摘要:

An experiment was conducted to compare two methods, referred to as A and B, for measuring amylase, lipase, trypsin, and Chymotrypsin activities in samples of pancreatic juice and to investigate the effect of freezing and thawing on enzyme activities. Three growing pigs were used in this study. A catheter was surgically inserted into the pancreatic duct and a simple T-cannula was placed in the duodenum to allow the return of collected pancreatic juice. Pancreatic juice was collected for a total of 24 h and 27 pooled 8-h samples were used in the analyses. Following collection and pooling, the samples were frozen at −80°C, due to the large number of samples and analyses, and stored for 2 weeks. The samples were thawed and enzyme activities were determined with Method A. The samples were frozen again at −80°C, stored for 2 weeks, and rethawed and enzyme activities were determined with Methods A and B. There was a direct relationship between Method A and Method B for measuring amylase, trypsin, and chymotrypsin activity (R2= 0.95, 0.81, and 0.90, respectively). However, a poor relationship was observed between a titrimetric and a photometric method for determining lipase activity (R2= 0.42). A second freezing, storage, and thawing did not affect (p>0.3) the concentration of protein or amylase activity in pancreatic juice. However, the activities of Chymotrypsin, trypsin, and, especially, lipase were decreased by 29.0% (p<0.11), 40.4% (p<0.01), and 82.9% (p<0.001), respectively. In conclusion, either Method A or Method B can be used to measure amylase, trypsin, and Chymotrypsin activities in pancreatic juice but a titrimetric method should be used to determine lipase activity. Furthermore, if the activities of lipase, trypsin, and Chymotrypsin are to be determined, the sample should only be frozen and thawed once. If amylase activity will be determined, the sample can be repeatedly frozen and thawed.

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID

摘要:

Fecal elastase-1 is a candidate for a sensitive noninvasive test detecting chronic pancreatitis. This prospective study enrolled 10 healthy male controls and 23 patients referred for tube testing of pancreatic function. It was designed (a) to correlate duodenal outputs and fecal concentrations of elastase-1 with duodenal outputs of amylase, lipase, trypsin, and chymotrypsin in the fed state (duodenal perfusion of a mixed liquid meal at 2.5 kcal/min for 150 min), (b) to compare the diagnostic accuracy of fecal elastase-1 and fecal chyrnotrypsin, and (c) to characterize the cyclical pattern of postprandial pancreatic secretion in healthy subjects and patients with chronic pancreatitis. Based on their enzyme responses to duodenal meal perfusion and imaging procedures, 12 patients were classified as having normal pancreatic function and 11 patients as having chronic pancreatitis. Duodenal enzyme outputs of elastase-1 were markedly lowered in chronic pancreatitis (p<0.0001) and correlated well with the outputs of the other four enzymes (r>0.71,p<0.00001). Fecal concentrations of elastase-1 were also clearly reduced in chronic pancreatitis (p<0.0001). Fecal chyrnotrypsin was less strongly associated with duodenal enzyme outputs (r= 0.33 tor= 0.587), whereas fecal elastase-1 correlated more precisely with the duodenal outputs of all five enzymes (r= 0.637 tor= 0.830,p<0.00001). Sensitivity and specificity in the detection of chronic pancreatitis amounted to 0.64 and 0.95 for fecal elastase-1 and 0.27 and 0.95 for fecal chyrnotrypsin, respectively. In the postprandial state, peaks of enzyme secretion occurred at a frequency of about 1 peak/150 min. The amplitude but not the frequency of secretory peaks was markedly reduced in chronic pancreatitis (p<0.01). We conclude that fecal elastase-1 clearly exceeds the sensitivity of fecal chyrnotrypsin in the diagnosis of chronic pancreatitis but does not reliably detect all cases with mild to moderate disease. The pattern of postprandial pancreatic secretion is cyclical, even with minimal secretory outputs in chronic pancreatitis.

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID

摘要:

We described a rare form of giant cell tumor of the pancreas composed of mixed osteoclastic/pleomorphic-type giant cell tumor with ductal adenocarcinoma. Immunohistochemical study showed positive staining of cytokeratin and epithelial membrane antigen for pleomorphic-type giant cells and ductal adenocarcinoma but negative for osteoclastic-type giant cells. Vimentin stained positive in both types of giant cells but negative in adenocarcinoma. Osteoclastic-type giant cells were strongly positive for CD68 and tartarate-resistant acid phosphatase was present in these cells, suggesting the osteoclast-like character. CD68 was negative for both pleomorphic-type giant cells and ductal adenocarcinoma. From these findings, we consider that this tumor might be a carcinosarcoma-like neoplasm consisting of both an epithelial and a histiocytic-mesenchymal component.

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID

ISSN:0885-3177    

出版商:OVID    

年代:1997

数据来源: OVID