摘要:

SUMMARYA single auxin was present in the sporangiophores and mycelium ofPhycomyces blakesleeanus, and was secreted into the synthetic culture medium containing asparagine as sole nitrogen source. It was found in the acid fraction, and was identified as indoleacetic acid by bioassay and chromatography. The amounts produced were at least 0.03μg/g fresh weight of sporangiophores, and 0.001-0.002μg/ml of culture medium. The Ehrlich and Salkowski reagents gave positive reactions for IAA only on some of the chromatograms although enough auxin was present to give clearly detectable spots. This erratic behavior, especially towards the Salkowski reagent, was probably due to interference by contaminating reducing compounds, which were indeed detected in the extracts. Preliminary evidence suggests the presence of indole-carboxylic acid in the sporangiophores. Unidentified Salkowski- and Ehrlich-positive substances were always found at high Rf in the acid and neutral fractions from the sporangiophores and medium, but were absent from the acid fraction of the mycelium.

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018256

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018257

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018258

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018259

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

摘要:

SUMMARYTwoArchangiumisolates,A. primigeniumandA. gephyra, were secured from soil and elm tree bark. Pure cultures were established and maintained on a variety of media. Agar plates containing rabbit dung and rabbit dung decoction agar proved most useful. The cultures were incubated at 22–26 C.Vegetative rods ofA. gephyraaveraged 10.5×0.6μ, whileA. primigeniumaveraged 7×1.2μ. Cells were needle-like, tapering slightly to blunt, rounded ends. Characteristic gliding and oscillating movements were observed for both species.Colonial morphology was similar for both species. A flat, thin sheet of cells with a microscopically irregular periphery was typical. The texture of the colonial mass varied in consistency.Fructification was observed in liquid and on solid media. In a liquid medium, atypical, star-like clusters culminated in a solid mass of shortened rods. On solid media, circular and wave-like masses of cells joined to form a mound of cells which matured into the typical, tubular, fruiting body mass.Fruiting bodies from soil or bark averaged 1 mm2or smaller in size. When grown in pure culture, fruiting bodies coalesced to cover areas of over 20 mm2. Fruiting bodies were usually enveloped in a slime layer. Encysted rods ofA. primigeniumaveraged 4.5×0.9μin size andA. gephyra2.5×1.4μ.Germination of fruiting bodies followed either of two patterns both resulting in normal, migrating colonies. Activated rods either pulsed forth from a germinating fruiting body or projected perpendicularly from the surface of the entire fruiting body as quills on a porcupine.Carotene as a component ofArchangiumpigmentation was ascertained by using conc. H2SO4. Loss of pigmentation by a fruiting body indicated loss of viability. Standard nuclear stains produced dense areas within vegetative cells and granulations. Granulations also occurred when5%methylene blue was employed. All rods were gram-negative and non-acid fast.

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018260

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018261

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018262

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018263

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

摘要:

SUMMARYBacteria are frequently associated with freshly ejected ascospores ofHypoxylon pruinatum, and are capable of reducing or preventing ascospore germination. In Minnesota 72% of the cankers had perithecia contaminated with bacteria and bacteria increased when stromata were kept moist continuously. When bacteria were suppressed spore germination was increased as much as 94%. Bacteria isolated from healthy bark also inhibited ascospore germination and may prevent establishment ofHypoxylon pruinatumin aspen.

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018264

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1965.12018265

出版商:Taylor&Francis    

年代:1965

数据来源: Taylor