ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018216

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

摘要:

SUMMARYOne-year-old dried cultures of 4 microsclerotial isolates, 1 white mycelial variant (no microsclerotia), and 1 isolate of the DM form (no microsclerotia) ofVerticillium albo-atrumwere examined to determine the origin of new growth from shavings transferred to sterile distilled water or to water agar. The microsclerotial isolates produced 3 structures that gave rise to new growth after the dormant period. These were clusters of hyaline microsclerotial cells, hyaline chains of chlamydospore-like cells, and hyaline hyphae intertwined in microsclerotial masses. In no instance were the melanized, thick-walled microsclerotial cells observed to germinate. The white-mycelial variant produced hyaline chlamydospore-like cells, either occurring in chains or singly, and hyaline hyphae which give rise to new growth. New growth in the DM form of the fungus occurred only from hyaline hyphae. New growth in dormant cultures could not be attributed to germination of conidia. Conidia from microsclerotial forms completely lost viability when stored 19 days in water or when dried for 20 minutes on glass slides at 22 C. Attempts with tetrazolium salts and indigo carmine to determine viability of melanized, thick-walled microsclerotial cells were unsuccessful, but microscopic examination of old and young microsclerotial cells suggested that a large number of the old dark cells were not living. These observations suggest that the principal means of survival of the fungusV. albo-atrumunder adverse conditions is in the various hyaline structures produced by the fungus rather than the melanized, thick-walled microsclerotial cells.

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018217

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

摘要:

SUMMARYThe chytrid,Dictyomorpha dioica, was found capable of infecting 8 identified species ofAchlya, 2 unidentified isolates ofAchlya, andThraustotheca clavata.All isolates tested ofSaprolegnia, Isoachlya, Dictyuchus, andCalyptralegniawere resistant to the parasite. The absence of a uniform pattern of infection among isolates of the same host species suggests the existence of biological races of the parasite or of its host. The optimal temperature for infection and sporulation ofD. dioicainAchlya flagellatawas 25 C. The age of the host culture at which maximal infection occurred was 24 hr.

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018218

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

摘要:

SUMMARYThe pure culture of plasmodia ofPhysarum flavicomumin semidefined media is reported. The decline of growth of the plasmodia on the semidefined medium and the apparently inhibitory effect of certain vitamins is discussed in relation to the stimulation of growth by high concentrations of quinic acid. High concentrations of quinic and gallic acids also stimulate sporulation in pure culture. The sporangia so induced contain viable spores which readily germinate on appropriate media. The amoebae have not been induced to multiply and grow independently, but will give rise to plasmodia in the absence of multiplication. These plasmodia may be subsequently cultured to complete the life cycle in pure culture.

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018219

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

摘要:

SUMMARYThe conidial stage ofConiochaeta ligniariais described. In morphology, it resembles the imperfect stages of several otherConiochaeta species.A typical mitotic cycle was found to be operative in the hyphae. The chromosome number, based on mitotic prophase (prometaphase) and metaphase counts, tentatively is 6. Anaphase irregularities, apparently frequent, are described.

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018220

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018221

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018222

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018223

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

摘要:

SUMMARYThree new species ofPythiumare described. The proposed names reflect some of their conspicuous peculiarities:P. coloratum—the yellow oospore and its very thick bluish-lilac wall;P. dissimile—small, usually toruloid compound sporangia and small plerotic oogonia without antheridia;P. pyrilobum—large oogonia and large pyriform sporangial elements combined to hyphal elements without septa.P. pyrilobumis also conspicuous because of its numerous antheridia and entanglements of their stalks, and its distinctly yellow oospores. Each of the latter two species possesses variously shaped sporangia, thus supporting arguments against division of the genus on the basis of sporangial shape.

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018224

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

摘要:

SUMMARYAerosol and subcutaneous vaccination with killed arthrospores and subcutaneous vaccination using a boivin-type fraction were compared for their efficacy in protecting rhesus monkeys against lethal aerosol challenge withC. immitis.Complete protection against lethal challenge was produced in the 6 monkeys subcutaneously vaccinated with killed arthrospores. Four of the 6 monkeys in each of the other immunized groups also survived. Only 2 of the 6 challenged controls survived. All animals were infected as a result of challenge. No dissemination was observed in animals immunized with the boivin-type fraction. The skin test reaction may be useful in detecting a response to immunization, but thus far the agar gel precipitin and CF reactions have not proved useful in detecting resistance to infection.

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018225

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor