ISSN:0960-8931    

出版商:OVID    

年代:1995

数据来源: OVID

ISSN:0960-8931    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

Metastasis by thin melanomas is uncommon and unpredictable. In order to assess the prognostic value of p53 expression, p53 immunohistochemical staining was evaluated in 20 thin melanomas with documented metastasis and in 20 control tumours which failed to metastasize. Tumours selected were less than 1 mm thick and were individually matched for tumour thickness, date of excision and patient age and sex. The relative risk of metastasis given p53 overexpression was 1.5 (95% confidence interval 0.4–5.3; p = 0.53). Further quantitative analysis for p53 between the two groups did not demonstrate a significant difference (p-0.08). These results are consistent with there being no association between p53 overexpression in thin melanomas and risk of metastasis, however, the sample size was small, and the existence of such an association cannot be ruled out with confidence. For the 20 thin melanomas which metastasized, there was no association between the proportion of cells positive for p53 and length of the relapse-free period (correlation coefficient = 0.02, p = 0.94).

ISSN:0960-8931    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

The effect of hyperthermia, 500C applied for 5 min, on the development of lesions in hamster Greene melanomas was investigated. Hyperthermia was induced by a laser that produced radiation at 780–880 nm. Hamster melanomas were also examined after arrest of the blood circulation to differentiate between heat-induced lesions and those caused by ischaemia due to vascular occlusion. Tumours were removed 5 and 30 min and 1, 3, 6 and 24 h after thermotherapy. The cytotoxic effects of heat and ischaemia were examined by light and electron microscopy. Hyperchromatic nuclear staining, the first hyper-thermia-induced lesion, was detected in the superficial layers of the tumour 5 min after heat treatment. The lesions had progressed to severe pyknosis and extended into deeper layers of the tumour 1 h after hyperthermia. At 24 h necrosis was observed at a depth of 6 mm. The hyperthermia-induced lesions differed markedly from the ischaemia-induced lesions, in that the latter showed early mitochondrial damage but nuclear pyknosis did not become manifest until 3 h after arrest of the blood circulation.

ISSN:0960-8931    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

A panel of 13 murine monoclonal antibodies (mAbs) recognizing antigens on human melanoma cells but not on melanocytes was generated. Two mAbs (LHM3 and LHM5) stained sections of melanoma but not normal tissues. ′Abs LHM2 and LHM8 stained only a minority of normal′ tissues. The mAbs differed further in their staining patterns on melanoma cell lines HMB2, DX3 and SK23 in, FACS. The mAbs recognize antigens of 34, 38, 57, 94,190−, 200 and > 200 kD. One mAb each bound to each of the antigens HLA OR (LHM4) and high molecular weight proteoglycan (LHM2). The high molecular weight proteogly- ' can-specific mAb was used to construct a single-chain Fv i (scFv) antibody fragment and an antibody fusion phage in] Escherichia coli. Both the scFv and the fusion phage were shown to bind specifically to melanoma cells. A method for the selection of melanoma cell-binding phages from ' phage libraries is described.

ISSN:0960-8931    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

Family history is an important risk factor for cutaneous malignant melanoma. We evaluated the clinical characteristics of patients with cutaneous familial melanoma. A chart review was conducted, including all patients who presented to Massachusetts General Hospital Pigmented Lesion Clinic over an 8-year period. A total of 102 patients from 49 families were confirmed with shaving cutaneous melanoma. Eighty-two per cent had a personal and/or family history of dysplastic naevi. Within families, subsequent affected patients had thinner primary lesions with substantially lower median thickness than those diagnosed first. Seventeen per cent had multiple primary melanomas, with the median thickness of subsequent primaries being much lower than that of the first primary lesions. The mean thickness of primary lesions in patients with one primary lesion was marginally significantly greater than that in patients with multiple primaries. Verification of family history is essential given the implications of a positive family history. Screening family members of all patients with cutaneous melanoma and surveillance examinations for all patients with cutaneous melanoma is recommended.

ISSN:0960-8931    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

Fotemustine and dacarbazine constitute the most active single chemotherapeutic agents in the treatment of melanoma. In this phase II study we evaluated the activity and toxicity of a combination of fotemustine, dacarbazine and vindesine as a means of increasing response rate and survival time. Between September 1989 and November 1993, 43 patients with advanced melanoma were treated with a combination of 100 mg/m2fotemustine on days 1 and 8, 250 mg/m2dacarbazine on days 15 and 16 and 2 mg/m2vindesine on days 15 and 16 as induction treatment. After a 5-week rest period, the patients exhibiting a response or stable disease received the same drugs administered once every 28 days as maintenance therapy until either progression or toxicity was observed. Among 41 evaluable patients, there were six complete responses and eight partial responses. The overall response rate was 32% (95% confidence interval: 18–46%), with 8 months median duration of response. Median survival time was 10 months. This regimen was well tolerated. From this large phase II study, we conclude that such a combination is active against advanced malignant melanoma and seems to be more effective than fotemustine or dacarbazine used alone, especially on visceral metastatic sites.

ISSN:0960-8931    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

Incidence, nature and cause of severe acute regional toxicity were studied in 181 patients who underwent normothermic (37–38oC) or 'mild' hyperthermic (38–40oC) isolated limb perfusion (ILP) with melphalan. The known risk factors for toxicity (sex, tissue temperature, blood gas values, isolation level and melphalan peak concentration) were analysed. Severe acute regional toxicity occurred in 30 patients (16%). The limb was painful, swollen, red and warm in 19, often with a smooth and glistening aspect. Blistering scattered over the extremity was seen in 11 cases. In another 11 patients, late blistering limited to the footsole or handpalm developed. Twenty-six patients with severe toxicity had undergone ILP at the iliac isolation level (p < 0.05). Sex and tissue temperature did not predict toxicity. Venous perfusate blood gas values were severely deteriorated in four patients; high calculated melphalan peak concentrations occurred in nine patients. Irreversible long-term morbidity as a sequence of severe toxicity occurred in 10 of the 30 patients. Only one of the 11 patients with late blisters limited to sole or palm developed long-term morbidity (p<0.05). Thus, the only risk factor for severe acute regional toxicity that could be identified was iliac isolation level. However, in 27 of the 30 patients two or more risk factors were found.

ISSN:0960-8931    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

All primary invasive cutaneous malignant melanomas (CMM) diagnosed in Victoria and New South Wales from 1985 to 1989 were obtained from the population-based cancer registries. Altogether 14,590 people with first CMMs were followed for at least 2 years, during which time 496 multiple primary CMMs were identified. Of the study population, 3.4% developed a second primary CMM and 0.3% developed three or more. It was estimated that 4.5% of people would develop a second CMM within 5 years of the first and that the risk was higher in males, particularly in men aged over 70 years. With regard to metachronous primaries, only age and thickness of the first primary were significant predictors of the thickness of the second: older people tended to have thicker CMMs and second CMMs were generally thinner than the first. Body site concordance was higher than expected by chance, particularly for synchronous diagnoses. The high degree of site concordance of metachronous primaries lent support to the hypothesis that skin adjacent to the first CMM might have undergone a 'field effect', rendering it at increased susceptibility to malignancy.

ISSN:0960-8931    

出版商:OVID    

年代:1995

数据来源: OVID

ISSN:0960-8931    

出版商:OVID    

年代:1995

数据来源: OVID