摘要:

Abstract.Lymphocytes were used as a cellular model for thein vitromeasurements of maximal ouabain binding sites in order to assess any changes in young men at increased risk of developing essential hypertension, and to analyse whether any such changes were associated to borderline hypertension and/or heredity.Four groups were evaluated; 28 normotensive (NTO) and 20 borderline hypertensive (BHO) offspring of hypertensives. Twelve borderline hypertensives (BH) and 28 normotensive subjects (NT) with normotensive parents.The number of ouabain binding sites were significantly increased in the borderline hypertensives irrespective of heredity.The borderline hypertensives were heavier than the normotensives. A stepwise multiple regression model was therefore used in order to control confoundings by body mass index (BMI) and other factors such as age, gamma glutamyl transferase, 24 h sodium excretion, serum triglyceride, and serum cholesterole, which may influence the number of ouabain binding sites. Only BMI entered the stepwise model.These results indicate the presence of an increased number of sodium‐potassium pumps in lymphocytes from borderline hypertensives. This difference may be attributed to the blood pressure disease or increased body mas

ISSN:0014-2972    

DOI:10.1111/j.1365-2362.1989.tb00309.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Abstract.The effect of an acute protein load (2 g kg‐1bodyweight [BW]) was studied in nine type 1 diabetic children. Patients were maintained on two different dietary regimens. In study one, patients were on a high protein diet providing from 2.7 to 1.8 g of protein/kg of BW per day. In study two, patients were reevaluated after three weeks of a diet providing from 1.0 to 1.2 g kg‐1of BW per day of protein. In study one (High Protein Diet), we failed to observe any rise in GFR and RPF following the protein meal (137 ± 21 basal vs. 110±14 and 472±93 basal vs. 494±93 ml/1.73 m2of SA min‐1at 60 min.This is in contrast with results from seven age matched controls consuming a free diet, which showed a significant rise in both GFR and RPF.In study two (low protein diet), basal GFR was significantly reduced. However after the protein load, both GFR (92±11 vs. 126±18 ml/1.73 m2of SA min‐1) and RPF (467±83 vs. 705±102 ml/1.73 m2min‐1) rose significantly (P<0.05 vs. basal). The data indicate that:1 short term protein restriction reduces significantly GFR in type 1 diabetic children;2 diabetic children maintained on an high protein intake show an altered haemodynamic response to protein ingestion;3 a normal response to protein ingestion can be restored by short term dietary pr

ISSN:0014-2972    

DOI:10.1111/j.1365-2362.1989.tb00310.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Abstract.Increasing evidence suggests that in experimental diabetes an impairment in Na+, K+–ATPase activity plays a central role in the pathophysiology of diabetic complications, while only a few data are available with regard to human subjects. We studied the erythrocyte membrane Na+, K+‐ATPase activity and membrane fluidity in insulin‐dependent and non‐insulin‐dependent diabetic subjects. A significant decrease in the enzyme activity and in fluorescence polarization values was found in both groups compared with normal subjects. Neither Na+, K+‐ATPase activity nor membrane fluidity was found to be related to metabolic control, assessed by means of fasting blood glucose levels and HbA1c. On the contrary, a significant correlation was observed between Na+, K+‐ATPase activity and membrane fluidity in both insulin‐dependent and non‐insulin‐dependent diabetic subjects. The present work provides evidence that a reduction in the Na+, K+‐ATPase activity is present in the plasma membranes of insulin‐dependent and non‐insulin‐dependent diabetics. Furthermore, it suggests that the change in enzyme activity might be related to modific

ISSN:0014-2972    

DOI:10.1111/j.1365-2362.1989.tb00311.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Abstract.In a prospective study with cross‐over design 20 patients with insulin‐dependent diabetes mellitus of more than 2 years duration were treated for 6 months with continuous subcutaneous insulin infusion (CSII) and multiple insulin injections (MII).Metabolic control, platelet aggregability, thromboxane B2levels in serum and plasma as well as antithrombin III (ATIII) activity and von Willebrand factor antigen were evaluated. A good metabolic control was obtained by both intensified regimens. No difference could be demonstrated between either platelet function tests or serum level of von Willebrand factor antigen during treatment with CSII and MII. However, the plasma level of ATIII activity was significantly higher (P<0.01) during MII treatment as compared to CSII treatment. There was no correlation between ATIII activity and daily insulin requirement or serum fructosamine.In conclusion, long‐term metabolic control with MII has a favourable effect on ATIII activity in plasma. This may be important for a delay in onset and progression of diabetic vascular complica

ISSN:0014-2972    

DOI:10.1111/j.1365-2362.1989.tb00312.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Abstract.Recent reports have described high levels of one or more substances which cross‐react with digoxin antibodies in the serum of women with pre‐eclampsia. We measured plasma ouabain‐like activity and intra‐erythrocyte sodium and potassium concentrations, in addition to performing routine hypertensive laboratory tests, in 13 normotensive non‐pregnant subjects, 15 normotensive pregnant women and 16 pre‐eclamptic women (gestational age:33–36 weeks). Plasma ouabain‐like activity, measured as plasma‐induced variations in ouabain binding to human erythrocytes, proved significantly higher in both groups of pregnant subjects as compared to normotensive non‐pregnant women, and a significant difference was also found between pre‐eclamptic and normotensive pregnant women, the former exhibiting higher plasma ouabain‐like activity. No differences in intracellúlar sodium and potassium levels were detected among the three groups studied. Though there is reason to believe that the high plasma levels found both in normal and hypertensive pregnancy may depend on placental production, we are not in a position to define with any degree of certainty what the mechanism or mechanisms are that regulate ouabai

ISSN:0014-2972    

DOI:10.1111/j.1365-2362.1989.tb00313.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Abstract.Sodium‐lithium countertransport (Na‐Li CT) has been reported to be increased in essential hypertension (EHT) but the nature and degree of distinction from normal controls is unclear. Of 44 unselected patients with EHT in the hospital hypertension clinic 36% had Na‐Li CT greater than the normal control range and 70% of these had a family history of hypertension. Almost all the patients with normal Na‐Li CT had no family history of hypertension. Analysis of variance showed that raised Na‐Li CT was related to both a family history of hypertension and a family history of a cardiovascular event. Of 23 patients with hypertension secondary to renal disease, 43% had Na‐Li CT greater than the normal control range and raised Na‐Li CT was related to both a family history of hypertension and a family history of cardiovascular event in the same way as EHT. Raised Na‐Li CT was not characteristic of EHT but identified a subgroup of patients with EHT and a family history of hypertension, some of whom also ha

ISSN:0014-2972    

DOI:10.1111/j.1365-2362.1989.tb00314.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY