fluorescent illumination. The method was almost uniformly successful, and direct observation of nuclear structures, coupled with fluorometry, allowed easy recognition of bivalents, diads, pronuclei and their DNA content. The DAPI method proposed here appears particularly helpful for investigating unusual reproductive modes in eggs with large amounts of yolk.

ISSN:1052-0295    

DOI:10.3109/10520299209110049

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

Celloidin stabilized pre-epithelial mucus gel (PMG) is stained in cryostat sections of the rat colon for light microscopic lectin histochemistry. Specific sugar residues of the mucins are demonstrated both in the PMG and in the mucin-containing cells of the mucosa.

ISSN:1052-0295    

DOI:10.3109/10520299209110050

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

A new double-embedding technique for thin tissue membranes is presented. This technique is useful for thin membranes such as mesenteric membranes from rodents, which usually measure only 10μm in thickness. Several membranes are fixed and mounted on four needles located at the bottom of a plastic box. The box is filled with agarose at 50 C and then allowed to solidify. The agarose block is then removed, dehydrated in alcohol, cleared with Histo Petrol (isoparaffin hydrocarbons), permeated with paraffin and sectioned. The morphology is comparable to that obtained with methacrylate plastic embedding but is less time-consuming, less hazardous since no plastic hardener and activator are used and makes immunohistochemical studies easier.

ISSN:1052-0295    

DOI:10.3109/10520299209110051

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

It has been suggested that the use of avidin-biotin immunohistochemical techniques for antigen detection in neural tissue produces nonspecific background staining. For this reason neural tissue was used to test the quality, sensitivity and specificity of four commercially available antibody detection kits which use avidin or streptavidin binding to biotin. Free-floating, thick-section immunohistochemistry on perfusion fixed rat central nervous system revealed variability among staining kits for all parameters analyzed under the same experimental conditions. The reagents from the Vector‘Elite’kit were the most sensitive and specific, and received the highest overall rating for quality. Most commercial products tested could be used at greater dilutions than those recommended by the manufacturers without compromising specific staining. No staining was evident when the primary and secondary antibodies were omitted. This suggests that nonspecific binding is unlikely to be due to endogenous ligands, charge of hydrophilic reactions between these tertiary complexes and the tissue sections.

ISSN:1052-0295    

DOI:10.3109/10520299209110052

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

Lead tetraacetate-thiocarbohydrazide-silver proteinate reaction sequence for light microscopy of polysaccharides was evaluated on Carnoy's fixed rat liver sections. The results of this evaluation suggest that, on the light microscopic level, the lead tetraacetate-thiocarbohydrazide-silver proteinate method may serve as a practical and histochemically specific alternative to the lead tetraacetate-Schiff reaction for the localization of tissue carbohydrates.

ISSN:1052-0295    

DOI:10.3109/10520299209110053

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

A method is described for flat-embedding thin membranous tissues in Historesin. It allows easy orientation for sectioning large areas parallel to the surface. Selected fields can be monitored from the unfixed specimen, throughout preparation, to mounting on the microscope slide. For cross-sectioning, the flat-embedded tissue can be stacked and re-embedded to increase the amount of material examined per section.

ISSN:1052-0295    

DOI:10.3109/10520299209110054

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor