摘要:

TheN‐terminal peptide of salmon proopiocortin has been isolated and the primary structure including two disulfide bonds elucidated. The peptide consisted of 76 amino acid residues, which is 27 residues shorter than the bovine and human peptides. TheN‐terminal 44 residues of the teleost peptide exhibited significant sequence homology to those of the mammalian peptides. The salmon peptide, however, is lacking in the counterpart of gamma‐MSH which is located between residues 51 and 64 in the mammalian pep

ISSN:0367-8377    

DOI:10.1111/j.1399-3011.1981.tb02975.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Alpha amylase (Sigma type IIA) was cleaved by cyanogen bromide. The sequences of two fragments, D which contains 88 amino acids and E which contains 56, are presented. By applying the Chou‐Fasman rules, D is predicted to have 32% beta sheet and 37% alpha helix, and E is predicted to exhibit 11% beta sheet and 71% alpha heli

ISSN:0367-8377    

DOI:10.1111/j.1399-3011.1981.tb02976.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

The synthesis and isolation of symmetrical anhydrides ofNα‐9‐fluorenylmethyloxycarbonyl (Fmoc) amino acids using water soluble carbodiimide is described. These compounds were used in a solid phase peptide synthesis of methionine‐enkephalin on a p‐benzyloxybenzyl ester polystyrene 1% divinylbenzene resin support. Homogeneous free pentapeptide was obtained in 42% overall yield. The Fmoc amino acid symmetrical anhydrides were stable during prolonged storage (2 years at 0°) and offer advantages over present “Fmoc solid phase” methods which use anhydrides f

ISSN:0367-8377    

DOI:10.1111/j.1399-3011.1981.tb02977.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Beta‐endorphin has been isolated from equine pituitaries. Its amino acid sequence is identical to that of ovine, bovine and camel β‐endorphins except for substitution of the threonine residue at position 6 by serine. The equine β‐endorphin has also been synthesized by the solid‐phase method. In comparison with the human hormone, equine β‐endorphin was shown to possess 3 times the receptor‐binding activity in rat membrane preparations and 1.6 times the analgesic potency in the mouse ta

ISSN:0367-8377    

DOI:10.1111/j.1399-3011.1981.tb02978.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

The modified Strecker synthesis of Patel&Worsley was combined with an enzymatic deamidation in the final step, to produce the title compound. The amino acid is a member of the family of the “fat” amino‐acids with high lipophilicity and space‐filling properties, as estimated by its Hansch hydrophobicity constant and its Charton steric parameter v. It can be used for qualitative and quantitative studies of structure‐activity relationships in biologically active peptides. Three of its derivatives suitable for peptide synthesis are also

ISSN:0367-8377    

DOI:10.1111/j.1399-3011.1981.tb02979.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Conformational energy calculations were carried out on the peptide enkephalins (ENK) and selected analogs to find those conformers of low energy. The analogs studied include [D‐Ala2]Enk‐NH2, [D‐Ala2]Enk, [D‐Met2, Pro5]Enk‐NH2, [D‐Ala2, D‐Phe5]Enk, [D‐Ala2, D‐Leu5]Enk, [D‐Ala2, (N‐Me)Phe4, Met5] Enk‐NH2and [D‐Ala2, (N‐Me)Met5]Enk‐NH2. When the low‐energy conformers for all the analogs are compared, different allowed backbone conformations are found which orient the functional side‐chains such that three classifications of structures appear. Each classification shows a unique configuration of side‐ chain positions in space even though different backbone conformations a

ISSN:0367-8377    

DOI:10.1111/j.1399-3011.1981.tb02980.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Protected secretin, a 27‐peptide amide, was synthesized by the all‐repetitive excess mixed anhydride (REMA) method and purified by preparative reverse‐phase high‐performance liquid chromatography. Highly potent secretin was obtained after deprotection with the aid of HF/anisole and purification by ion‐exchange chromatography. The scope of the REMA‐strategy is discussed in comparison with other

ISSN:0367-8377    

DOI:10.1111/j.1399-3011.1981.tb02981.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

[Aspartoyl3]‐, [β‐Asp3]‐secretin and secretin (4–27) were prepared. These peptides, products of intramolecular reactions in secretin, proved to be of low biological potency. The compounds could be separated by reverse‐phase high‐ performance liquid ch

ISSN:0367-8377    

DOI:10.1111/j.1399-3011.1981.tb02982.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

The solid phase synthesis of fragments 75–120‐NH2and 73–128‐GlyNH2of the human growth hormone sequence is described. Purification was by solvent extraction, gel filtration and partition chromatography. No growth‐promoting activity was detected in any of the peptides by the rat body‐growth assay. An immunochemically reactive area was located in the sequence 73–128 when tested by complement fi

ISSN:0367-8377    

DOI:10.1111/j.1399-3011.1981.tb02983.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

The a‐helix content of human β‐endorphin (βh‐EP) has been determined by circular dichroism (CD) in solutions ranging from 0 to 95% methanol in water. In addition, the CD spectra of βh‐EP‐(1–30),‐(1–29),‐(1–28),‐(1–27),‐(1–26), ‐(1–21) and ‐(1–15) have been examined in 90% methanol, and their a‐helix contents estimated using parameters determined for this solvent. Addition of methanol to βh‐EP solutions brings about a noncooperative formation of a‐helix. An attempt to correlate secondary structure in methanol with biological and immunological activities showedlimited direct correspondence, but may indi

ISSN:0367-8377    

DOI:10.1111/j.1399-3011.1981.tb02984.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY