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1. |
AMINO ACID COMPOSITION AND SEQUENCE ANALYSIS OF SAUVAGINE, A NEW ACTIVE PEPTIDE FROM THE SKIN OFPHYLLOMEDUSA SAWAGEI |
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International Journal of Peptide and Protein Research,
Volume 18,
Issue 2,
1981,
Page 113-120
PIER CARLO MONTECUCCHI,
AGNES HENSCHEN,
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摘要:
The complete amino acid sequence of sauvagine, a new active polypeptide from the skin ofPhyllomedusa sauvagei, a frog of Central and South America, has been determined by automated liquid‐phase procedure after specific removal of theN‐terminal pyrrolidonecarboxylic acid, and specific cleavages at the single methionine and at the two arginine residues. The proposed sequence is: Pyr‐Gly‐Pro‐Pro‐Ile‐Ser‐Ile‐Asp‐Leu‐Ser‐Leu‐Glu‐Leu‐Leu‐Arg‐Lys‐Met‐Ile‐GIu‐Ile‐Glu‐Lys‐Gln‐Glu‐Lys‐Glu‐Lys‐Gln‐Gln‐Ala‐Ala‐Asn‐Asn‐Arg‐Leu‐Leu‐Leu‐Asp‐Thr‐Ile‐NH2.Sauvagine possesses a number of pharmacological actions on diuresis, the cardiovascular system and endocrine glands; it can be considered the prototype of a new family of amphibian peptides, in addition to t
ISSN:0367-8377
DOI:10.1111/j.1399-3011.1981.tb02047.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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2. |
OCCURRENCE OF ASPARAGINE IN “DISALLOWED” CONFORMATIONS IN PROTEINS |
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International Journal of Peptide and Protein Research,
Volume 18,
Issue 2,
1981,
Page 121-126
V. RAVICHANDRAN,
E. SUBRAMANIAN,
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摘要:
A survey of 37 proteins has confirmed the frequent occurrence of asparagine in conformations having φ<180°, a region of the Ramachandran plot generally disallowed for amino acids with side chains possessing Cβatom and beyond. Model building studies suggest the possibility of an intramolecular hydrogen bond between the amino group of the asparagine side chain and the (backbone) carbonyl oxygen atom of the same resid
ISSN:0367-8377
DOI:10.1111/j.1399-3011.1981.tb02048.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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3. |
STUDIES OF PEPTIDE ANTIBIOTICS: |
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International Journal of Peptide and Protein Research,
Volume 18,
Issue 2,
1981,
Page 127-134
NOBUO KOSUI,
TETSUO KATO,
NOBUO IZUMIYA,
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摘要:
Tyrocidine A (TA) is an antibiotic cyclic decapeptide with the sequence of cyclo (‐L‐Val1‐L‐Orn2‐L‐Leu3‐D‐Phe4‐L‐Pro5‐L‐Phe6‐D‐Phe7‐L‐Asn8‐L‐Gln9‐L‐Tyr10‐). Gramicidin S (GS) regarded as a homolog of TA is also a cyclic decapeptide with the sequence of cyclo (‐L‐Val1‐L‐Orn2‐L‐Leu3‐D‐Phe4‐L‐Pro5‐ L‐Val6‐L‐Orn7‐L‐Leu8‐D‐Phe9‐L‐Pro10‐). GS shows higher antibacterial activity, whereas TA exhibits inhibitory activity on the biosynthesis of RNA. Two analogs of TA, [L‐Val6]‐TA (12a) and [L‐Orn7]‐TA (12b), were synthesized by the conventional method in order to study the interrelationships between the two related antibiotics TA and GS. Antibacterial activities of 12a and TA are nearly the same, but the activity of 12b is significantly lower. The optical rotatory dispersion spectra of 12a, 12b, and TA showed a trough at 233 nm region; the troughs of 12a and TA are nearly the same in depth, but the trough of 12b is shallower. Relatio
ISSN:0367-8377
DOI:10.1111/j.1399-3011.1981.tb02049.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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4. |
ISOLATION AND CHARACTERIZATION OF β‐LIPOTROPIN FROM THE PITUITARY GLAND OF THE OSTRICH,STRUTHIO CAMELUS |
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International Journal of Peptide and Protein Research,
Volume 18,
Issue 2,
1981,
Page 135-137
RYNO J. NAUDÉ,
WILLEM OELOFSEN,
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摘要:
Avian β‐lipotropin (β‐LPH) was purified from adenohypophyseal glands of the ostrichStruthio camelusby a procedure involving acid/acetone extraction, NaCl fractionation, CM‐cellulose chromatography, Sephadex G‐75 chromatography and paper electrophoresis (pH 6.7). The 90‐amino acid peptide behaved as a single substance during polyacrylamide‐gel electrophoresis, isoelectric focusing (pI of 6.0) andN‐terminal analysis, theN‐terminal amino acid being alanine. Ostrich β‐LPH exhibited lipolytic activity corresponding to an average minimal effective dose of 0.088 μg in
ISSN:0367-8377
DOI:10.1111/j.1399-3011.1981.tb02050.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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5. |
β‐LIPOTROPIN: PRIMARY STRUCTURE OF THE HORMONE FROM THE OSTRICH PITUITARY GLAND |
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International Journal of Peptide and Protein Research,
Volume 18,
Issue 2,
1981,
Page 138-147
RYNO J. NAUDÉ,
DAVID CHUNG,
CHOH HAO LI,
WILLEM OELOFSEN,
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摘要:
The amino acid sequence of β‐lipotropin from the ostrich pituitary has been determined. It consists of 79 amino acids. The amino acid 10sequence has been determined as follows: H‐20Ala‐Leu‐Pro‐Pro‐Ala‐Ala‐Met‐Leu‐Pro‐Ala‐30Ala‐Ala‐Glu‐Glu‐Glu‐Glu‐Gly‐Glu‐Glu‐Glu‐40Glu‐Glu‐Gly‐Glu‐Ala‐Glu‐Lys‐Glu‐Asp‐Gly‐50Gly‐Ser‐Tyr‐Arg‐Met‐A rg‐His‐Phe‐Arg‐Trp‐Gln‐60Ala‐Pro‐Leu‐Lys‐Asp‐Lys‐Arg‐Tyr‐Gly‐Gly‐70Phe‐Met‐Ser‐Ser‐Glu‐Arg‐Gly‐Arg‐Ala‐79Pro‐Leu‐Val‐Thr‐Leu‐Phe‐Lys‐Asn‐Ala‐Ile‐Val‐Lys‐Ser‐Ala‐Tyr‐Lys‐Lys‐Gly‐Gln‐OH. When compared with the primary s
ISSN:0367-8377
DOI:10.1111/j.1399-3011.1981.tb02051.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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6. |
COMPARISON OF THE STRUCTURAL FEATURES OF CORTICOTROPIN REQUIRED FOR STIMULATION OF STEROIDOGENESIS AND cAMP PRODUCTION IN RAT AND RABBIT ADRENOCORTICAL CELLS |
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International Journal of Peptide and Protein Research,
Volume 18,
Issue 2,
1981,
Page 148-153
S.M. RYBAK,
J. RAMACHANDRAN,
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摘要:
The steroidogenic activities of ACTH, α‐MSH, β‐MSH as well as analogs of the hormones have been compared in rat and rabbit adrenocortical cells. ACTH is equally active in both species and the melanotropins have very low steroidogenic potency in either species. The steroidogenic potencies of the peptide analogs are strikingly similar in the two species, suggesting that the structural requirements for eliciting steroidogenesis are the same in rat and rabbit adrenocortical cells. The analog NPS‐ACTH has low, comparable steroidogenic activity in both species. NPS‐ACTH is a potent antagonist of ACTH‐induced cAMP production in rat adrenocortical cells but acts as a weak partial agonist in rabbit adrenocortical cells. These results suggest that steroidogenesis may be mediated by receptors different from those involved in the cAMP response observed at supraphysiological concentrati
ISSN:0367-8377
DOI:10.1111/j.1399-3011.1981.tb02052.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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7. |
COMPARATIVE STUDY OF THE HIGH MOLECULAR WEIGHT PROTEIN FRACTION OF MUSTARD (B. JUNCEA) AND RAPESEED (B. CAMPESTRIS) |
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International Journal of Peptide and Protein Research,
Volume 18,
Issue 2,
1981,
Page 154-161
A. GURURAJ RAO,
M.S. NARASINGA RAO,
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摘要:
A method is described for isolating the high mol. wt. protein fraction of mustard and rapeseed in a homogeneous form. The protein from the two species had nearly identical sedimentation coefficients, molecular weights, intrinsic viscosity and fluorescence emission spectrum. However, differences in the amino acid composition were observed. Data on circular dichroism and rate of hydrolysis by proteolytic enzymes suggested differences in the secondary structures and possibly amino acid sequence.
ISSN:0367-8377
DOI:10.1111/j.1399-3011.1981.tb02053.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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8. |
ANALOGS OF AMANIN |
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International Journal of Peptide and Protein Research,
Volume 18,
Issue 2,
1981,
Page 162-168
GIANCARLO ZANOTTI,
CHRISTIAN BIRR,
THEODOR WIELAND,
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摘要:
Iie3‐amaninamide (3‐R) and its diastereomeric sulfoxide (3‐S) are obtained by oxidation of the bicyclic thioether peptide 2 by hydrogen peroxide in acetic acid. 2 was prepared by an intramolecular Savige‐Fontana reaction of the linear octapeptide tert.‐butylester 4 whoseN‐terminal Boc‐Hpi residue on treatment with TFA loses the Boc group and reacts under thioether formation with the released cysteine‐SH. The concomitantly deprotected carboxyl terminus is coupled intramolecularly with the free amino group of the secocompound 5 using the MA or DCCI method, thus forming the homodetic peptide ring. Compounds 3‐R and 3‐S agree very well with analog samples in chiroptical behavior. Thioether 2 and sulfoxide 3‐R exert 50% inhibition of RNA polymerase II (or B) fromDrosophila melanogasterin 10–6M solution whereas Kiof 3‐S i
ISSN:0367-8377
DOI:10.1111/j.1399-3011.1981.tb02054.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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9. |
INHIBITION OF PORCINE ELASTASE II BY CHICKEN OVOINHIBITOR |
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International Journal of Peptide and Protein Research,
Volume 18,
Issue 2,
1981,
Page 169-179
M. VERED,
A. GERTLER,
Y. BURSTEIN,
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摘要:
Interaction of porcine elastase II with native and modified chicken egg‐white ovoinhibitor was studied by determining the residual activity of the partially inhibited enzyme and by direct measurement of the stoichiometry of interaction using affinity chromatography, electrophoresis and gel filtration. It was found that the chymotrypsin binding site that is not modified by mild oxidation withN‐chlorosuccinimide (Shechter et al., Biochemistry, (1977) 16, 992–997) is capable of binding elastase II as well. The binding of chymotrypsin and elastase II is mutually exclusive and the affinity for chymotrypsin is stronger. Binding of 2 mol trypsin as 1 mol elastase I by ovoinhibitor does not interfere with the binding of elastase II. There is also an indication that the second binding site for chymotrypsin is capable of forming a complex with an additional molecule of elastase II, but the binding is so weak that it could be detected only by electropho
ISSN:0367-8377
DOI:10.1111/j.1399-3011.1981.tb02055.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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10. |
STUDIES ON PEPTIDES |
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International Journal of Peptide and Protein Research,
Volume 18,
Issue 2,
1981,
Page 180-194
KENICHI AKAJI,
NOBUTAKA FUJII,
HARUAKI YAJIMA,
MOTOYUKI MORIGA,
MITSURU AONO,
ATSUSHI TAKAGI,
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摘要:
The heptacosapeptide amide corresponding to the entire amino acid sequence of gastrin‐releasing peptide (GRP) was synthesized by assembling seven peptide fragments followed by deprotection with a new reagent system, IM trifluoro‐methanesulfonic acid‐thioanisole in TFA. The deprotected peptide was purified by ion‐exchange chromatography on CM‐cellulose followed by partition chromatography on Sephadex G‐25. The latter was found to remove effectively the Met (O) derivative of GRP. The highly purified synthetic GRP was active as synthetic bombesin on the molar basis. A new carboxyl‐activating reagent, thiazoline‐2‐thione, was employed for preparation of the ne
ISSN:0367-8377
DOI:10.1111/j.1399-3011.1981.tb02056.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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