摘要:

ABSTRACTSporophytesof Ectocarpus siliculosus (Dillwyn) Lyngbye andE. fasciculatusHarvey were collected in the vicinity of Roscoff Brittany, France. Gametophytes derived from meiospores were used for intra‐ and interspecific crosses. Intraspecific gamete combinations gave viable zygotes, which developed into fertile sporophytes. Interspecific crosses were unsuccessful. Gamete fusions did not occur between female gametesof E. fasciculatusand male gametes of E. siliculosus.Hybrid zygotes were formed in the reciprocal combination but died soon after germination. We conclude that the two speciesof Ectocarpusat Roscoff represent distinct taxonomic entities, which are separated by pre‐ and postzygotic compatibility barriers. These biological findings are confirmed by the differential occurrence of the chemotaxonomic marker betaine‐lipid diacetylglycerylhydroxymethyltrimethyl‐β‐alanine, which is present in our cultures ofE. fasciculatusbut absent inE. s

ISSN:0022-3646    

DOI:10.1111/j.0022-3646.1995.00173.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

ABSTRACTA method for extracting DNA from laminarialean algae resulting in DNA of sufficient quantity and purity for DNA fingerprints is presented. The method both eliminates the use of liquid N2and delays the use of toxic chemicals in the initial extraction steps; thus, it is appropriate for use in remote field locations. The algal samples were ground in a solution of hexadecyltrimethylammonium bromide (CTAB) and polyvinylpyrrolidone (PVPP) at room temperature and then stored for 1 week in the CTAB‐PVPP solution at room temperature before extraction with chloroform‐isoamyl alcohol and purification by cesium chloride ultracentrifugation. No degradation of DNA was observed. Hybridization of RsaI‐digestedMacrocystis pyrifera (L.) C. Ag. DNA with theM13repeat probe yielded a DNA fingerprint with 12 discrete bands 4–19 kb in molecul

ISSN:0022-3646    

DOI:10.1111/j.0022-3646.1995.00177.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY