摘要:

Adult pig pancreatic endocrine cells were harvested by autodigestion without added enzymes. The isolated, crude cells were purified by mono-poly resolving medium (MPRM). The purity of the harvested cells was determined by dithizone staining and the number of pancreatic endocrine cells was counted. A large number of the cells were stained red with dithizone and showed a high viability and a good insulin secretory response to glucose stimulation. The average number of cells purified by MPRM was 3.40 ± 1.32 X 105cells/g pancreas and the number of dithizone-stained cells was 2.81 ± 1.09 X 105cells/g pancreas. The insulin secretion from the pancreatic endocrine cells was maintained throughout a 40-day observation period and high glucose stimulation induced an increase in insulin secretion from the cultured cells. In the cells purified by MPRM, light and electron microscopic studies showed the cells to be typical pancreatic endocrine cells. The present purification method using MPRM allowed us quickly to obtain a large amount of adult pig pancreatic endocrine cells from the unpurified preparations. It is thought to be useful for transplantation and biochemical or biological studies of adult pig pancreatic endocrine cells.

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID

摘要:

The junction between the main pancreatic duct and the accessory duct has been thought to be the site of fusion between the ducts of the ventral and the dorsal primordia of the pancreas. The aim of this study was to investigate the fusion point between the ventral and the dorsal pancreatic ducts and to determine whether there is any relationship between the configuration of the pancreatic ducts and the manner of embryological fusion. Pancrea-tography was performed at 22 consecutive autopsies. Immunohistochemical staining of pancreatic polypeptide (PP) was performed because PP cells were rich in the ventral pancreas but poor in the dorsal pancreas. We identified two types of fusion. In one type, the ventral and the dorsal pancreatic ducts fuse at their junction (one-point fusion). In the other type, the two ducts fuse not only at the proximal site but at a second, more distal site (two-point fusion). Analysis of the pancreatograms showed that the distance between the junction and the major papilla in two-point fusion is significantly shorter than in one-point fusion (p< 0.01). These results indicate a close correlation between the pattern seen on pancreatograms and the manner of embryological fusion.

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID