摘要:

Pure metal 4.4′,4″,4‴-tetxa-substituted, sulfo-, carboxy- and nitrophthalocyanines were synthesized. Mounted, deparaffinized and partially dehydrated sections of plant tissues were stained with 0.5% safranin in 50% alcohol for 5–10 min. Excess safranin was removed with a series of 70%, 95% and absolute alcohol washes. The sections were then stained for 2–3 min using metal 4,4′,4″,4‴-phthalocyanine tetracarboxylic acid (MPTC, 0.5% (V/V) containing a few drops of dilute sodium hydroxide), metal 4,4′,4″,4‴-tetra-sulfophthalocyanine (MPTS, 0.5% (V/V)) or metal tetranitrophthalocyanine (MPTN, 0.5% (V/V) in dimethyl sulfoxide). The sections were washed with 95%, then absolute alcohol; however, the metal tetranitrophthalocyanine section was washed only with absolute alcohol. Stained sections were treated briefly with xylene, then mounted on a coverslip. Bright peacock blue (MPTC and MPTS using Cu, Co or Ni), turquoise blue (MPTN using Cu or Ni) or parrot green (zinc phthalocyanine tetracarboxylic acid-ZnPTC, zinc phthalocyanine tetranitro derivative-ZnPTN) colors were obtained. Lignin-containing cells were stained red by safranin and the remaining cell structures were stained by the metal phthalocyanine complex with color brightness superior to that of fast green. Uniform staining, no color fading after a year, reliability, brief staining times, high color contrast (logϵ= 4.0-4.9) and ease of use make this double staining combination ideal for routine use and photomicrography.

ISSN:1052-0295    

DOI:10.3109/10520299309104681

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

Available mounting media cause fading of histological preparations over time. A study was designed to find the most suitable medium for durable mounting of Araldite embedded semithin sections of rabbit cerebral cortex stained with toluidine blue and pyronin G. Among four synthetic mounting media tested, only DePeX prevented fading of the sections during the first month. All mounting media tested helped preserve staining intensity after one month, since the fading rate after one year is only about half that in sections prepared without mounting medium. The average optical density of sections after one year was higher in preparations mounted with DePeX than in sections treated with the other mounting techniques tested in this study. After one year, the average optical density of sections mounted with DePeX had decreased approximately 20%.

ISSN:1052-0295    

DOI:10.3109/10520299309104682

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

We developed a modified in situ DNA-RNA hybridization technique and demonstrated c-myc proto-oncogene transcripts in nodular sclerosis type Hodgkin's disease (HD-NS). A hybridization probe was prepared by metabolic labelling of bromodeoxyuridine (BrdU). The hybridized probe was detected with anti-BrdU monoclonal antibody after incubation with ribonuclease H (RNase H), which specifically degrades RNA from DNA-RNA hybrids. In HD-NS, c-myc protooncogene transcripts were demonstrated in the cytoplasm of lacuna cells and a few surrounding lymphoid cells. This modified hybridization method was sensitive and applicable to the study of oncogene expression at a single cell level.

ISSN:1052-0295    

DOI:10.3109/10520299309104683

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

The air drying technique, widely used in animal cytogenetics, was adapted for use withZea maysL. chromosomes. Using a simple protocol without enzymatic maceration and avoiding the inconvenience of the squashing technique, good staining and C-banding were obtained from maize chromosome preparations.

ISSN:1052-0295    

DOI:10.3109/10520299309104684

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

Three stains, Hansel's stain, alkaline erythrocin B (AEB) and naphthalene black (NB), were used to demonstrate eosinophils in the urine of patients infected withSchistosoma haematobium. Hansel's stain was superior to the other two stains; it stained eosinophils bright red and their nuclei faint blue, and they were easily differentiated from neutrophils, lymphocytes, macrophages and epithelial cells. The method using AEB took longer than Hansel's stain and 10% of the specimens were lost during staining with this method. Like eosinophils, the neutrophils took up NB stain and their nuclei stained poorly with the counterstain.

ISSN:1052-0295    

DOI:10.3109/10520299309104685

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

A method to remove stainable cytoplasmic substances from cytogenetic preparation using RNase A treatment is reported. The preparations processed with this method are especially useful for the automated analysis of mi-cronuclei of cultured cells with cytochalasin B and of chromosome aberrations induced by radiation.

ISSN:1052-0295    

DOI:10.3109/10520299309104686

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

Nonisotopic methods of mRNA in situ hybridization have distinct advantages over isotopic techniques. Nonisotopically labeled probes are stable and nontoxic, have short detection times, demonstrate excellent spatial resolution of their signals and have sensitivities comparable to radiolabeled probes. We developed a simple method of generating nonisotopically labeled cRNA probes which is based on the reverse transcription polymerase chain reaction (RT-PCR) and used it to synthesize a panel of probes for various murine extracellular matrix genes. Engelbreth-Holm-Swarm (EHS) tumor RNA was reverse transcribed and PCR was used to amplify defined regions of multiple extracellular matrix protein genes from the resulting first strand cDNAs. Bacteriophage promoters which had been incorporated into the PCR products were then used to generate digoxigenin-conjugated anti-sense and sense cRNAs. The antisense probes were employed to detect the specific extracellular matrix protein mRNAs in the EHS tumor by in situ hybridization. This technique provides a rapid and efficient alternative to conventional transcription systems which use plasmid vectors for the synthesis of digoxigenin-labeled cRNA probes.

ISSN:1052-0295    

DOI:10.3109/10520299309104687

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

A technique is described for separating plant cells used for morphological studies. The plant material is placed in a concentrated solution of olive oil castile soap for 1–2 days or more. The material is then thoroughly washed and placed between two glass slides. The upper glass slide is lifted from the lower one, then gently pressed down several times. Through this procedure Malpighian cells of the seed coat ofSesbania punicea. mesophyll cells ofEuphorbia peplusand ofTrifolium pratenseand cortical cells of the aerial roots ofMonstera deliciosahave been separated. Various shapes of the Malpighian cells of the Sesbaniapuniceaseed coat can be observed along with intermediates.

ISSN:1052-0295    

DOI:10.3109/10520299309104688

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

A valuable method for specific localization of proanthocyanidins in plant tissues is given. The specificity is based on the acid hydrolysis of condensed tannins in situ via hot sulfuric acid, yielding bright red anthocyanidins. This treatment did not cause noticeable damage of tissue embedded in glycol methacrylate prior to sectioning. Further proof of specificity was achieved by control staining with the flavan-3–01-selective p-dimethylaminocinnamaldehyde (DMACA) reagent. In addition, comparison of adjacent sections stained with the DMACA reagent may give coarse information about the degree of polymerization of the proanthocyanidins.

ISSN:1052-0295    

DOI:10.3109/10520299309104689

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

A synthetic aromatic polymer has been used for preparing replicas of different microorganisms. This method of preparing highly concentrated (9.6 k) microbiological samples for scanning electron microscopy was compared with a standard method. The micrographs of the replicated samples are satisfactory. This method is rapid, cost effective and produces good results, especially in the case of spore-forming mycelial microorganisms.

ISSN:1052-0295    

DOI:10.3109/10520299309104690

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor