ISSN:0027-5514    

DOI:10.1080/00275514.1966.12018294

出版商:Taylor&Francis    

年代:1966

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1966.12018295

出版商:Taylor&Francis    

年代:1966

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1966.12018296

出版商:Taylor&Francis    

年代:1966

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1966.12018297

出版商:Taylor&Francis    

年代:1966

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1966.12018298

出版商:Taylor&Francis    

年代:1966

数据来源: Taylor

摘要:

SUMMARYA large fraction of the total uptake of tracer cerium by washed resting yeast cells occurs quite rapidly (less than 15 min time). Optimum uptakes occur in acidic environments (pH 2.5-4.0). The cells show a relatively constant cerium binding capacity (about 3×10-4mg/mg dry wt), measured by radioactive tracer techniques after 2 hr contact time. The uptake is independent of temperature over the range from 4 to 38 C, is as high under anaerobic as under aerobic conditions, and is not affected by the common inhibitors of respiratory and glycolytic pathways. The cerium of labeled cells is rapidly removed by the hydrogen ion, complexing agents, and an excess of cerium cations. The data presented suggest cerium uptake by yeast involves primarily an adsorption of the element by some solid phase in or on the cell rather than an active transport process in which metabolic energy is required.

ISSN:0027-5514    

DOI:10.1080/00275514.1966.12018299

出版商:Taylor&Francis    

年代:1966

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1966.12018300

出版商:Taylor&Francis    

年代:1966

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1966.12018301

出版商:Taylor&Francis    

年代:1966

数据来源: Taylor

ISSN:0027-5514    

DOI:10.1080/00275514.1966.12018302

出版商:Taylor&Francis    

年代:1966

数据来源: Taylor

摘要:

SUMMARYCunninghamella echinulatahas the ability to utilize each even-chain fatty acid from acetate to stearate, and each odd-chain acid tested, as a sole source of carbon. Intermediates of theβ-oxidation pathway have been isolated from cultures of this organism grown on the various fatty acids. Acetic, butyric, caproic, caprylic, capric, lauric, and myristic acids have been identified from extracts of cultures grown on palmitate. Extracts of laurate-grown cultures were found to contain acetic, butyric, caproic, and capric acids. Butyric and acetic acids were detected in relatively large amounts in caproate-grown cultures; in addition, 2-hexenoic and,β-hydroxybutyric acids were also identified. Propionic and valeric acids were detected in extracts of heptanoate-grown cultures. The finding of all the saturated even-chain acids expected from the degradation of palmitate, laurate and caproate, and of both the saturated odd-chain acids expected from heptanoate, clearly supports the successive loss of 2-carbon fragments. Furthermore, the occurrence ofα-β-unsaturated and,β-hydroxy acids in the caproate cultures, combined with the other data, provides convincing evidence for the,β-oxidation of fatty acids by this fungus.

ISSN:0027-5514    

DOI:10.1080/00275514.1966.12018303

出版商:Taylor&Francis    

年代:1966

数据来源: Taylor