摘要:

Heating plant sections at 90 C with 0.5% aqueous ammonium oxalate is required to remove pectins. When applied to tissues rich in starch such as potato, this step produces heavy dextrinization of the starch which hinders subsequent evaluation of the extinction values of the cell walls. To overcome this a method has been devised to brush away the starch granules from the sections with a thin paint brush, just after paraffin removal by xylene. The slide is then processed as usual: pectins are removed by heat treatment, cell walls are stained with PAS and the stain intensity can be evaluated by photometry.

ISSN:1052-0295    

DOI:10.3109/10520299109109975

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

A new imprinting material,“Ther-mocool”, has been used for preparing negative replicas of plant as well as metal surfaces for SEM study. The technique has wide application and gives very good results.

ISSN:1052-0295    

DOI:10.3109/10520299109109976

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

Tartrate resistant acid phosphatase (TRAP) has been accepted as a marker for identification of osteoclasts. A method is reported here for quantitating TRAP using an image analysis system. The amount of the enzyme specific to osteoclasts can be used to differentiate osteoclasts from other cells capable of TRAP expression. TRAP expression characteristic of the osteoclast was compared with that of multinucleated giant cells (MNGC)s recruited to the site of subcutaneously implanted mineralized bone matrix. Two weeks post-implantation, the pellets were removed and processed for the demonstration of TRAP along with rat proximal tibiae. A large amount of TRAP was consistently expressed by the in situ osteoclasts. The MNGCs associated with the mineralized bone implants expressed little if any TRAP reaction product. Using this system, the amount of TRAP reaction product or any other enzyme reaction product expressed can be objectively and reproducibly quantitated.

ISSN:1052-0295    

DOI:10.3109/10520299109109977

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

This paper reports a technique using microwaves to assist penetration of stains into biopsy sections of muscle and peripheral nerve. The technique results in more consistent and reliable staining of tissue sections for examination by light microscopy.

ISSN:1052-0295    

DOI:10.3109/10520299109109978

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

When different antigens must be demonstrated in the same structure, the permanence of former antibodies can lead to false identification of another antigen. The peroxidase-antiperoxidase method was used, followed by the oxygen acceptor ethyl-carbazole. After staining the sections, they were destained with xylene and the antibodies detached with 3 M ammonium thiocyanate; then the specimens were treated for the demonstration of the other antigen. The procedure could be repeated and thus as many as four antigens could be demonstrated without damaging the tissues. Antigens participating in the immunohistochemical staining were well-preserved after destaining and detaching the antibodies as demonstrated by their ability to react again in a second staining.

ISSN:1052-0295    

DOI:10.3109/10520299109109979

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

Sieve cells and sieve tube members can be macerated from the phloem of various organs of woody and herbaceous species by au-toclaving the tissue in a mild macerating medium. This treatment does not digest the primary walls or the callose deposits on the sieve areas and sieve plates of the sieve elements. These cells can then be recognized by the fluorescence of their callose after staining with aniline blue. Sometimes adjacent sieve elements fail to separate and one can observe details of their junctures.

ISSN:1052-0295    

DOI:10.3109/10520299109109980

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

Human, rat and mouse pituitary tissues have been examined electron microscopically in transmission (TEM), scanning-transmission (STEM) and scanning (SEM) modes for the surface appearance of the secretory granules in tissue sections. Cryofixed and cryosectioned tissue showed only slightly protruding granule profiles which had a smooth surface. Cryofixed, freeze-dried and Epon embedded pituitaries, on the other hand, demonstrated swollen and furrowed surfaces over the granules after contact with water. This topography could also be seen after glutaraldehyde fixation but less after post-fixation in OsO4. The surface alterations in the sections of pituitary secretory granules are thought to be due to differences in the homogeneity of the resin infiltration, leaving resin-free openings where water can enter. It also seems probable that the Epon resin is more influenced by water than has been previously assumed, based on the findings of efficient elimination of osmium from the granules after incubation of tissue sections in water for only 10 min.

ISSN:1052-0295    

DOI:10.3109/10520299109109981

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

Gas bubbles frequently are formed during freeze substitution, especially when tissues are warmed to room temperature. The problem arises largely from the extreme solubility of CO2in the freeze substitution solvent. Gas bubbles may be minimized by briefly transferring the tissue to freshly chilled solvent before warming to room temperature.

ISSN:1052-0295    

DOI:10.3109/10520299109109982

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

C.I. basic blue 75 in an aqueous alkaline solution stains the nuclei of mature anc immature erythroblasts bright red. Simultane ously, the stain colors the cytoplasm of erythro blasts blue in immature cells and purple in ma ture cells. Colors of the type described were noi found in other normal and abnormal hemato poietic cells.

ISSN:1052-0295    

DOI:10.3109/10520299109109983

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor

摘要:

Ethidium bromide, (0.1% solution in ethanol-water, 1:3, v/v) was used to stain nuclei in mycelia and spores of different fungi. Nuclei looked bright brick red under green excitation. This method is very efficient, specific, reproducible and cost-effective.

ISSN:1052-0295    

DOI:10.3109/10520299109109984

出版商:Taylor&Francis    

年代:1991

数据来源: Taylor